Pseudomonas aeruginosa Mobbing-Like Behavior against Acanthamoeba castellanii Bacterivore and Its Rapid Control by Quorum Sensing and Environmental Cues

Shteindel, Nimrod; Gerchman, Yoram

doi:10.1128/spectrum.00642-21

Cited by 9 publications

(12 citation statements)

References 40 publications

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“…When comparing different mutants ( Fig. 4 ), results fit our current understanding of P. aeruginosa adhesion behavior– bacteria readily adhere to eukaryotic cells [ 22 , 23 ], using the flagellum for initial adhesion to the surface of the cells [ 24 ]. This causes flagella mutants (Δ flg F and Δ fli C) to display no significant adhesion kinetics ( Fig.…”

Section: Resultsmentioning

confidence: 55%

Quantification of bacterial adhesion to tissue in high-throughput kinetics

Shteindel

Gutman

Atzmon

et al. 2023

Biology Methods and Protocols

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Bacterial adhesion to tissue is the starting point for many pathogenic processes and beneficial interactions. The dynamics and speed of adhesion (minutes) make high-resolution temporal-kinetic data important, but this capability is absent from the current toolset. We present a high-throughput method with a second-to-minute kinetic resolution, testing the adhesion of Pseudomonas aeruginosa PAO1 wild type, flagella-, pili-, and quorum-sensing mutants to human embryonic kidney (HEK293) cells. Adhesion rates were in good correlation with HEK293 confluence, and the ways in which various bacterial mutations modified adhesion patterns are in agreement with the published literature. This simple assay can facilitate drug screening and treatment development as well as provide a better understanding of the interactions of pathogenic and probiotic bacteria with tissues, allowing the design of interventions and prevention treatments.

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Section: Resultsmentioning

confidence: 55%

Quantification of bacterial adhesion to tissue in high-throughput kinetics

Shteindel

Gutman

Atzmon

et al. 2023

Biology Methods and Protocols

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“…2A and B ). According to a previous study, we suggested that the fluorescence results were caused by bacterial attachment, not amoeba phagocytosis ( 24 ). In addition to adhering to amoebae, P. aeruginosa invasion starts with sensing and adhering to host cells ( 25 , 26 ).…”

Section: Resultsmentioning

confidence: 93%

“…Previous research suggests that P. aeruginosa can sense amoebae and attack them by adhering to the amoeba surface ( 19 , 24 ). The images in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…P. aeruginosa isolates that had not adapted to amoebae exhibited chemotaxis and rapidly swam toward the amoebae ( 19 ). A study revealed that coordination of the P. aeruginosa attack against A. castellanii relies on the use of the P. aeruginosa quinolone signal ( 24 ). Based on the available evidence, we propose that the accumulation of P. aeruginosa on amoeba cells amplifies bacterial attachment to the cell barrier that was damaged by amoebae.…”

Section: Discussionmentioning

confidence: 99%

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A. castellanii and P. aeruginosa mutually exacerbate damage to corneal cells during coinfection

Chen,

Liao,

Wang

et al. 2024

Microbiol Spectr

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Microorganisms that are involved in microbial invasion keratitis infiltrate the cornea and cause eye pain. Despite the administration of antimicrobial treatment, patients with amoebic keratitis develop bacterial coinfections. Pseudomonas aeruginosa infection accounts for 50% of the reported bacterial coinfections in these patients. However, the role of pathogenic bacteria in amoeba-induced corneal damage remains unclear. In our current study, we conducted assays to examine cytopathic effects and observed increased corneal cell damage in the group with secondary P. aeruginosa infections. Imaging revealed that the presence of A. castellanii enhanced the accumulation of P. aeruginosa in areas of cell monolayer leakage. We demonstrated that treatment of P. aeruginosa with amoeba-soluble antigen resulted in higher adhesion ability, increased biofilm formation, and more severe corneal cell damage. Overall, this research significantly contributes to our understanding of the risk of P. aeruginosa coinfection in the progression of Acanthamoeba keratitis. IMPORTANCE At the National Cheng Kung University Hospital, numerous cases of amoebic keratitis had been identified with concurrent bacterial infections. Among these bacterial coinfections, Pseudomonas aeruginosa accounted for 50% of the reported cases. However, the impact of pathogenic bacteria on amoeba-induced corneal damage remains unclear. In our study, we successfully demonstrated that P. aeruginosa accumulated on the Acanthamoeba castellanii surface and caused more severe corneal damage. We also indicated that the exposure of P. aeruginosa to amoeba-soluble antigens enhanced its adhesion ability, promoted biofilm formation, and led to more severe corneal cell damage. These findings significantly contributed to our understanding of the risk associated with P. aeruginosa coinfection in the progression of amoeba keratitis.

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“…That is, the expression of the toxin-based bacterial defense is assumed to be jointly controlled by predator recognition and quorum sensing (QS). Examples for such joint control of bacterial defenses have been reported previously [8,26,51]. The involvement of QS in chemical defense strategies is particularly likely as effective toxin concentrations can only be reached when producers are highly abundant.…”

Section: Predator Recognition and Quorum Sensing Interactmentioning

confidence: 97%

Shifts from cooperative to individual-based predation defense determine microbial predator-prey dynamics

et al. 2023

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Predation defense is an important feature of predator-prey interactions adding complexity to ecosystem dynamics. Prey organisms have developed various strategies to escape predation which differ in mode (elude vs. attack), reversibility (inducible vs. permanent), and scope (individual vs. cooperative defenses). While the mechanisms and controls of many singular defenses are well understood, important ecological and evolutionary facets impacting long-term predator-prey dynamics remain underexplored. This pertains especially to trade-offs and interactions between alternative defenses occurring in prey populations evolving under predation pressure. Here, we explored the dynamics of a microbial predator-prey system consisting of bacterivorous flagellates (Poteriospumella lacustris) feeding on Pseudomonas putida. Within five weeks of co-cultivation corresponding to about 35 predator generations, we observed a consistent succession of bacterial defenses in all replicates (n = 16). Initially, bacteria expressed a highly effective cooperative defense based on toxic metabolites, which brought predators close to extinction. This initial strategy, however, was consistently superseded by a second mechanism of predation defense emerging via de novo mutations. Combining experiments with mathematical modeling, we demonstrate how this succession of defenses is driven by the maximization of individual rather than population benefits, highlighting the role of rapid evolution in the breakdown of social cooperation.

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Pseudomonas aeruginosa Mobbing-Like Behavior against Acanthamoeba castellanii Bacterivore and Its Rapid Control by Quorum Sensing and Environmental Cues

Cited by 9 publications

References 40 publications

Quantification of bacterial adhesion to tissue in high-throughput kinetics

Quantification of bacterial adhesion to tissue in high-throughput kinetics

A. castellanii and P. aeruginosa mutually exacerbate damage to corneal cells during coinfection

Shifts from cooperative to individual-based predation defense determine microbial predator-prey dynamics

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