Pseudoalteromonas maricaloris sp. nov., isolated from an Australian sponge, and reclassification of [Pseudoalteromonas aurantia] NCIMB 2033 as Pseudoalteromonas flavipulchra sp. nov.

Abstract

A marine, gram-negative, aerobic bacterium that produced cytotoxic, lemon-yellow, chromopeptide pigments that inhibited the development of sea urchin eggs has been isolated from the Australian sponge Fascaplysinopsis reticulata Hentschel. The cells of the organism were rod-shaped with a single polar flagellum and they required NaCl for growth (0.5-10%) with optimum growth at 1-3% NaCl. The temperature for growth was 10-37 degrees C, with optimum growth at 25-30 degrees C. Growth occurred at pH values from 6.0 to 10.0, with optimum growth at pH 6.0-8.0. Major phospholipids were phosphatidylethanolamine, phosphatidylglycerol and lyso-phosphatidylethanolamine. Of 26 fatty acids with 11-19 carbon atoms that were detected, 16:1omega7, 16:0, 17:1omega8 and 18:1omega7 were predominant. The DNA G+C content was 38.9 mol%. All of these phenotypic and chemotaxonomic characters place the organism in the genus Pseudoalteromonas (Gauthier et al, 1995). These data are consistent with the phylogenetic analyses that confirmed that strain KMM 636T is a member of the Pseudoalteromonas cluster in the gamma-subclass of the Proteobacteria. DNA-DNA hybridization experiments revealed that the levels of relatedness between the DNA of the strain studied and DNAs of type strains of the species that clustered together (on the basis of 16S rDNA sequences) and [Pseudoalteromonas aurantia] NCIMB 2033 ranged from 19 to 35%, and that the DNA-DNA homology between [P. aurantia] NCIMB 2033 and other phylogenetically and/or phenotypically similar type strains ranged from 32 to 52%. According to the polyphasic evidence presented in this study, it is proposed that strain KMM 636T (= LMG 19692T = CIP 106859T) be classified as Pseudoalteromonas maricaloris sp. nov. and [P. aurantia] NCIMB 2033 be reclassified as Pseudoalteromonas flavipulchra NCIMB 2033T (= KMM 3630T = LMG 20361T) sp. nov.

Improved growth performance of the mangrove Avicennia marina seedlings using a 1-aminocyclopropane-1-carboxylic acid deaminase-producing isolate of Pseudoalteromonas maricaloris

“…The most promising ACC deaminase-producing isolate (# 15) was identified as Pseudoalteromonas maricaloris Ivanova et al (2002). Evaluation of plant growth-promoting activities of WT and NPM strains of P. maricaloris…”

Biofilm Development and Cell Death in the Marine Bacterium Pseudoalteromonas tunicata

“…have been isolated from diverse marine habitats globally (13,18,36,49) and are frequently found in association with the surfaces of eukaryotic hosts. Species have been isolated from various animals, such as mussels (23,25), puffer fish (47), tunicates (20), and sponges (26), as well as from a range of marine plants (13,24,56).…”

Antifouling activity of sessile bacilli derived from marine surfaces

“…Antibacterial tests were carried out in duplicate in Petri dishes containing 15 ml of Marine Agar 2216, inoculated with 0.2 ml of a bacterial suspension of H. marina with an OD 520 nm of 0.3 (»2 £ 10 9 cells/ml). The bacterial suspension was homogeneously swabbed on the agar surface and Wve wells of 6 mm diameter were made on the agar using a sterile Pasteur Pipette [15]. The solutions of extracts were prepared at a concentration of 5% in dimethyl sulfoxide (DMSO, w/v; Sigma).…”