Pseudo-nitzschia fukuyoi (Bacillariophyceae), a domoic acid-producing species from Nha Phu Bay, Khanh Hoa Province, Vietnam

Forty-eight isolates of Pseudo-nitzschia species were established from the Miri coast of Sarawak (Malaysian Borneo) and underwent TEM observation and molecular characterization. Ten species were found: P. abrensis, P. batesiana, P. fukuyoi, P. kodamae, P. lundholmiae, P. multistriata, P. pungens, P. subfraudulenta, as well as two additional new morphotypes, herein designated as P. bipertita sp. nov. and P. limii sp. nov. This is the first report of P. abrensis, P. batesiana, P. kodamae, P. fukuyoi, and P. lundholmiae in coastal waters of Malaysian Borneo. Pseudo-nitzschia bipertita differs from its congeners by the number of sectors that divide the poroids, densities of band striae, and its cingular band structure. Pseudo-nitzschia limii, a pseudo-cryptic species in the P. pseudodelicatissima complex sensu lato, is distinct by having wider proximal and distal mantles, a higher number of striae, and greater poroid height in the striae of the valvocopula. The species were further supported by the phylogenetic reconstructions of the nuclear-encoded large subunit ribosomal gene and the second internal transcribed spacer. Phylogenetically, P. bipertita clustered with its sister taxa (P. subpacifica + P. heimii); P. limii appears as a sister taxon to P. kodamae and P. hasleana in the ITS2 tree. Pairwise comparison of ITS2 transcripts with its closest relatives revealed the presence of both hemi- and compensatory base changes. Toxicity analysis showed detectable levels of domoic acid in P. abrensis, P. batesiana, P. lundholmiae, and P. subfraudulenta, but both new species tested below the detection limit.

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“…, ) and discoveries of toxigenic Pseudo‐nitzschia species (Dao et al. , , Teng et al. ) continue to be reported from Southeast Asia.…”

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confidence: 98%

High diversity of Pseudo‐nitzschia along the northern coast of Sarawak (Malaysian Borneo), with descriptions of P. bipertita sp. nov. and P. limii sp. nov. (Bacillariophyceae)

Teng

Tan

Lim

et al. 2016

Journal of Phycology

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“…, Dao et al. ). Species in the genus are identified by electron microscopy based on frustule ultrastructure and molecular differences.…”

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confidence: 99%

“…The number of species in the genus has increased abruptly in the past two decades, currently comprising 44. However, not all of the species are harmful; only 19 are known to produce the neurotoxin domoic acid (DA; reviewed in Trainer et al 2012, Dao et al 2015. Species in the genus are identified by electron microscopy based on frustule ultrastructure and molecular differences.…”

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confidence: 99%

A non‐toxigenic but morphologically and phylogenetically distinct new species of Pseudo‐nitzschia, P. sabit sp. nov. (Bacillariophyceae)

Teng

Lim

et al. 2015

Journal of Phycology

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A new species of Pseudo-nitzschia (Bacillariophyceae) is described from plankton samples collected from Port Dickson (Malacca Strait, Malaysia) and Manzanillo Bay (Colima, Mexico). The species possesses a distinctive falcate cell valve, from which they form sickle-like colonies in both environmental samples and cultured strains. Detailed observation of frustules under TEM revealed ultrastructure that closely resembles P. decipiens, yet the new species differs by the valve shape and greater ranges of striae and poroid densities. The species is readily distinguished from the curve-shaped P. subcurvata by the presence of a central interspace. The morphological distinction is further supported by phylogenetic discrimination. We sequenced and analyzed the nuclear ribosomal RNA genes in the LSU and the second internal transcribed spacer, including its secondary structure, to infer the phylogenetic relationship of the new species with its closest relatives. The results revealed a distinct lineage of the new species, forming a sister cluster with its related species, P. decipiens and P. galaxiae, but not with P. subcurvata. We examined the domoic acid (DA) production of five cultured strains from Malaysia by Liquid chromatography-mass spectrometry (LC-MS), but they showed no detectable DA. Here, we present the taxonomic description of the vegetative cells, document the sexual reproduction, and detail the molecular phylogenetics of Pseudo-nitzschia sabit sp. nov.

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“…Since this review, however, many phytoplankton surveys around the globe have revealed further hidden diversity. This veiled diversity was revealed by several researchers (Lundholm et al 2003, ; Amato & Montresor ; Lim et al , ; Orive et al ; Teng et al , ; Dao et al ; Percopo et al ), with one particular study revealing two new species P. calliantha Lundholm, Moestrup and Hasle and P. caciantha Lundholm, Moestrup and Hasle from the re‐examination of specimens previously ascribed to Pseudo‐nitzschia pseudodelicatissima (Hasle) Hasle complex sensu Lundholm et al (2003). Since the first identification of P. caciantha , it has been reported from at least 11 locations in both the northern hemisphere and Southeast Asia, whilst only one from the southern hemisphere (Table ).…”

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confidence: 68%

The first report of the potentially harmful diatom Pseudo‐nitzschia caciantha from Australian coastal waters

Ajani

Kim

Han

et al. 2016

Phycological Research

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SUMMARY The diatom Pseudo‐nitzschia is a significant component of coastal waters worldwide and a producer of the potent neurotoxin, domoic acid. Sixteen species belonging to this genus have been reported from Australian waters, but the potentially toxic species P. caciantha has not been previously known from this region. Two clonal strains of P. caciantha were isolated from Coogee Beach, south‐east Australia, and the morphological, molecular and toxicological evidence for this species delineation were examined using light and transmission electron microscopy, phylogenetic analysis based on sequences of the second internal transcribed spacer and domoic acid production as measured by liquid chromatography–mass spectrometry. The results unambiguously confirmed that these isolates are the potentially toxic species P. caciantha , being only the second report of this species in the Southern Hemisphere. The potential for further hidden Pseudo‐nitzschia diversity in these waters is considerable.

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Pseudo-nitzschia fukuyoi (Bacillariophyceae), a domoic acid-producing species from Nha Phu Bay, Khanh Hoa Province, Vietnam

Cited by 25 publications

References 15 publications

High diversity of Pseudo‐nitzschia along the northern coast of Sarawak (Malaysian Borneo), with descriptions of P. bipertita sp. nov. and P. limii sp. nov. (Bacillariophyceae)

High diversity of Pseudo‐nitzschia along the northern coast of Sarawak (Malaysian Borneo), with descriptions of P. bipertita sp. nov. and P. limii sp. nov. (Bacillariophyceae)

A non‐toxigenic but morphologically and phylogenetically distinct new species of Pseudo‐nitzschia, P. sabit sp. nov. (Bacillariophyceae)

The first report of the potentially harmful diatom Pseudo‐nitzschia caciantha from Australian coastal waters

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