Pseudocholinesterase Activity in Human Cerebrospinal Fluid

Kambam,; Horton, Brent M.; Wc, Parris; Sa, Hyman; Ml, Berman; Bv, Sastry

doi:10.1213/00000539-198904000-00011

Cited by 13 publications

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“…Moreover, previous measurements of PChE only in CSF showed high variability (13). This is why we used PChE CSF/serum ratio, presented in percentage, as a variable expected to be unaffected by age, sex, or tumor type/pathological developments.…”

Section: Methodsmentioning

confidence: 98%

“…PChE activity in CSF in healthy people is rarely investigated, most often as a part of studies on specific diseases or anesthetics (11-13). Lumbar puncture is an invasive procedure and for ethical reasons the number of healthy participants in studies is always small, especially in case of children.…”

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confidence: 99%

“…For this reason the information about the normal values of PChE activity in CSF in relation to age at all ages is scarce. In adults, PChE activity in CSF ranges from 1/20 to 1/100 of the value of serum (13). In children, the values are age-dependent (14).…”

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confidence: 99%

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Pseudocholinesterase activity in cerebrospinal fluid as a biomarker of solid central nervous system tumors in children

et al. 2013

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AimTo determine the activity of pseudocholinesterase (PChE) in cerebrospinal fluid (CSF) and serum in children with solid central nervous system (CNS) tumor and to assess whether PChE activity could be a valid biomarker for solid CNS tumors in children.MethodsThe study and control group included 30 children each. Children in the study group had a solid CNS tumor, while those from the control group had never suffered from any tumor diseases. CSF and serum samples were collected from all participants and PChE activity was determined using the Ellman’s spectrophotometric method. PChE activity in CSF was shown as a cerebrospinal fluid/serum ratio expressed in percentage, ie, PChE CSF/serum ratio. Receiver operating characteristic (ROC) curve was used to assess whether PChE activity can be used as a biomarker for identifying children with solid CNS tumors.ResultsChildren with solid CNS tumor had significantly higher PChE activity in CSF and serum, as well as PChE CSF/serum ratio (P = 0.001). PChE CSF/serum ratio in the study group was 2.38% (interquartile range [IQR] 1.14-3.97) and 1.09% (IQR 0.95-1.45) in the control group. ROC curve analysis of PChE CSF/serum ratio resulted in an area under the curve (AUC) value of 0.76 (95% confidence interval [CI] 0.63-0.88) and a cut-off of 1.09. Twenty five of 29 patients with elevated PChE CSF/serum ratio had a tumor, corresponding to a sensitivity of 83% and a specificity of 53%.ConclusionPChE CSF/serum ratio may be used as a test or biomarker with good sensitivity for solid CNS tumors in children.

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Section: Methodsmentioning

confidence: 98%

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confidence: 99%