Pseudomonas aeruginosa Biofilms in Disease

Mulcahy, Lawrence; Isabella, Vincent M.; Lewis, Kim

doi:10.1007/s00248-013-0297-x

Cited by 423 publications

(337 citation statements)

References 112 publications

(129 reference statements)

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“…However, as Hsu et al [48] have described, attachment phenomena for bacterial cells at the nanoscale is less understood, as relatively few reported works have explored the effects of nanoscale topography on bacterial attachment behavior and biofilm formation [23,49,54,86,87]. In fact, some work on bacterial attachment to nanoscale topography resulted in a higher degree of attachment to nanoscaled surfaces rather than planar or micron-scaled topography [88][89][90][91]. Other groups, however, have seen an antifouling effect on nanoscaled surfaces [23,92].…”

Section: Pushing Antifouling Topography To the Nanoscalementioning

confidence: 99%

“…Their orthogonal double-gradient fabricated array consisted of a pitch gradient ranging from 0.8 to 4.0 μm, and an orthogonal nanopost diameter gradient from 300 nm to ~1 μm. They performed studies using Pseudomonas aeruginosa [90]. These HAR nano and microscale structures were shown to induce long-range spontaneous spatial patterning of P. aeruginosa cells while on the surface.…”

Section: Pushing Antifouling Topography To the Nanoscalementioning

confidence: 99%

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Nano and Microscale Topographies for the Prevention of Bacterial Surface Fouling

2014

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Bacterial surface fouling is problematic for a wide range of applications and industries, including, but not limited to medical devices (implants, replacement joints, stents, pacemakers), municipal infrastructure (pipes, wastewater treatment), food production (food processing surfaces, processing equipment), and transportation (ship hulls, aircraft fuel tanks). One method to combat bacterial biofouling is to modify the topographical structure of the surface in question, thereby limiting the ability of individual cells to attach to the surface, colonize, and form biofilms. Multiple research groups have demonstrated that micro and nanoscale topographies significantly reduce bacterial biofouling, for both individual cells and bacterial biofilms. Antifouling strategies that utilize engineered topographical surface features with well-defined dimensions and shapes have demonstrated a greater degree of controllable inhibition over initial cell attachment, in comparison to undefined, texturized, or porous surfaces. This review article will explore the various approaches and techniques used by researches, including work from our own group, and the underlying physical properties of these highly structured, engineered micro/nanoscale topographies that significantly impact bacterial surface attachment.

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Section: Pushing Antifouling Topography To the Nanoscalementioning

confidence: 99%

Section: Pushing Antifouling Topography To the Nanoscalementioning

confidence: 99%

Nano and Microscale Topographies for the Prevention of Bacterial Surface Fouling

2014

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“…[1][2][3][4] P. aeruginosa can also colonize a number of medical devices, increasing the spread of bacteria in health care institutions. [5][6][7] Pseudomonas infections, like those caused by many other bacteria found in hospitals, are becoming more difficult to treat. Firstly, the pattern of biofilm growth with this bacteria has a key role in the development of chronic infection.…”

Section: Introductionmentioning

confidence: 99%

Gold-functionalized magnetic nanoparticles restrict growth of Pseudomonas aeruginosa

Niemirowicz¹,

Święcicka²,

Wilczewska³

et al. 2014

IJN

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Superparamagnetic iron oxide nanoparticles (SPIONs) and their derivatives (aminosilane and gold-coated) have been widely investigated in numerous medical applications, including their potential to act as antibacterial drug carriers that may penetrate into bacteria cells and biofilm mass. Pseudomonas aeruginosa is a frequent cause of infection in hospitalized patients, and significant numbers of currently isolated clinical strains are resistant to standard antibiotic therapy. Here we describe the impact of three types of SPIONs on the growth of P. aeruginosa during long-term bacterial culture. Their size, structure, and physicochemical properties were determined using transmission electron microscopy, X-ray diffraction analysis, and Fourier transform infrared spectroscopy. We observed significant inhibition of P. aeruginosa growth in bacterial cultures continued over 96 hours in the presence of gold-functionalized nanoparticles (Fe 3 O 4 @Au). At the 48-hour time point, growth of P. aeruginosa , as assessed by the number of colonies grown from treated samples, showed the highest inhibition (decreased by 40%). These data provide strong evidence that Fe 3 O 4 @Au can dramatically reduce growth of P. aeruginosa and provide a platform for further study of the antibacterial activity of this nanomaterial.

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“…Further, we also examined the in vivo inflammatory effects and systemic silver distribution of implanted AgNP-containing hydrogels in mice to determine the safety of AgNP-containing implants for future clinical application. These materials in a near future could be used as tissue scaffolds for skin lesions such as burns and diabetic ulcers, 22,23 and the inner linings of the heart endocardium associated with implants. 24,25 Experimental…”

Section: Introductionmentioning

confidence: 99%

Safety and efficacy of composite collagen–silver nanoparticle hydrogels as tissue engineering scaffolds

et al. 2015

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The increasing number of multidrug resistant bacteria has revitalized interest in seeking alternative sources for controlling bacterial infection. Silver nanoparticles (AgNPs), are amongst the most promising candidates due to their wide microbial spectrum of action. In this work, we report on the safety and efficacy of the incorporation of collagen coated AgNPs into collagen hydrogels for tissue engineering.The resulting hybrid materials at [AgNPs] < 0.4 µM retained the mechanical properties and biocompatibility for primary human skin fibroblasts and keratinocytes of collagen hydrogels; they also displayed remarkable anti-infective properties against S. aureus, S. epidermidis, E. coli and P. aeruginosa at considerably lower concentrations than silver nitrate. Further, subcutaneous implants of materials containing 0.2 µM AgNPs in mice showed a reduction in the levels of IL-6 and other inflammation markers (CCL24, sTNFR-2, and TIMP1). Finally, an analysis of silver contents in implanted mice showed that silver accumulation primarily occurred within the tissue surrounding the implant. IntroductionBiomaterial-associated infections are a significant healthcare problem and have been linked to medical morbidity and death. [2][3][4][5][6] This has motivated the development of materials with anti-infective properties, such as biomaterials loaded with antibiotics. 7 However, with the increasing number of bacteria that are resistant to antibiotics, 8 silver with historically documented anti-microbial activity has re-gained its attractiveness as an alternative to antibiotics. 9 While toxic to bacteria, it unfortunately is also toxic to mammalian cells. 9,10 More recently, therefore, silver nanoparticles (AgNPs) have been evaluated as a safer alternative to ionic silver. 1,[11][12][13][14][15][16][17][18][19][20] The recent work from our team showed that in comparison with silver, biomolecule-coated, photochemically-produced AgNPs can have both bactericidal and bacteriostatic properties with almost negligible cytotoxic effects. 12 We also showed that oxidation of Ag to AgO is most likely the cause of the cytotoxic effects observed with AgNPs. Our overarching goal is to expand the safe use of AgNPs in the development of implantable hybrid-biomaterials with antiinfective properties for future use as scaffolds to enable regeneration of tissue and organs at a risk of bacterial colonization and concomitant biofilm formation like diabetic foot ulcers. Although some collagen-based materials including † Electronic supplementary information (ESI) available: Representative absorption spectra of AgNP@collagen nanoparticles before and after lyophilization. Absorption spectra for the washes obtained from a 1.0 µM AgNP hydrogel over the course of 5 days. Area under the curve (AUC) calculated from the absorption spectra of 500 µm thickness collagen hydrogels prepared using different concentrations of AgNP@collagen. Selected Cryo-SEM images of BDDGE type I collagen-based hydrogels in the absence or presence of 1.0 µM AgNP. An image of a sel...

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Pseudomonas aeruginosa Biofilms in Disease

Cited by 423 publications

References 112 publications

Nano and Microscale Topographies for the Prevention of Bacterial Surface Fouling

Nano and Microscale Topographies for the Prevention of Bacterial Surface Fouling

Gold-functionalized magnetic nanoparticles restrict growth of Pseudomonas aeruginosa

Safety and efficacy of composite collagen–silver nanoparticle hydrogels as tissue engineering scaffolds

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