Pseudomonas aeruginosa Promotes Persistence of Stenotrophomonas maltophilia via Increased Adherence to Depolarized Respiratory Epithelium

McDaniel, Melissa S.; Lindgren, Natalie; Billiot, Caitlin E; Valladares, Kristina N; Sumpter, Nicholas A; Swords, W. Edward

doi:10.1128/spectrum.03846-22

Cited by 11 publications

(8 citation statements)

References 54 publications

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“…Production of styrene in the human body associates with an aerobic bacterial metabolism of Pseudomonas aeruginosa [32] mostly found in ears, nose, throat, bovine gut, and on skin [45] . Previous studies have been reported an association between Pseudomonas aeruginosa and several conditions, including pneumonia, enteritis, vaginitis, mastitis, and cirrhosis [46–47] . This pathogenic bacterium has the ability to release different microbial VOCs, and toxins leading to cell membranes degrading when the bacterium works together with cytochrome enzyme(CYP) [25,29] .…”

Section: Resultsmentioning

confidence: 99%

“…[45] Previous studies have been reported an association between Pseudomonas aeruginosa and several conditions, including pneumonia, enteritis, vaginitis, mastitis, and cirrhosis. [46][47] This pathogenic bacterium has the ability to release different microbial VOCs, and toxins leading to cell membranes degrading when the bacterium works together with cytochrome enzyme(CYP). [25,29] In addition, benzene intaking in the human body by inhalation is a substrate for styrene production mostly found in breath and skin as microbial metabolite.…”

Section: Staphylococcus Aureusmentioning

confidence: 99%

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Identification of Volatile Markers in Sweat for COVID‐19 Screening by Gas Chromatography‐Mass Spectrometry

Chuachaina,

Thaveesangsakulthai,

Sinsukudomchai

et al. 2024

ChemistrySelect

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Due to an outbreak of coronavirus spreading in recent years, there is an urgent need for effective, rapid, and non‐invasive diagnostics to detect and differentiate among patients who are infected and uninfected with SARS‐CoV‐2 for controlling the virus propagation. This study developed an alternative method for COVID‐19 screening from volatile analysis of metabolites mainly studied in sweat samples obtained from the populations in Bangkok, Thailand, during December 2020 to April 2022. A total of 140 collected samples; 70 positive confirmed COVID‐19 cases and another 70 negatives confirmed COVID‐19 cases were applied to be separated, detected, and identified as volatile organic compounds (VOCs)/odor occurring from SARS‐CoV‐2 infection body by the technique of Headspace solid‐phase microextraction gas chromatography‐mass spectrometry (HS‐SPME‐GC‐MS) together with principle component analysis (PCA), which allowed potential volatile markers of COVID‐19 such as pentadecane, nonanal, styrene, xylene, ethylbenzene, and 2‐ethylhexyl acrylate (up to 94 % accuracy, 93 % sensitivity, 96 % selectivity, and 94 % specificity), respectively, along with limit of detection in a range of 0.26–125 μg. The result was validated by comparison with the RT‐PCR results.

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Section: Resultsmentioning

confidence: 99%

Section: Staphylococcus Aureusmentioning

confidence: 99%

Identification of Volatile Markers in Sweat for COVID‐19 Screening by Gas Chromatography‐Mass Spectrometry

Chuachaina,

Thaveesangsakulthai,

Sinsukudomchai

et al. 2024

ChemistrySelect

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“…These two pathogens can form a polymicrobial biofilm in the lungs, interact through quorum-sensing signals and create a favourable environment for each other (47). McDaniel et al (48) found that the presence of P. aeruginosa facilitated S. maltophilia persistence in the lungs during polymicrobial infections.…”

Section: Discussionmentioning

confidence: 99%

Management of ventilator‑associated pneumonia due to Stenotrophomonas maltophilia infection: A case report and literature review

et al. 2023

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Stenotrophomonas maltophilia (S. maltophilia) is a difficult-to-treat bacteria. This glucose non-fermenting, multidrug-resistant Gram-negative bacillus is known to cause a range of infections, such as skin manifestations and bacteraemia, with respiratory tract infections being the most common. Patients in intensive care units are often exposed to a higher risk of acquiring an infection caused by this pathogen. Although distinguishing between S. maltophilia infections and colonization can be challenging, pneumonia caused by S. maltophilia infection is becoming increasingly prevalent. Furthermore, S. maltophilia infections are often coupled with Pseudomonas aeruginosa infections, reinforcing the need to consider this bacterium as a potential cause of ventilator-associated pneumonia (VAP). The present study describes the case of a 66-year-old male who was diagnosed with VAP due to S. maltophilia infection. This condition was effectively treated with trimethoprim/sulfamethoxazole. The case described herein underscores the importance of prompt recognition through heightened clinical suspicion, particularly in patients with VAP who are unresponsive to broad-spectrum β-lactam antibiotics. The early identification of S. maltophilia as the root cause of respiratory tract infections can make a significant difference to the outcomes of affected patients.

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“…In the study, LHL-1 and LHL-37 both exhibited a moderate ability to form biofilms and virulence profiles were also identical. The contained virulence factors were primarily contributed to the biofilm formation and colonization in infection [ 31 ]. Infection caused by P. aeruginosa with certain biofilm capacity poses a threat to clinical treatment.…”

Section: Discussionmentioning

confidence: 99%

Emergence and clonal dissemination of KPC-3-producing Pseudomonas aeruginosa in China with an IncP-2 megaplasmid

Qiao

Zheng

et al. 2023

Ann Clin Microbiol Antimicrob

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Background Despite the global prevalence of Klebsiella pneumoniae Carbapenemase (KPC)-type class A β-lactamases, occurrences of KPC-3-producing isolates in China remain infrequent. This study aims to explore the emergence, antibiotic resistance profiles, and plasmid characteristics of blaKPC-3-carrying Pseudomonas aeruginosa. Methods Species identification was performed by MALDI-TOF-MS, and antimicrobial resistance genes (ARGs) were identified by polymerase chain reaction (PCR). The characteristics of the target strain were detected by whole-genome sequencing (WGS) and antimicrobial susceptibility testing (AST). Plasmids were analyzed by S1-nuclease pulsed-field gel electrophoresis(S1-PFGE), Southern blotting and transconjugation experiment. Results Five P. aeruginosa strains carrying blaKPC-3 were isolated from two Chinese patients without a history of travelling to endemic areas. All strains belonged to the novel sequence type ST1076. The blaKPC-3 was carried on a 395-kb IncP-2 megaplasmid with a conserved structure (IS6100-ISKpn27-blaKPC-3-ISKpn6-korC-klcA), and this genetic sequence was identical to many plasmid-encoded KPC of Pseudomonas species. By further analyzing the genetic context, it was supposed that the original of blaKPC-3 in our work was a series of mutation of blaKPC-2. Conclusions The emergence of a multidrug resistance IncP-2 megaplasmid and clonal transmission of blaKPC-3-producing P. aeruginosa in China underlined the crucial need for continuous monitoring of blaKPC-3 for prevention and control of its further dissemination in China.

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Pseudomonas aeruginosa Promotes Persistence of Stenotrophomonas maltophilia via Increased Adherence to Depolarized Respiratory Epithelium

Cited by 11 publications

References 54 publications

Identification of Volatile Markers in Sweat for COVID‐19 Screening by Gas Chromatography‐Mass Spectrometry

Identification of Volatile Markers in Sweat for COVID‐19 Screening by Gas Chromatography‐Mass Spectrometry

Management of ventilator‑associated pneumonia due to Stenotrophomonas maltophilia infection: A case report and literature review

Emergence and clonal dissemination of KPC-3-producing Pseudomonas aeruginosa in China with an IncP-2 megaplasmid

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