Pseudomonas alcaliphila sp. nov., a novel facultatively psychrophilic alkaliphile isolated from seawater.

“…Analyses of whole-cell fatty acids and isoprenoid quinones were performed as described previously (Yumoto et al, 2001(Yumoto et al, , 2002. Analysis of cell wall amino acids was performed using the methods of Schleifer & Kandler (1972).…”

mentioning

confidence: 99%

“…Analyses of whole-cell fatty acids and isoprenoid quinones were performed as described previously (Yumoto et al, 2001(Yumoto et al, , 2002 , 1978) was amplified by PCR. The PCR product (about 1?5 kb) was sequenced directly by the dideoxynucleotide chain-termination method using a DNA sequencer (PRISM 377; Applied Biosystems).…”

mentioning

confidence: 99%

Exiguobacterium oxidotolerans sp. nov., a novel alkaliphile exhibiting high catalase activity

Yumoto

Hishinuma-Narisawa

Hirota

et al. 2004

International Journal of Systematic and Evolutionary Microbiology

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A novel alkaliphile was isolated from a drain of a fish processing plant. The isolate grew at a pH range of 7-10. Cells were Gram-positive, facultatively aerobic, motile rods with peritrichous flagella. Colonies were orange or yellow in colour. Catalase and oxidase reactions were positive. The isolate grew in 0-12 % NaCl but not above 15 % NaCl. Its cell extract exhibited 567 times higher catalase activity than an Escherichia coli cell extract. The major cellular fatty acids were iso-C 13 : 0 , anteiso-C 13 : 0 , iso-C 15 : 0 , iso-C 16 : 0 , iso-C 17 : 0 , anteiso-C 17 : 0 and iso-C 17 : 1 . Its DNA G+C content was 46?7 mol%. Phylogenetic analysis based on 16S rRNA gene sequencing and chemotaxonomic data indicated that strain T-2-2 T is a member of the genus Exiguobacterium. DNA-DNA hybridization revealed a low relatedness of the isolate to several phylogenetic neighbours (less than 25 %). On the basis of phenotypic characteristics, phylogenetic data and DNA-DNA relatedness data, the isolate merits classification as a novel species, for which the name Exiguobacterium oxidotolerans sp. nov. is proposed. The type strain is T-2-2 T (=JCM 12280 T =NCIMB 13980 T ).There are micro-organisms living in environments of extreme temperature, pH, salinity and hydropressure (Horikoshi & Grant, 1991). These micro-organisms have apparently acquired the ability to survive under such environmental conditions through long-term evolutionary processes, and they possess specific mechanisms for survival in such extreme environments. Among such adaptational processes, not only the micro-organisms themselves might be affected by environmental conditions and induced to acquire adaptation-suitable features, but so too might production of organic molecules within the micro-organisms, such as enzymes and proteins that sustain their metabolism. Although H 2 O 2 production and interaction with micro-organisms often occur in nature (Haas & Goebel, 1992), there have been only a few reports of specific micro-organisms that inhabit environments with hyperoxidative stress caused by factors such as high H 2 O 2 concentrations (Yumoto et al., 1999).Aerobic organisms normally possess specific enzymes to eliminate H 2 O 2 , which are produced extracellularly as well as intracellularly. Catalase is one such well-known enzyme. It has been reported that catalase plays an important role in certain micro-organisms in obtaining niches in host cells. For example, in Vibrio fischeri, catalase KatA is required for its colonization of the light organ of squid (Visick & Ruby, 1998). The production of catalase and H 2 O 2 has been reported in several other cases of either parasitic or symbiotic relationships between microorganisms and their host (Katsuwon & Anderson, 1992;Rocha et al., 1996). If there is a micro-organism that is able to survive in an environment with high H 2 O 2 concentrations, it can be expected that this micro-organism will possess an enzyme (e.g. catalase) exhibiting a high H 2 O 2 -degrading ability. In the present study, we isolated a mic...

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“…is relatively small compared to terrestrial Pseudomonas sp. Within Pseudomonas (sensu stricto), the marine isolates include P. stutzeri, formerly P. perfectomarina [7,61], P. alcaligenes, P. pseudoalcaligenes [53], P. Xuorescens [51], P. alcaliphila [80], P. aeruginosa [44], P. xanthomarina [59], P. pachastrellae [58], and P. marincola [57].…”

Section: Phylogenetic Relationship Of Marine Isolates Of Pseudomonasmentioning

confidence: 99%

Bioactive substances produced by marine isolates of Pseudomonas

Isnansetyo

Kamei

2009

J Ind Microbiol Biotechnol

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Pseudomonas is a genus of non-fermentative gram-negative Gammaproteobacteria found both on land and in the water. Many terrestrial isolates of this genus have been studied extensively. While many produce bioactive substances, enzymes, and biosurfactants, other Pseudomonas isolates are used for biological control of plant diseases and bioremediation. In contrast, only a few marine isolates of this genus have been described that produce novel bioactive substances. The chemical structures of the bioactive substances from marine Pseudomonas are diverse, including pyroles, pseudopeptide pyrrolidinedione, phloroglucinol, phenazine, benzaldehyde, quinoline, quinolone, phenanthren, phthalate, andrimid, moiramides, zafrin and bushrin. Some of these bioactive compounds are antimicrobial agents, and dibutyl phthalate and di-(2-ethylhexyl) phthalate have been reported to be cathepsin B inhibitors. In addition to being heterogeneous in terms of their structures, the antibacterial substances produced by Pseudomonas also have diverse mechanisms of action: some affect the bacterial cell membrane, causing bacterial cell lysis, whereas others act as acetyl-CoA carboxylase and nitrous oxide synthesis inhibitors. Marine Pseudomonas spp. have been isolated from a wide range of marine environments and are a potential untapped source for medically relevant bioactive substances.

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Pseudomonas alcaliphila sp. nov., a novel facultatively psychrophilic alkaliphile isolated from seawater.

Cited by 135 publications

References 37 publications

Bacillus oshimensis sp. nov., a moderately halophilic, non-motile alkaliphile

Bacillus oshimensis sp. nov., a moderately halophilic, non-motile alkaliphile

Exiguobacterium oxidotolerans sp. nov., a novel alkaliphile exhibiting high catalase activity

Bioactive substances produced by marine isolates of Pseudomonas

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