Pseudomonas aeruginosa: Mannose Sensitive Hemagglutinin Inhibits the Growth of Human Hepatocarcinoma Cells via Mannose-Mediated Apoptosis

Cao, Zhenyuan; Shi, Lijun; Liu, Ying; Wang, Jinghua; Wang, Dandan; Wang, Guangyou; Sun, Bo; Mu, Lili; Yang, Mingfei; Li, Hulun

doi:10.1007/s10620-008-0603-5

Cited by 21 publications

(15 citation statements)

References 29 publications

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“…In recent preclinical studies, cytotoxic effect of PA-MSHA was observed in ER, PR negative breast cancer cells but not in ER, PR positive breast cancer cells [4]. The same effect was also exhibited in human hepatocarcinoma cells treated by PA-MSHA [5]. Given the increasing prevalence of PA-MSHA usage on cancer patients, further laboratory investigation are needed to better understand its anticancer mechanism.…”

Section: Introductionmentioning

confidence: 99%

Inhibition of autophagy enhances the cytotoxic effect of PA-MSHA in breast cancer

et al. 2014

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BackgroundPA-MSHA, a genetically engineered Pseudomonas aeruginosa (PA) strain, is currently under investigation as a new anti-cancer drug. It can induce cell cycle arrest and apoptosis in different human cancer cells, including hormone receptor negative breast cancer cells. However, the underlying mechanism of tumor lethality mediated by PA-MSHA remains to be fully investigated.MethodsThe effect of PA-MSHA on human hormone receptor negative breast cancer cells was analyzed by morphological measurement, western blot, cell proliferation assay and mouse xenograft model.ResultsPA-MSHA was found to induce endoplasmic reticulum (ER) stress in breast cancer cell lines through the IRE1 signaling pathway. Inhibiting autophagy potentiated the cytotoxic effect of PA-MSHA while treating breast cancer cell lines. In mouse xenograft model, PA-MSHA produced more pronounced tumor suppression in mice inoculated with IRE1 gene knockdown. MDA-MB-231HM cells.ConclusionsThese findings demonstrated inhibiting autophagy together with PA-MSHA might be a promising therapeutic strategy in treating hormone receptor negative breast cancer cells.

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Section: Introductionmentioning

confidence: 99%

Inhibition of autophagy enhances the cytotoxic effect of PA-MSHA in breast cancer

et al. 2014

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“…Animal studies have shown that PAMSHA affects both the proinflammatory and anti-inflammatory processes, which help limit the severe adverse reactions caused by systemic inflammation [13]. In other studies, it has been reported to induce apoptosis in tumor cells and improve immune function, which can prevent metastasis and the recurrence of certain types of cancer [14][15][16][17][18][19]. It has also been used to treat malignant pleural effusion [20][21][22].…”

Section: Discussionmentioning

confidence: 99%

Pleurodesis with pseudomonas aeruginosa-mannose–sensitive hemagglutinin for pneumothorax secondary to COPD: a retrospective study

Luo

Tan

et al. 2017

COPD Res Pract

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Background: Pneumothorax is a potentially life-threatening complication of chronic obstructive pulmonary disease (COPD) that leads to cardiopulmonary compromise. According to the British Thoracic Society (BTS) guidelines, medical pleurodesis is recommended for inoperable patients suffering from COPD-related pneumothorax. Several sclerosing agents are currently in use, but none have been proven to be the best choice, as each one has effectiveness and safety issues. Recent research has shown that Pseudomonas aeruginosa-mannose-sensitive hemagglutinin (PAMSHA) is a safe bioagent with low toxicity and good immune-boosting effects that can induce the aseptic inflammation necessary to cause pleural adhesion. The aim of this study is to report our experience using PAMSHA in medical pleurodesis to treat inoperable cases of persistent pneumothorax secondary to COPD. Methods: Records of 78 inoperable patients with persistent pneumothorax secondary to COPD treated with PAMSHA pleurodesis were retrospectively reviewed. Pleurodesis was performed by administering 1 ml of PAMSHA (mixed with lidocaine and 30-40 ml of normal saline) intrapleurally. Results: The resolution of pneumothorax was observed in all of the patients treated with PAMSHA pleurodesis (success rate = 100%). Some of them experienced mild chest pain and fever, but no long-term side effects were reported. Conclusion: Our data suggest that PAMSHA pleurodesis is a safe and effective option for the treatment of persistent pneumothorax secondary to COPD.

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“…PA-MSHA has been used as an adjuvant therapy in the treatment of patients with malignant tumors to enhance immunity, reduce infection rates and improve overall health [12]. However, studies on the direct anti-cancer cytotoxic effects of PA-MSHA are limited: only five studies have demonstrated these effects of PA-MSHA in hepatocarcinoma, gastric cancer, nasopharyngeal cancer, breast cancer and bladder cancer cell lines [11, 13–15]. Therefore, we sought to explore the cytotoxicity of PA-MSHA in cervical cancer cells.…”

Section: Discussionmentioning

confidence: 99%

Pseudomonas aeruginosa mannose-sensitive hemagglutinin inhibits proliferation and invasion via the PTEN/AKT pathway in HeLa cells

et al. 2016

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We investigated the effects of Pseudomonas aeruginosa mannose-sensitive hemagglutinin (PA-MSHA) on the proliferation and invasion of human cervical cancer cell lines, as well as the molecular pathways underlying these effects. MTT cell proliferation assays revealed a time- and concentration-dependent cytotoxic effect of PA-MSHA on HeLa cells but not H8 cells. Flow cytometry with propidium iodide and annexin-V-fluorescein isothiocyanate labeling (FITC) indicated that various concentrations of PA-MSHA could induce apoptosis and G2-M cell cycle arrest in HeLa cells. PA-MSHA also impaired the migration and invasion abilities of HeLa cells in Wound healing and Transwell invasion assays. Western blot results demonstrated that PA-MSHA reduced the expression of p-AKT, p-GSK3β, BCL-2, Vimentin and β-catenin, but increased the levels of PTEN, BAD, BAX and E-cadherin in HeLa cells. Importantly, PTEN siRNA induced the activity of p-AKT, while PA-MSHA partly inhibited this induction, indicating that PA-MSHA may reduce the cell proliferation and invasion potential by activating PTEN and thus inhibiting the AKT pathway in vitro. These data suggest the potential application of PA-MSHA to the treatment of human cervical cancer.

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Pseudomonas aeruginosa: Mannose Sensitive Hemagglutinin Inhibits the Growth of Human Hepatocarcinoma Cells via Mannose-Mediated Apoptosis

Cited by 21 publications

References 29 publications

Inhibition of autophagy enhances the cytotoxic effect of PA-MSHA in breast cancer

Inhibition of autophagy enhances the cytotoxic effect of PA-MSHA in breast cancer

Pleurodesis with pseudomonas aeruginosa-mannose–sensitive hemagglutinin for pneumothorax secondary to COPD: a retrospective study

Pseudomonas aeruginosa mannose-sensitive hemagglutinin inhibits proliferation and invasion via the PTEN/AKT pathway in HeLa cells

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