Pseudomonas baetica sp. nov., a fish pathogen isolated from wedge sole, Dicologlossa cuneata (Moreau)

López, José R.; Diéguez, Ana L.; Doce, Alejandra; Roca, Elena de la; Herrán, Roberto de la; Navas, J.I.; Toranzo, Alicia E.; Romalde, Jesús L.

doi:10.1099/ijs.0.030601-0

Cited by 59 publications

(44 citation statements)

References 35 publications

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“…() indicated that P. anguilliseptica was more of an opportunistic pathogen in turbot. Pseudomonas baetica was described as a bacterium causing disease of farmed wedge sole, Dicologlossa cuneate (Moreau) (Lopez et al., ). Pseudomonas fluorescens is associated with disease in numerous fish species; it has been isolated from rainbow trout, Oncorhynchus mykiss (Walbaum) with systemic disease (Sakai, Atsuta, & Kobayashi, ), it is an opportunistic pathogen in goldfish, Carassius auratus (Linnaeus) (Bullock, ), and the bacterium caused massive mortality of silver carp, Hypophthalmichthys molitrix (Valenciennes), and bighead carp, Hypophthalmichthys nobilis (Richardson) (Markovic, Radojicic, Snezana, & Levnaic, ).…”

Section: Discussionmentioning

confidence: 99%

A bacterium with close genetic identity to Pseudomonas mandelii associated with spring fish kills in wild bluegill Lepomis macrochirus Rafinesque and pumpkinseed sunfish Lepomis gibbosus (Linnaeus)

Lovy¹,

Dicarlo-Emery

Hutcheson³

2017

Journal of Fish Diseases

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Pseudomonas fluorescens are known bacterial pathogens in fish. The P. fluorescens group contains at least nine different bacterial species, although species from fish have rarely been differentiated. Two isolated fish kills affecting wild bluegills, Lepomis macrochirus Rafinesque, and pumpkinseed sunfish, Lepomis gibbosus (Linnaeus), occurred in the spring of 2015 during cool water temperatures (12.5°C-15.5°C). Disease signs included severe bacteraemia with rare gross external signs. Pure bacterial cultures isolated from kidneys of all affected fish were identified as P. fluorescens using the API 20NE system, while no bacteria were isolated from asymptomatic fish. To further identify the species of bacterium within the P. fluorescens complex, genetic analysis of the 16S rRNA, rpoD and gyrB genes was conducted. DNA sequences of bacterial isolates from both mortality events were identical and had close identity (≥99.7%) to Pseudomonas mandelii. Although likely widespread in the aquatic environment, this is the first report of a bacterium closely resembling P. mandelii infecting and causing disease in fish. The bacterium grew at temperatures between 5°C and 30°C, but not at 37°C. It is possible that infections in fish were a result of immunosuppression associated with spring conditions combined with the psychrotrophic nature of the bacterium.

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Section: Discussionmentioning

confidence: 99%

A bacterium with close genetic identity to Pseudomonas mandelii associated with spring fish kills in wild bluegill Lepomis macrochirus Rafinesque and pumpkinseed sunfish Lepomis gibbosus (Linnaeus)

Lovy¹,

Dicarlo-Emery

Hutcheson³

2017

Journal of Fish Diseases

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“…Recently, Lopez et al . () reported a novel species of P. baetica LMG 25716 T within the group of P. fluorescens with high similarity of 99.4–99.3% with type strains. Although in this study, the genotypes of G and H exhibited high similarity index of 98.0–99.2% to closely related type strains, they formed an outward clade in all phylogenetic analysis and functionally these genotypes exhibited unique properties.…”

Section: Discussionmentioning

confidence: 99%

Novel Phl-producing genotypes of finger millet rhizosphere associated pseudomonads and assessment of their functional and genetic diversity

Sekar

Prabavathy

2014

FEMS Microbiol Ecol

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Genetic diversity of phlD gene, an essential gene in the biosynthesis of 2,4-diacetylphloroglucinol, was studied by restriction fragment length polymorphism (RFLP) in 20 Phl-producing pseudomonads isolated from finger millet rhizosphere. RFLP analysis of phlD gene displayed three patterns with HaeIII and TaqI enzymes. phlD gene sequence closely correlated with RFLP results and revealed the existence of three new genotypes G, H and I. Further, the phylogenetic and concatenated sequence analysis of the 16S rRNA, rpoB, gyrB, rpoD genes supported the hypothesis that these genotypes G, H and I were different from reported genotypes A-F. In all phylogenetic studies, the genotype G formed a distant clade from the groups of Pseudomonas putida and P. aeruginosa (sensu strictu), but the groups H and I were closely related to P. aeruginosa/P. stutzeri group. The Phl-producing pseudomonads exhibited antagonistic activity against Pyricularia grisea (TN508), Gaeumannomyces graminis (DSM1463), Fusarium oxysporum (DSM62297), Xanthomonas campestris (DSM3586) and Erwinia persicina (HMGU155). In addition, these strains exhibited various plant growth-promoting traits. In conclusion, this study displays the existence of novel Phl-producing pseudomonads genotypes G, H and I from finger millet rhizosphere, which formed taxonomically outward phylogenetic lineage from the groups of P. putida and P. aeruginosa (sensu strictu).

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“…Data for other type strains were obtained from references. a, (Chang et al, 2016); b, (Pascual et al, 2015); c, (Tvrzova et al, 2006); d, (Lopez et al, 2012); and e, (Camara et al, 2007). Values are percentages of total fatty acids.…”

Section: Resultsmentioning

confidence: 99%

Pseudomonas glycinae sp. nov. isolated from the soybean rhizosphere

et al. 2020

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Strains MS586T and MS82, which are aerobic, Gram‐negative, rod‐shaped, and polar‐flagellated bacteria, were isolated from the soybean rhizosphere in Mississippi. Taxonomic positions of MS586T and MS82 were determined using a polyphasic approach. 16S rRNA gene sequence analyses of the two strains showed high pairwise sequence similarities (>98%) to some Pseudomonas species. Analysis of the concatenated 16S rRNA, rpoB, rpoD, and gyrB gene sequences indicated that the strains belonging to the Pseudomonas koreensis subgroup (SG) shared the highest similarity with Pseudomonas kribbensis strain 46‐2T. Analyses of average nucleotide identity (ANI), genome‐to‐genome distance, delineated MS586T and MS82 from other species within the genus Pseudomonas. The predominant quinone system of the strain was ubiquinone 9 (Q‐9), and the DNA G+C content was 60.48 mol%. The major fatty acids were C16:0, C17:0 cyclo, and the summed features 3 and 8 consisting of C16:1ω7c/C16:1ω6c and C18:1ω7c/C18:1ω6c, respectively. The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, and diphosphatidylglycerol. Based on these data, it is proposed that strains MS586T and MS82 represent a novel species within the genus Pseudomonas. The proposed name for the new species is Pseudomonas glycinae, and the type strain is MS586T (accession NRRL B‐65441 = accession LMG 30275).

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Pseudomonas baetica sp. nov., a fish pathogen isolated from wedge sole, Dicologlossa cuneata (Moreau)

Cited by 59 publications

References 35 publications

A bacterium with close genetic identity to Pseudomonas mandelii associated with spring fish kills in wild bluegill Lepomis macrochirus Rafinesque and pumpkinseed sunfish Lepomis gibbosus (Linnaeus)

A bacterium with close genetic identity to Pseudomonas mandelii associated with spring fish kills in wild bluegill Lepomis macrochirus Rafinesque and pumpkinseed sunfish Lepomis gibbosus (Linnaeus)

Novel Phl-producing genotypes of finger millet rhizosphere associated pseudomonads and assessment of their functional and genetic diversity

Pseudomonas glycinae sp. nov. isolated from the soybean rhizosphere

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