Pseudaquidulcibacter saccharophilus gen. nov., sp. nov., a novel member of family Caulobacteraceae, isolated from a water purification facility with supplement of starch as a carbon source

Abstract

During a survey of microbial communities in the influent (ambient water) and effluent of a water purification facility with aeration and supplement of starch as carbon source, a novel bacterial strain, designated SZ9T, was isolated from the effluent sample. Colonies of strain SZ9T were small (approximately 0.5–1.0 mm in diameter), creamy-white, circular, smooth, translucent and convex. Cells were facultative anaerobic, motile by means of a single polar flagellum, rod-shaped, multiplied by binary fission, Gram-stain-negative, oxidase-positive and catalase-negative. Growth occurred at 10–40 °C (optimum, 28 °C) and pH 5.5–8.0 (optimum, pH 7.5). The range of NaCl concentration for growth was 0–1.0 % (w/v), with an optimum of 0–0.5 % (w/v). Phylogenetic analysis based on 16S rRNA gene sequences suggested that strain SZ9T formed a lineage within the family Caulobacteraceae of the class Alphaproteobacteria and showed the highest 16S rRNA gene sequence similarities to Aquidulcibacter paucihalophilus TH1-2T (92.44%), followed by Vitreimonas flagellata SYSU XM001T (89.61 %), Asprobacter aquaticus DRW22-8T (89.49 %) and Hyphobacterium vulgare WM6T (89.49%). The predominant fatty acids (>10 % of the total fatty acids) of strain SZ9T was summed feature 3 (comprising C16 : 1 ω6c and/or C16 : 1 ω7c), summed feature 8 (C18 : 1 ω6c and/or C18 : 1 ω7c) and C16 : 0. The sole respiratory quinone was ubiquinone-10, and the major polar lipids were phosphatidylcholine and two unidentified glycolipids. The whole genome of strain SZ9T was 2 842 140 bp in size, including 2769 protein-coding genes, 37 tRNA genes and two rRNA genes, and the genomic G+C content was 41.4 mol%. The orthologous average nucleotide identity, average amino acid identity and digital DNA–DNA hybridization values between strain SZ9T and other genera within the family Caulobacteraceae were 64.50–66.62 %, 46.96–54.17 % and 27.70–31.70 %, respectively. Therefore, based on the results of phenotypic, chemotaxonomic and phylogenetic analyses, the isolated strain SZ9T could be distinguished from other genera, suggesting that it represents a novel species of a novel genus in the family Caulobacteraceae , for which the name Pseudaquidulcibacter saccharophilus gen. nov., sp. nov is proposed. The type strain is SZ9T (=CCTCC AB2021029T=KCTC 82788T).

Peiella sedimenti gen. nov., sp. nov., a novel taxon within the family Caulobacteraceae isolated from sediment of a river

“…Growth was monitored by measuring OD 600 . Antibiotic sensitivity was tested on R2A agar by the disc method [6]. Other physiological and biochemical features of XZ-24 T were determined using the API ZYM, API 20NE (bioMérieux) and GENIII (Biolog) identification systems according to the instructions of the manufacturers.…”

“…The family Caulobacteraceae in the order Caulobacterales of the class Alphaproteobacteria was proposed by Henrici and Johnson [1]. According to the List of Prokaryotic Names with Standing in Nomenclature (LSPN, https://lpsn.dsmz.de/family/caulobacteraceae), the family Caulobacteraceae currently consists of seven genera with validly published names: Aquidulcibacter [2] , Asticcacaulis [3] , Brevundimonas [4] , Caulobacter [1] , Phenylobacterium [5] , Pseudaquidulcibacter [6] and Terricaulis [7] with Caulobacter as the type genus. Members of the family have been isolated from samples from a variety of environments such as freshwater [8], seawater [9], activated sludge [10], blood [11], rhizosphere [12], soil [13] and cyanobacterial aggregate [2].…”