Pseudomonas aeruginosa biofilm as a diffusion barrier to piperacillin

Hoyle, B.; Alcantara, Joenel; Costerton, J. W.

doi:10.1128/aac.36.9.2054

Cited by 152 publications

(107 citation statements)

References 19 publications

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“…This conclusion is supported by a number of other investigations suggesting that aminoglycosides diffuse more slowly because they bind to extracellular polymers such as alginate (13,14,20). There are conflicting results regarding the penetration of a ␤-lactam antibiotic, piperacillin (15,24).…”

supporting

confidence: 61%

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Contributions of Antibiotic Penetration, Oxygen Limitation, and Low Metabolic Activity to Tolerance of Pseudomonas aeruginosa Biofilms to Ciprofloxacin and Tobramycin

Walters¹,

Roe²,

Bugnicourt³

et al. 2003

Antimicrob Agents Chemother

866

675

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The roles of slow antibiotic penetration, oxygen limitation, and low metabolic activity in the tolerance of Pseudomonas aeruginosa in biofilms to killing by antibiotics were investigated in vitro. Tobramycin and ciprofloxacin penetrated biofilms but failed to effectively kill the bacteria. Bacteria in colony biofilms survived prolonged exposure to either 10 g of tobramycin ml ؊1 or 1.0 g of ciprofloxacin ml ؊1 . After 100 h of antibiotic treatment, during which the colony biofilms were transferred to fresh antibiotic-containing plates every 24 h, the log reduction in viable cell numbers was only 0.49 ؎ 0.18 for tobramycin and 1.42 ؎ 0.03 for ciprofloxacin. Antibiotic permeation through colony biofilms, indicated by a diffusion cell bioassay, demonstrated that there was no acceleration in bacterial killing once the antibiotics penetrated the biofilms. These results suggested that limited antibiotic diffusion is not the primary protective mechanism for these biofilms. Transmission electron microscopic observations of antibiotic-affected cells showed lysed, vacuolated, and elongated cells exclusively near the air interface in antibiotic-treated biofilms, suggesting a role for oxygen limitation in protecting biofilm bacteria from antibiotics. To test this hypothesis, a microelectrode analysis was performed. The results demonstrated that oxygen penetrated 50 to 90 m into the biofilm from the air interface. This oxic zone correlated to the region of the biofilm where an inducible green fluorescent protein was expressed, indicating that this was the active zone of bacterial metabolic activity. These results show that oxygen limitation and low metabolic activity in the interior of the biofilm, not poor antibiotic penetration, are correlated with antibiotic tolerance of this P. aeruginosa biofilm system.

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supporting

confidence: 61%

“…Several articles report measurements of antibiotic penetration in P. aeruginosa biofilms (15,18,24,30,31,35). The general consensus of these studies is that fluoroquinolones, such as ofloxacin and ciprofloxacin, penetrate P. aeruginosa biofilms readily while aminoglycosides, such as tobramycin and gentamicin, are retarded in their delivery.…”

mentioning

confidence: 99%

Contributions of Antibiotic Penetration, Oxygen Limitation, and Low Metabolic Activity to Tolerance of Pseudomonas aeruginosa Biofilms to Ciprofloxacin and Tobramycin

Walters¹,

Roe²,

Bugnicourt³

et al. 2003

Antimicrob Agents Chemother

866

675

View full text Add to dashboard Cite

show abstract

“…This, together with the observation that in vivo SP variants may promote colonisation of implants and consequent resistance to antibiotics [15], may explain the fact that, in patients with prostheses, only 32% of infections caused by SP coagulase-negative staphylococci are eliminated by antibiotics, reaching 100% successful recovery of the patient in infections by NSP strains [9]. Two hypotheses have been put forward to explain this biofilm-associated phenomenon of resistance [8]: (a) antibiotics do not reach bacteria present in the 49 Table 1.…”

Section: Discussionmentioning

confidence: 99%

Adherence of Staphylococcus aureus slime-producing strain variants to biomaterials used in orthopaedic surgery

Gracia¹,

Fernández

Conchello

et al. 1997

International Orthopaedics

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“…These structures assist in the persistence of P. mirabilis in the urinary tract by protecting these organisms from antibiotics and the host immune response and obviously contribute to adhesion to surfaces (146). Urinary stone formation during Proteus-mediated UTI is characteristic of this type of infection and is critical for the development of crystalline biofilms.…”

Section: Biofilm Formationmentioning

confidence: 99%

Complicated Catheter-Associated Urinary Tract Infections Due toEscherichia coliandProteus mirabilis

et al. 2008

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SUMMARY Catheter-associated urinary tract infections (CAUTIs) represent the most common type of nosocomial infection and are a major health concern due to the complications and frequent recurrence. These infections are often caused by Escherichia coli and Proteus mirabilis. Gram-negative bacterial species that cause CAUTIs express a number of virulence factors associated with adhesion, motility, biofilm formation, immunoavoidance, and nutrient acquisition as well as factors that cause damage to the host. These infections can be reduced by limiting catheter usage and ensuring that health care professionals correctly use closed-system Foley catheters. A number of novel approaches such as condom and suprapubic catheters, intermittent catheterization, new surfaces, catheters with antimicrobial agents, and probiotics have thus far met with limited success. While the diagnosis of symptomatic versus asymptomatic CAUTIs may be a contentious issue, it is generally agreed that once a catheterized patient is believed to have a symptomatic urinary tract infection, the catheter is removed if possible due to the high rate of relapse. Research focusing on the pathogenesis of CAUTIs will lead to a better understanding of the disease process and will subsequently lead to the development of new diagnosis, prevention, and treatment options.

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Pseudomonas aeruginosa biofilm as a diffusion barrier to piperacillin

Abstract: Pseudomonas aeruginosa 579 biofilms formed on dialysis membranes retarded piperacillin diffusion.

Cited by 152 publications

References 19 publications

Contributions of Antibiotic Penetration, Oxygen Limitation, and Low Metabolic Activity to Tolerance of Pseudomonas aeruginosa Biofilms to Ciprofloxacin and Tobramycin

Contributions of Antibiotic Penetration, Oxygen Limitation, and Low Metabolic Activity to Tolerance of Pseudomonas aeruginosa Biofilms to Ciprofloxacin and Tobramycin

Adherence of Staphylococcus aureus slime-producing strain variants to biomaterials used in orthopaedic surgery

Complicated Catheter-Associated Urinary Tract Infections Due toEscherichia coliandProteus mirabilis

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