Pseudomonas aeruginosa eradicates Staphylococcus aureus by manipulating the host immunity

Pernet, Erwan; Guillemot, Laurent; Burgel, Pierre‐Régis; Martin, Clémence; Lambeau, Gérard; Sermet‐Gaudelus, Isabelle; Sands, Dorota; Leduc, Dominique; Morand, P; Jeammet, Louise; Chignard, Michel; Wu, Yongzheng; Touqui, Lhousseine

doi:10.1038/ncomms6105

Cited by 115 publications

(121 citation statements)

References 71 publications

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“…In a transgenic mouse model, sPLA2-IIA has been shown to protect animals from i.p. or pulmonary infections by S. aureus or Bacillus anthracis, respectively (17)(18)(19). However, these transgenic mice are artificial models with constitutive overexpression of sPLA2-IIA (36).…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, mice are known to exhibit no or very low sPLA2-IIA expression in the lung (37). Therefore, we used guinea pigs as animal model known to produce high pulmonary levels of sPLA2-IIA (18,30). Our results showed that pulmonary infection with the S. aureus DadsA strain, deficient in adenosine production, increased sPLA2-IIA expression in guinea pigs lungs, leading to an enhanced clearance of S. aureus DadsA strain from the airways.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, this enzyme represents a major actor in innate host defense against bacterial infections (12), in particular, by Gram-positive (G + ) bacteria that are highly susceptible to killing by sPLA2-IIA (13)(14)(15)(16). Previous studies showed that constitutively expressed human sPLA2-IIA protected transgenic mice from G + bacterial infections (16)(17)(18)(19).…”

Section: R Espiratory Infections Caused By Staphylococcus Aureusmentioning

confidence: 99%

“…The sPLA2 activity in BALFs from S. aureus-infected guinea pigs was measured, as described previously (18). Briefly, aliquots of BALFs (10 ml) were incubated with substrate ( 3 H-OA-labeled Escherichia coli membrane phospholipids) at 37˚C in assay buffer (0.1 M Tris [pH 7.5], 1 mM CaCl 2 , 0.2% sodium azide).…”

Section: Spla2 Activity Assaymentioning

confidence: 99%

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Staphylococcus aureus Adenosine Inhibits sPLA2-IIA–Mediated Host Killing in the Airways

Pernet

Brunet

Guillemot

et al. 2015

The Journal of Immunology

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Staphylococcus aureus is a common cause of bacterial infections in respiratory diseases. It secretes molecules to dampen host immunity, and the recently identified adenosine is one of these molecules. The type IIA secretory phospholipase A2 (sPLA2-IIA) is a host protein endowed with antibacterial properties, especially against Gram-positive bacteria such as S. aureus. However, the role of adenosine in sPLA2-IIA–mediated S. aureus killing by host is still unknown. The present studies showed that the S. aureus mutant lacking adenosine production (∆adsA strain) increased sPLA2-IIA expression in guinea pig airways and was cleared more efficiently, compared with the wild-type strain. S. aureus ∆adsA strain induced sPLA2-IIA expression by alveolar macrophages after phagocytic process via NOD2–NF-κB–dependent mechanism. However, S. aureus adenosine (wild-type and adsA-complemented strains) and exogenous adenosine downregulated S. aureus phagocytosis by alveolar macrophages, leading to inhibition of sPLA2-IIA expression. This occurred through inhibition of p38 phosphorylation via adenosine receptors A2a-, A2b-, and protein kinase A–dependent pathways. Taken together, our studies suggest that, in the airway, S. aureus escapes sPLA2-IIA–mediated killing through adenosine-mediated inhibition of phagocytosis and sPLA2-IIA expression.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: R Espiratory Infections Caused By Staphylococcus Aureusmentioning

confidence: 99%

Section: Spla2 Activity Assaymentioning

confidence: 99%

See 2 more Smart Citations

Staphylococcus aureus Adenosine Inhibits sPLA2-IIA–Mediated Host Killing in the Airways

Pernet

Brunet

Guillemot

et al. 2015

The Journal of Immunology

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“…Up to now, people have designed many 3D anode structures, such as 3D nanostructures,188, 189 3D porous structures,190, 191, 192, 193 3D network structures,194, 195, 196, 197, 198 etc.…”

Section: Structure Design Of Sn‐based Anode Materialsmentioning

confidence: 99%

Metallic Sn‐Based Anode Materials: Application in High‐Performance Lithium‐Ion and Sodium‐Ion Batteries

2017

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With the fast‐growing demand for green and safe energy sources, rechargeable ion batteries have gradually occupied the major current market of energy storage devices due to their advantages of high capacities, long cycling life, superior rate ability, and so on. Metallic Sn‐based anodes are perceived as one of the most promising alternatives to the conventional graphite anode and have attracted great attention due to the high theoretical capacities of Sn in both lithium‐ion batteries (LIBs) (994 mA h g−1) and sodium‐ion batteries (847 mA h g−1). Though Sony has used Sn–Co–C nanocomposites as its commercial LIB anodes, to develop even better batteries using metallic Sn‐based anodes there are still two main obstacles that must be overcome: poor cycling stability and low coulombic efficiency. In this review, the latest and most outstanding developments in metallic Sn‐based anodes for LIBs and SIBs are summarized. And it covers the modification strategies including size control, alloying, and structure design to effectually improve the electrochemical properties. The superiorities and limitations are analyzed and discussed, aiming to provide an in‐depth understanding of the theoretical works and practical developments of metallic Sn‐based anode materials.

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Heterogeneity Governs 3D‐Cultures of Clinically Relevant Microbial Communities

Peneda Pacheco,

Bertoglio,

Butnarasu

et al. 2023

Adv Funct Materials

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The intrinsic heterogeneity of bacterial niches should be retained in in vitro cultures to represent the complex microbial ecology. As a case study, mucin‐containing hydrogels ‐CF‐Mu3Gel ‐ are generated by diffusion‐induced gelation, bioinspired on cystic fibrosis (CF) mucus, and a microbial niche challenging current therapeutic strategies. At breathing frequency, CF‐Mu3Gel exhibits a G′ and G″ equal to 24 and 3.2 Pa, respectively. Notably, CF‐Mu3Gel exhibits structural gradients with a gradual reduction of oxygen tension across its thickness (280–194 µmol L−1). Over the culture period, a steep decline in oxygen concentration occurs just a few millimeters below the air–mucus interface in CF‐Mu3Gel, similar to those of CF airway mucus. Importantly, the distinctive features of CF‐Mu3Gel significantly influence bacterial organization and antimicrobial tolerance in mono‐ and co‐cultures of Staphylococcus aureus and Pseudomonas aeruginosa that standard cultures are unable to emulate. The antimicrobial susceptibility determined in CF‐Mu3Gel corroborates the mismatch on the efficacy of antimicrobial treatment between planktonically cultured bacteria and those in patients. With this example‐based research, new light is shed on the understanding of how the substrate influences microbial behavior, paving the way for improved fundamental microbiology studies and more effective drug testing and development.

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Pseudomonas aeruginosa eradicates Staphylococcus aureus by manipulating the host immunity

Cited by 115 publications

References 71 publications

Staphylococcus aureus Adenosine Inhibits sPLA2-IIA–Mediated Host Killing in the Airways

Staphylococcus aureus Adenosine Inhibits sPLA2-IIA–Mediated Host Killing in the Airways

Metallic Sn‐Based Anode Materials: Application in High‐Performance Lithium‐Ion and Sodium‐Ion Batteries

Heterogeneity Governs 3D‐Cultures of Clinically Relevant Microbial Communities

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