Prt-4 and Prt-5: New constituents of a gene cluster on chromosome 7 coding for esterproteases in the submandibular gland of the house mouse (Mus musculus)

Abstract

The heredity and linkage of gene loci were established for two different enzymes with esterproteolytic activity from mouse submandibular gland: protease A and protease E. Based upon strain distribution and biochemical properties of the two esterproteases, the existence of two corresponding structural loci is proposed: Prt-4 (protease A) and Prt-5 (protease E). Prt-4 and Prt-5 proved to be different from Tam-1. From a four-point-cross, the gene order Gpi-1-(Tam-1, Prt-4, Prt-5)-c is suggested. Thus a gene cluster was shown to exist on chromosome 7 coding for esterproteases, all of which are controlled by testosterone.

Use of a cDNA recombinant for the γ-subunit of mouse nerve growth factor to localize members of this multigene family near the TAM-1 locus on chromosome 7

“…Our Southern analyses of mouse-hamster hybrid cell lines and recombinant inbred lines of mice, along with our message selection data, suggest that y-NGF and all serine proteases detectable with pSM676 are clustered at or near the Tam-l locus on mouse chromosome 7 as has previously been reported for other serine proteases (7,16). Our conclusion is further supported by the results of Mason et al (36) who isolated a genomic clone containing putative coding sequences for two kallikreins separated by less than 4,000 base pairs.…”

“…comm. ), proteases A & E (16), and TAM-1 (17), have previously been shown to map at or near the Tam-l locus on chromosome 7 in the mouse. We have chosen to study the genetic organization of the genes encoding these proteases with the aim of determining the size of this family and the extent to which the genes are clustered, and also with the goal of understanding how their coordinate and tissue specific expression is achieved.…”