Prussian Blue/Calcium Peroxide Nanocomposites-Mediated Tumor Cell Iron Mineralization for Treatment of Experimental Lung Adenocarcinoma

Abstract

Current lung cancer diagnosis methods encounter delayed visual confirmation of tumor foci and low-resolution metrics in imaging findings, which delays the early treatment of tumors. Here, we developed a potent lung cancer imaging and treatment strategy centered around a nanotransformational concept of tumor iron mineralization in situ, which employs Prussian blue/calcium peroxide nanocomposites as a precursor. The resultant iron mineralization in tumor cells greatly facilitates the early and differential diagnosis of lung carcinoma from benign nodules via medical imaging, meanwhile introducing oxidative stress to activate the cellular apoptosis and ferroptosis pathways, resulting in inhibition of the malignant behavior of tumor cells. Tumor-microenvironment-triggered iron mineralization enables integration of the detection and prevention of tumor metastasis at its early stages with no assistance of toxic drugs, which offers a potential solution for the precise management of lung cancer with ideal outcomes.

Targeting ferroptosis as a vulnerability in pulmonary diseases

“…Moreover, the diagnosis and ferroptosis-targeted treatment of lung cancer via nano-particles have also made some progress. Prussian blue/calcium peroxide nanocomposites promote iron mineralization in lung cancers, which greatly facilitates early diagnosis of lung carcinoma and activates ferroptosis to inhibit tumor growth [ 117 ]. Most recently, a self-assembled pH-sensitive superparamagnetic iron oxide nanoclusters (SPIONCs) is reported to enhance in situ ferroptosis and apoptosis of lung tumors with radiotherapy and chemodynamic therapy via releasing iron in the tumor microenvironment (TME) [ 118 ].…”

PEGylated Prussian blue nanoparticles for modulating polyethyleneimine cytotoxicity and attenuating tumor hypoxia for dual-enhanced photodynamic therapy

“…Prussian blue (PB) was synthesized by adapting previous studies. [43][44][45] Typically, 0.367 g K 3 [Fe(CN) 6 ], 8 g PVP, and 90 mL HCl were mixed in 100 mL deionized water by stirring to obtain a clear solution at room temperature for 30 min. Next, the reaction proceeded at 80 1C for 20 h. The PB was collected after centrifugation (11 000 rpm, 15 min), followed by washing in deionized water.…”

A dual gate-controlled intelligent nanoreactor enables collaborative precise treatment for cancer nanotherapy

“…2b, a tetraline spectrum with a relative intensity of 1 : 2 : 2 : 1 was obtained for the mixture of HDHF and H 2 O 2 , which was attributed to the characteristic spectrum of BMPO/ • OH, validating the formation of • OH. 44 In addition, it has been proved that Pt-based nanomaterials could effectively catalyze H 2 O 2 into O 2 . 45 As illustrated in Fig.…”