Pseudomonas fluorescens Pf-5 genome-wide mutant screen for resistance to the antimicrobial peptide alfalfa snakin-1

Ayub, Nicolás Daniel; Fox, Ana Romina; García, Araceli Nora; Mozzicafreddo, Matteo; Cuccioloni, Massimiliano; Angeletti, Mauro; Pagano, Elba; Soto, Gabriela

doi:10.1093/femsle/fnu006

Cited by 11 publications

(6 citation statements)

References 38 publications

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“…A high percentage of the plant-inducible genes (42%) are not shared between the strains, suggesting specialized functions (Silby et al, 2009 ). These strains also possess many genetic determinants for biofilm formation including the pathways controlled by nucleotide second messenger signaling (Gal et al, 2003 ; Paulsen et al, 2005 ; Spiers and Rainey, 2005 ; Koza et al, 2009 ; Silby et al, 2009 ; Kimbrel et al, 2010 ; Newell et al, 2011 ; Boyd et al, 2012 ; Varivarn et al, 2013 ; Martins et al, 2014 ; Ayub et al, 2015 ; Hengge et al, 2016 ). Knowledge about quorum sensing (QS) and auto-inducer pheromone molecule remains sparse for these strains as SBW25 does not produce an AHL-type of auto-inducer (Unge and Jansson, 2001 ; de Bruijn and Raaijmakers, 2009 ; Martins et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

Dynamics of Aspen Roots Colonization by Pseudomonads Reveals Strain-Specific and Mycorrhizal-Specific Patterns of Biofilm Formation

et al. 2018

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Rhizosphere-associated Pseudomonas fluorescens are known plant growth promoting (PGP) and mycorrhizal helper bacteria (MHB) of many plants and ectomycorrhizal fungi. We investigated the spatial and temporal dynamics of colonization of mycorrhizal and non-mycorrhizal Aspen seedlings roots by the P. fluorescens strains SBW25, WH6, Pf0-1, and the P. protegens strain Pf-5. Seedlings were grown in laboratory vertical plates systems, inoculated with a fluorescently labeled Pseudomonas strain, and root colonization was monitored over a period of 5 weeks. We observed unexpected diversity of bacterial assemblies on seedling roots that changed over time and were strongly affected by root mycorrhization. P. fluorescens SBW25 and WH6 stains developed highly structured biofilms with internal void spaces forming channels. On mycorrhizal roots bacteria appeared encased in a mucilaginous substance in which they aligned side by side in parallel arrangements. The different phenotypic classes of bacterial assemblies observed for the four Pseudomonas strains were summarized in a single model describing transitions between phenotypic classes. Our findings also reveal that bacterial assembly phenotypes are driven by interactions with mucilaginous materials present at roots.

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Section: Introductionmentioning

confidence: 99%

Dynamics of Aspen Roots Colonization by Pseudomonads Reveals Strain-Specific and Mycorrhizal-Specific Patterns of Biofilm Formation

et al. 2018

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“…It was also demonstrated that the GRPs have antimicrobial properties and are able to inhibit the growth of various bacterial and fungal pathogens ( 42 , 43 ). In addition, the GRPs may be involved in the interactions with foreign proteins, such as bacterial adhesion protein from Pseudomaonas fluorescense ( 44 ), or interact with plant sucrose transporter SUT-1 ( 45 ). Generally, these proteins are implicated in many biological functions in plants, however, the molecular mechanism of their functions is still not understood.…”

Section: Discussionmentioning

confidence: 99%

When the Frequencies of Sensitization and Elicitation of Allergic Reaction Do Not Correlate—The Case of Apple Gibberellin-Regulated Protein Tested in an Italian Population

Tuppo

Alessandri²,

Giangrieco

et al. 2021

Front. Allergy

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Background: The literature reports describing allergic symptoms against apples in the patients sensitized to the gibberellin-regulated proteins (GRPs) suggested the presence of an allergenic GRP in this fruit.Objective: This study aimed to assess the presence of a GRP protein in apples and investigate its allergenicity.Methods: The protein was isolated and identified by the classical biochemical methods. The bioinformatics tools were used for similar searches and molecular modeling. The immunological features were investigated using the multiplex FABER test. Clinical data were collected by the allergy specialists.Results: A GRP was detected in the apple peel and pulp and it was named applemaclein. This protein displays 94% of sequence identity with peamaclein, Pru p 7, representing the prototype of this allergen family. The applemaclein molecular model shows a very irregular surface with grooves/clefts that may potentially accommodate small molecular ligands. In a population of 4,721 patients in Italy, 187 (4.0%) were sensitized to any allergenic GPR. Of those, 115 (61.5%), 61 (32.6%), 30 (16.0%), and 99 (52.9%) had immunoglobulin E (IgE) to apple, peach, pomegranate, and cypress GRP, respectively. However, in a cohort of the patients in Italy, most individuals IgE positive to the apple GRP did not report allergic reactions against this fruit.Conclusion: Compared with the peach Pru p 7, applemaclein shows some different structural features and higher sensitization frequency, which is often not associated with allergic reactions against apple. Further studies are needed to understand a possible correlation between the applemaclein structural properties, the interaction with still unknown molecules, and immunological behavior.

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“…This approach was adopted to elucidate the molecular mechanism of alfalfa snakin-1 (MsSN1), an antimicrobial peptide produced by Medicago sativa , against Pseudomonas fluorescens Pf-5. This work revealed that MsSN1 peptide acts on adhesion properties of P. fluorescens (Ayub et al, 2015 ; Table 1 ).…”

Section: Genomics and Advances In Antibiotics Researchmentioning

confidence: 91%

Application of Omics Technologies for Evaluation of Antibacterial Mechanisms of Action of Plant-Derived Products

Santos

Silva

et al. 2016

Front. Microbiol.

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In the face of increasing bacterial resistance to antibiotics currently in use, the search for new antimicrobial agents has received a boost in recent years, with natural products playing an important role in this field. In fact, several methods have been proposed to investigate the antibacterial activities of natural products. However, given that the ultimate aim is future therapeutic use as novel drugs, it is extremely necessary to elucidate their modes of action, stating the molecular effects in detail, and identifying their targets in the bacterial cell. This review analyzes the application of “omics technologies” to understand the antibacterial mechanisms of bioactive natural products, to stimulate research interest in this area and promote scientific collaborations. Some studies have been specifically highlighted herein by examining their procedures and results (targeted proteins and metabolic pathways). These approaches have the potential to provide new insights into our comprehension of antimicrobial resistance/susceptibility, creating new perspectives for the struggle against bacteria, and leading to the development of novel products in the future.

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Pseudomonas fluorescens Pf-5 genome-wide mutant screen for resistance to the antimicrobial peptide alfalfa snakin-1

Cited by 11 publications

References 38 publications

Dynamics of Aspen Roots Colonization by Pseudomonads Reveals Strain-Specific and Mycorrhizal-Specific Patterns of Biofilm Formation

Dynamics of Aspen Roots Colonization by Pseudomonads Reveals Strain-Specific and Mycorrhizal-Specific Patterns of Biofilm Formation

When the Frequencies of Sensitization and Elicitation of Allergic Reaction Do Not Correlate—The Case of Apple Gibberellin-Regulated Protein Tested in an Italian Population

Application of Omics Technologies for Evaluation of Antibacterial Mechanisms of Action of Plant-Derived Products

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