PsbS‐dependent and ‐independent mechanisms regulate carotenoid‐chlorophyll energy coupling in grana thylakoids

Gacek, Daniel A.; Holleboom, Christoph-Peter; Tietz, Stefanie; Kirchhoff, Helmut; Walla, Peter Jomo

doi:10.1002/1873-3468.13586

Cited by 4 publications

(4 citation statements)

References 43 publications

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“…Despite the availability of a PsbS crystallographic structure (Fan et al, 2015), there is still a debate about its basic components. The presence of chlorophyll, xanthophyll (Correa-Galvis et al, 2016;Gachek et al, 2019) and its role as a luminal pH sensor (Bergantino et al, 2003;Li et al, 2004;Roach and Krieger-Liszkay, 2012;Liguori et al, 2019), are still debated. The primary role of PsbS is thought to be a protective one, as a key player in some aspects of non-photochemical quenching (NPQ) (Niyogi and Truong, 2013;Ruban et al, 2016;Bassi and Dall'Osto, 2021).…”

Section: Introductionmentioning

confidence: 99%

Bicarbonate Activation of Monomeric Photosystem II-PsbS/Psb27 Complex

Rutherford

Fantuzzi

Piano

et al. 2022

Preprint

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In thylakoid membranes, Photosystem II monomers from the stromal lamellae contain the subunits PsbS and Psb27 (PSIIm-S/27), while Photosystem II monomers from granal regions (PSIIm) lack these subunits. Here, we have isolated and characterised these two types of Photosystem II complexes. The PSIIm-S/27 showed enhanced fluorescence, the near-absence of oxygen evolution, as well as limited and slow electron transfer from QA to QB compared to the near-normal activities in the granal PSIIm. However, when bicarbonate was added to the PSIIm-S/27, water splitting and QA to QB electron transfer rates were comparable to those in granal PSIIm. The findings suggest that the binding of PsbS and/or Psb27 inhibits forward electron transfer and lowers the binding affinity for the bicarbonate. This can be rationalized in terms of the recently discovered photoprotection role played by bicarbonate binding via the redox tuning of the QA/QA?- couple, which controls the charge recombination route, and this limits chlorophyll triplet mediated 1O2 formation (Brinkert K et al. (2016) Proc Natl Acad Sci U S A. 113(43):12144-12149). These findings suggest that PSIIm-S/27 is an intermediate in the assembly of PSII in which PsbS and/or Psb27 restrict PSII activity while in transit, by using a bicarbonate-mediated switch and protective mechanism.

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Section: Introductionmentioning

confidence: 99%

Bicarbonate Activation of Monomeric Photosystem II-PsbS/Psb27 Complex

Rutherford

Fantuzzi

Piano

et al. 2022

Preprint

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“…However, the NPQ of IG-M remained almost identical to that of SG-Y at all actinic light intensities (Figure 3D). Several previous studies support a PsbS-independent NPQ mechanism (Dall'Osto et al, 2005;Gacek et al, 2019;Johnson & Ruban, 2010). The decrease of PsbS-dependent NPQ in the IG thylakoid was indicated to be compensated in some manner by an increase in PsbS-independent NPQ.…”

Section: Psii-lhcii Organization and Thylakoid Architecturementioning

confidence: 76%

Growth‐phase dependent morphological alteration in higher plant thylakoid is accompanied by changes in both photodamage and repair rates

Nozue

Shigarami

Fukuda

et al. 2021

Physiologia Plantarum

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Thylakoid membranes of young leaves consist of grana and stroma lamellae (stromagrana [SG] structure). The SG thylakoid is gradually converted into isolated grana (IG), almost lacking the stroma lamellae during growth. This morphological alteration was found to cause a reduction in maximum photosynthetic rate and an enhancement of photoinhibition in photosystem II (PSII). In situ microspectrometric measurements of chlorophyll fluorescence in individual chloroplasts suggested an increase of the PSII/ PSI ratio in IG thylakoids of mature leaves. Western blot analysis of isolated IG thylakoids showed relative increases in some PSII components, including the core protein (D1) and light-harvesting components CP24 and Lhcb2. Notably, a nonphotochemical quenching-related factor in the PSII supercomplex, PsbS, decreased by 40%. Changes in the high light response of PSII were detected through parameters of pulseamplitude modulation fluorometry. Chlorophyll fluorescence lifetime indicated an increase of fluorescence quantum yield in IG. A minimal photodamage-repair rate analysis on a lincomycin treatment of the leaves indicated that repair rate constant of IG is slower than that of SG, while photodamage rate of IG is higher than that of SG. These results suggest that IG thylakoids are relatively sensitive to high light, which is not only due to a higher photodamage rate caused by some rearrangements of PS complexes, but also to the retarded PSII repair that may result from the lack of stroma lamellae. The IG thylakoids found among many plant species thus seem to be an adaptive form to low light environments, although their physiological roles still remain unclear.

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“…Despite the availability of a PsbS crystallographic structure ( Fan et al 2015 ), there is still a debate about its basic components. The presence of chlorophyll and xanthophyll ( Correa-Galvis et al 2016 ; Gachek et al 2019 ) and its role as a luminal pH sensor ( Bergantino et al 2003 ; Li et al 2004 ; Roach and Krieger-Liszkay 2012 ; Liguori et al 2019 ) are still debated. The primary role of PsbS is thought to be a protective one, as a key player in some aspects of nonphotochemical quenching (NPQ) ( Niyogi and Truong 2013 ; Ruban 2016 ; Bassi and Dall’Osto 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Bicarbonate activation of the monomeric photosystem II-PsbS/Psb27 complex

et al. 2023

View full text Add to dashboard Cite

In thylakoid membranes, Photosystem II (PSII) monomers from the stromal lamellae contain the subunits PsbS and Psb27 (PSIIm-S/27), while PSII monomers from granal regions (PSIIm) lack these subunits. Here, we have isolated and characterised these two types of Photosystem II complexes in tobacco (Nicotiana tabacum). PSIIm-S/27 showed enhanced fluorescence, the near-absence of oxygen evolution, as well as limited and slow electron transfer from QA to QB compared to the near-normal activities in the granal PSIIm. However, when bicarbonate was added to PSIIm-S/27, water splitting and QA to QB electron transfer rates were comparable to those in granal PSIIm. The findings suggest that the binding of PsbS and/or Psb27 inhibits forward electron transfer and lowers the binding affinity for bicarbonate. This can be rationalized in terms of the recently discovered photoprotection role played by bicarbonate binding via the redox tuning of the QA/QA•– couple, which controls the charge recombination route, and this limits chlorophyll triplet mediated 1O2 formation. These findings suggest that PSIIm-S/27 is an intermediate in the assembly of PSII in which PsbS and/or Psb27 restrict PSII activity while in transit using a bicarbonate-mediated switch and protective mechanism.

show abstract

PsbS‐dependent and ‐independent mechanisms regulate carotenoid‐chlorophyll energy coupling in grana thylakoids

Cited by 4 publications

References 43 publications

Bicarbonate Activation of Monomeric Photosystem II-PsbS/Psb27 Complex

Bicarbonate Activation of Monomeric Photosystem II-PsbS/Psb27 Complex

Growth‐phase dependent morphological alteration in higher plant thylakoid is accompanied by changes in both photodamage and repair rates

Bicarbonate activation of the monomeric photosystem II-PsbS/Psb27 complex

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