Pseudomonas argentinensis sp. nov., a novel yellow pigment-producing bacterial species, isolated from rhizospheric soil in Córdoba, Argentina

Abstract

Microbiología Agrícola, Facultad de Ciencias Agropecuarias, Universidad Nacional de Có rdoba, ArgentinaDuring a study in the Argentinian region of Chaco (Có rdoba), some strains were isolated from the rhizosphere of grasses growing in semi-desertic arid soils. Two of these strains, one isolated from the rhizospheric soil of Chloris ciliata (strain CH01 T ) and the other from Pappophorum caespitosum (strain PA01), were Gram-negative, strictly aerobic rods, which formed yellow round colonies on nutrient agar. They produced a water-insoluble yellow pigment, and a fluorescent pigment was also detected. A polyphasic taxonomic approach was used to characterize the strains. Comparison of the 16S rRNA gene sequences showed a similarity of 99?3 % between them, and phylogenetic analysis revealed that the strains belong to the genus Pseudomonas, within the c-subclass of the Proteobacteria. The closest related species is Pseudomonas straminea IAM 1598 T (similarity of 99?0 % to strain CH01 T and 98?8 % to strain PA01), clustering in a separate branch with the various methods of tree building used. Strains CH01 T and PA01 both had a single polar flagellum, like other yellow pigment-producing pseudomonads related to them. Both strains produced catalase and oxidase. Similar to P. straminea, they did not hydrolyse gelatin or casein. The G+C DNA contents determined were 57?5 mol% for CH01 T and 58?0 mol% for PA01. DNA-DNA hybridization results showed 81 % relatedness between them, and only 40-44 % relatedness with respect to the type strain of P. straminea. These results, together with other phenotypic characteristics, support the conclusion that both isolates belong to the same species, and should be described as representing a novel species within the genus Pseudomonas, for which the name Pseudomonas argentinensis sp. nov. is proposed. The type strain is CH01 T (=LMG 22563 T =CECT 7010 T ).

Fungal and Bacterial Pigments: Secondary Metabolites with Wide Applications

“…However, compared with fungal pigments, most of bacterial pigments are still at the research and development stage ( Table 2 ); hence, work on bacterial pigments production should be intensified to make them available on the market. Pigment producing bacteria are ubiquitous and present in various ecological niches, such as soil (Zhu et al, 2007), rhizospheric soil (Peix et al, 2005), desert sand (Liu et al, 2009), fresh water (Asker et al, 2008), and marine samples (Franks et al, 2005). They were reported in low (Nakamura et al, 2003) and high (Manachini et al, 1985) temperature regions, can persist in salt regions (Asker and Ohta, 1999), and even as endophytes (Deng et al, 2011).…”

Identification of fungal metabolites from inside Gallus gallus domesticus eggshells by non-invasively detecting volatile organic compounds (VOCs)

“…Pseudomonas argentinensis was first reported in 2005 and was found in grasses from Córdoba, Argentina (37). However, it has also been identified in a hog farm sprayed field (North Carolina, USA) (38), in animals (cats from Grenada, West Indies) (39), and in humans as a skin infection (Jerusalem, Palestine) (40).…”

Draft Genome Sequence of the Phosphate-Solubilizing Bacterium Pseudomonas argentinensis Strain SA190 Isolated from the Desert Plant Indigofera argentea

“…Plants were collected in the Jizan area (16°56.475′N, 42°36.694′E) of Saudi Arabia. Based on the 16S rRNA gene sequences, the strain SA190 is closely related (99% gene similarity) to P. argentinensis strain CH01 (NR_043115.1) and P. argentinensis strain PA01 (AY691189.2) (6). …”