Prunella vulgaris leaf extract assisted green synthesis of silver nanoparticles: Antimicrobial activity

Ganaie, Shakeel Ahmad; Zahoor, Irfana; Singh, Ruby

doi:10.1016/j.matpr.2022.09.520

Cited by 3 publications

(7 citation statements)

References 29 publications

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“…This result agrees well with the findings reported in other investigations [46][47][48][49][50]. Several researchers have attributed this color change characteristic of AgNP formation to the excitation of surface plasmon vibration in AgNPs [45,51,52]. On the basis of visual observations, the reaction mixture containing NPs synthesized with 1 mM AgNO 3 was very lightly colored (possibly due to a low concentration of NPs), whereas the reaction mixture containing NPs synthesized with 5 mM AgNO 3 was highly colored and showed deposits at the bottom of the tube, which indicated the presence of a high concentration of large particles [22].…”

Section: Characteristics Of Agnps Synthesizedsupporting

confidence: 93%

Synthesis of green-engineered silver nanoparticles using Cymbopogon citratus (lemongrass) and its antibacterial activity against clinical Pseudomonas aeruginosa

Arsene,

Davares,

Goriainov

et al. 2024

Int J One Health

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Background and Aim: The use of bioengineered nanocomposites as antimicrobials has increased in recent years, but very few investigations have been conducted to test their effectiveness against Pseudomonas aeruginosa, a pathogen presenting public health risks that can impact both humans and animals. The aim of this study was to assess the antimicrobial potential of phytofabricated silver nanoparticles synthesized using lemongrass extract against clinical isolates of P. aeruginosa. Materials and Methods: The extraction of active compounds from the leaves of Cymbopogon citratus was performed using ethanol (80%) as a solvent, high-performance liquid chromatography-mass spectrometry was used to analyze the chemical composition of the extract, the synthesis of silver nanoparticles (AgNPs) was done using silver nitrate (AgNO3) as a precursor, and the antimicrobial and antibiofilm activity of the extract and the AgNPs phytofabricated was assessed against 10 clinical strains of P. aeruginosa. Results: Lemongrass extract was found to consist of the following main compounds: Caffeic acid (445.21 ± 32.77 μg/g), p-coumaric acid (393.32 ± 39.56 μg/g), chlorogenic acid (377.65 ± 4.26 μg/g), quinic acid (161.52 ± 17.62 μg/g), and quercetin-3-glucoside (151.35 ± 11.34 μg/g). AgNPs were successfully phytofabricated using 2.5 mM AgNO3. The ultraviolet (UV)-visible absorption spectra of the AgNPs showed a localized surface plasmon resonance at 464 nm with an absorbance of 0.32 A. The 50x hydrodynamic diameter was 50.29 nm with a surface area value of 120.10 m2/ cm3, and the volume mean diameter and Sauter mean diameter were 50.63 nm and 49.96 nm, respectively. Despite the compound found in lemongrass extract, no antimicrobial activity was observed with the extract, while AgNPs exhibited noteworthy dose-dependent antimicrobial activity with inhibition diameters up to 24 mm and minimum inhibitory concentration (MIC) and minimum bactericidal concentration ranging from 2 to 16 and 4–64 µg/mL, respectively. AgNPs also demonstrated significant antibiofilm activity by inhibiting biofilms up to 99% between MIC/2 and 2MIC. Conclusion: The present study suggests that lemongrass is a good candidate for the synthesis of AgNPs with good physicochemical characteristics and having a strong anti-pseudomonas activity. Further research is needed to assess the stability and safety of these AgNPs. Keywords: antibiofilm capacity, antimicrobial activity, green synthesis, lemongrass, Pseudomonas aeruginosa, silver nanoparticles.

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Section: Characteristics Of Agnps Synthesizedsupporting

confidence: 93%

Synthesis of green-engineered silver nanoparticles using Cymbopogon citratus (lemongrass) and its antibacterial activity against clinical Pseudomonas aeruginosa

Arsene,

Davares,

Goriainov

et al. 2024

Int J One Health

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“…However, a serial dilution of BBE was reapplied to ensure the proper optimal extract concentration. Further, UV-Vis spectroscopy was applied in each step for the listed samples to explore the absorption spectra and characteristics of surface plasmon resonance (SPR) of the synthesized Ag NPs [ 57 , 58 ]. The screening and optimization process, depending on the initial screening data of the UV spectroscopy process, revealed the effectivity of the green synthesis of Ag NPs using PPE at 70 °C for an hour ( Figure 2 d), while the ideal selected volume ratio is 1:2 of extract:silver ions.…”

Section: Resultsmentioning

confidence: 99%

Comparative Study of Callistemon citrinus (Bottlebrush) and Punica granatum (Pomegranate) Extracts for Sustainable Synthesis of Silver Nanoparticles and Their Oral Antimicrobial Efficacy

Ismail,

Mohamed,

Elzwawy

et al. 2024

Nanomaterials

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A comparative study was applied to investigate the potential of Callistemon citrinus (bottlebrush) flower extract (BBE) and Punica granatum (pomegranate) peel extracts (PPE) for the sustainable synthesis of the silver nanoparticles, Ag-BBE and Ag-PPE, respectively. The synthesis process of Ag NPs using the selected extracts was applied under optimized conditions. Hence, the effect of the selected plant’s type on the different characteristics of the synthesized green Ag NPs was investigated. The UV-Vis spectroscopy revealed the presence of the characteristic silver peaks at 419 and 433 nm of the Ag-BBE and Ag-PPE, respectively. The XRD spectra reported the fcc phase formation of Ag NPs. The TEM results highlighted the morphological features of the synthesized Ag NPs. with a size range of 20–70 nm, and with 10–30 nm for Ag-BBE and Ag-PPE, correspondingly. The Raman spectra revealed characteristic silver bands in the Ag-PPE and reflected some bands related to the natural extract in the Ag-BBE sample. The antimicrobial activity and statistical analysis investigation were conducted against four selected oral pathogens (Staphylococcus aureus (SA), Candida albicans (CA), Staphylococcus epidermidis (S. epi), and Enterococcus faecalis (EF)). Both tested extracts, BBE, and PPE, revealed potential effectivity as reducing and capping agents for Ag NP green synthesis. However, the synthesized NPs demonstrated different features, depending on the used extract, reflecting the influence of the plant’s biomolecules on the nanoparticles’ properties.

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“…The reaction between the flower extracts of J. nudiflorum and AgNO 3 resulted in a visible color transition from yellowish to reddish-brown, indicating the successful green synthesis of AgNPs ( Figure 2 ). This color alteration was attributed to the surface plasmon resonance (SPR) of silver ions, which were reduced to stable AgNPs by active molecules in the extract [ 30 , 35 ]. At 1 h, the red-brown color was clearly observed in A1B1, A2B1, and A3B1, indicating the initiation of AgNP synthesis.…”

Section: Resultsmentioning

confidence: 99%

“…Moreover, peaks at 2930 cm −1 , 2923 cm −1 , 2922 cm −1 ,2921 cm −1 , 2900 cm −1 , and 2880 cm −1 are associated with the C-H stretching vibrations of alkanes [ 23 , 28 , 35 , 46 , 47 ]. The presence of aldehyde or ketone functional groups (C=O) from flavonoid and tannin derivatives can be inferred from the peaks at 1617 cm −1 ,1626 cm −1 , 1650 cm −1 , and 1653 cm −1 [ 44 , 47 , 49 ].…”

Section: Resultsmentioning

confidence: 99%

“…The presence of aldehyde or ketone functional groups (C=O) from flavonoid and tannin derivatives can be inferred from the peaks at 1617 cm −1 ,1626 cm −1 , 1650 cm −1 , and 1653 cm −1 [ 44 , 47 , 49 ]. While the peak at 1633 cm −1 and 1631 cm −1 signifies C=C stretching vibrations of the hydrocarbon portion of biological double-bond molecules, the band at 1052 cm −1 and 1031 cm −1 may be attributed to C-O stretching [ 35 , 46 , 50 ]. And the peaks at 1607 cm −1 and 1396 cm −1 correspond to the asymmetric stretching of NO 2 [ 50 ].…”

Section: Resultsmentioning

confidence: 99%

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Green Synthesis of Silver Nanoparticles Using Jasminum nudiflorum Flower Extract and Their Antifungal and Antioxidant Activity

Yang,

Guo,

Long

et al. 2023

Nanomaterials

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The synthesis of metal nanomaterials is a timely topic due to their widespread use in fields such as crop protection, the environment, medicine, and engineering. Green synthesis of nanoparticles, which uses plant extracts instead of industrial chemical agents to reduce metal ions, has been developed to decrease costs, reduce pollution, and improve environmental and human health safety. In this paper, silver nanoparticles (AgNPs) were synthesized from the flower extract of Jasminum nudiflorum. The green synthesized AgNPs were characterized by UV-Vis, FTIR, XRD, SEM, and other technologies. The antifungal activity of the prepared AgNPs against Alternaria longipes was tested using the plate method, the concentration dilution method, and other methods, and the antioxidant activity of the prepared AgNPs was evaluated by DPPH and hydroxyl free scavenging methods. The results showed that AgNPs synthesized from J. nudiflorum flower extract have a face-centered cubic structure (fcc), and the average grain size of the nanoparticles is 13 nm; they are also mainly spherical in shape. Additionally, the concentration of AgNPs (ranging from 16 to 128 μg/mL) significantly inhibited the mycelial growth of A. longipes in comparison to the control. The inhibitory rate gradually increased with increasing AgNP concentration, ranging from 70.64% to 79.60% at a concentration of 128 μg/mL. The minimum inhibitory concentration was observed at 32 μg/mL. AgNPs induced overaccumulation of MDA in A. longipes, resulting in cell membrane damage and nucleic acid leakage. Moreover, the AgNPs have significant antioxidant properties, which increase with increasing concentration. The clearance rate of DPPH was 25.46 ± 0.90% when the concentration of AgNPs was 8 μg/mL, and the clearance rate of the hydroxyl radical was 28.62 ± 0.59% when the concentration of AgNPs was 128 μg/mL. Thus, the flower extract from J. nudiflorum holds potential as an environmentally friendly and green alternative for the synthesis of AgNPs, which have antifungal and antioxidant potential.

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Prunella vulgaris leaf extract assisted green synthesis of silver nanoparticles: Antimicrobial activity

Cited by 3 publications

References 29 publications

Synthesis of green-engineered silver nanoparticles using Cymbopogon citratus (lemongrass) and its antibacterial activity against clinical Pseudomonas aeruginosa

Synthesis of green-engineered silver nanoparticles using Cymbopogon citratus (lemongrass) and its antibacterial activity against clinical Pseudomonas aeruginosa

Comparative Study of Callistemon citrinus (Bottlebrush) and Punica granatum (Pomegranate) Extracts for Sustainable Synthesis of Silver Nanoparticles and Their Oral Antimicrobial Efficacy

Green Synthesis of Silver Nanoparticles Using Jasminum nudiflorum Flower Extract and Their Antifungal and Antioxidant Activity

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