Pseudomonas leptonychotis sp. nov., isolated from Weddell seals in Antarctica

Nováková, Dana; Švec, Pavel; Zeman, Michal; Busse, Hans‐Jürgen; Mašlaňová, Ivana; Pantůček, Roman; Králová, Stanislava; Krištofová, Lucie; Sedláček, Ivo

doi:10.1099/ijsem.0.003753

Cited by 15 publications

(8 citation statements)

References 42 publications

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“…The polyamine pattern consisted of putrescine [67.9 µmol (g dry weight) −1 ], spermidine [15.5 µmol (g dry weight) −1 ], 1,3-diaminopropane [0.6 µmol (g dry weight) −1 ] and traces of cadaverine [>0.1 µmol (g dry weight) −1 ]. This pattern is similar to those detected in several Pseudomonas species [14, 15, 17–19].…”

Section: Full-textsupporting

confidence: 89%

Entomomonas asaccharolytica sp. nov., isolated from Acheta domesticus

Busse¹,

Kämpfer

Szostak³

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

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Strain F2AT, isolated from the cricket Acheta domesticus, was subjected to a polyphasic taxonomic characterization. Cells of the strain were rod-shaped, Gram-stain-negative and catalase- and oxidase-positive. It did not assimilate any carbohydrates. The strain's 16S rRNA gene sequence showed highest similarity to Entomomonas moraniae QZS01T (96.4 %). The next highest similarity values were found to representatives of related genera (<93 %). The genome size of strain F2AT was 3.2 Mbp and the G+C content was 36.4 mol%. Average nucleotide identity values based on blast and MUMmer and average amino acid identity values between strain F2AT and E. moraniae QZS01T were 74.29/74.43, 83.88 and 74.70 %, respectively. The quinone system predominantly contained ubiquinone Q-8. In the polar lipid profile, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified phospholipid were detected. The polyamine pattern consisted of the major compounds putrescine and spermidine. Major fatty acids were C18 : 1 ω7c and C16 : 0 and the hydroxyl acids were C12 : 0 3-OH, C14 : 0 2-OH and C14 : 0 3-OH. The diagnostic diamino acid of the peptidoglycan was meso-diaminopimelic acid. Due to its association with the only species of the genus Entomomonas but its distinctness from E. moraniae we here propose the novel species Entomomonas asaccharolytica sp. nov. F2AT (=CCM 9136T=LMG 32211T).

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Section: Full-textsupporting

confidence: 89%

Entomomonas asaccharolytica sp. nov., isolated from Acheta domesticus

Busse¹,

Kämpfer

Szostak³

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

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“… *The data for the habitat and growth ranges were from original studies [22–25]. In addition, the data for P. peli S1-A32-2 T and P. alcaligenes DSM 50342 T were from Timsy et al .…”

Section: Physiology and Chemotaxonomymentioning

confidence: 99%

Pseudomonas guryensis sp. nov. and Pseudomonas ullengensis sp. nov., isolated from soil

Kim

Jeong

Lee

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

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Two Gram-staining-negative, aerobic, rod-shaped bacteria designated strains SR9T and UL070T, were isolated from soil and subjected to taxonomic characterization. Strain SR9T grew at 10–37 °C (optimum 30 °C), at pH 4.0–10.0 (optimum pH 8.0) and in the presence of 0–1 % NaCl (optimum 0 %), and UL070T at 4–33 °C (optimum 30 °C), at pH 4.0–10.0 (optimum pH 7.0) and in the presence of 0–2 % NaCl (optimum 0 %), respectively. Strain UL070T was motile with flagella. Analysis of 16S rRNA gene sequences indicated that the two strains fell into phylogenetic clusters belonging to the genus Pseudomonas . Both strains SR9T and UL070T were mostly related to Pseudomonas campi S1-A32-2T with 99.70 and 99.01% sequence similarities, and the similarity between the two isolates was 98.90 %. The genome-based in silico analyses indicated that each of the strains SR9T and UL070T was clearly separated from other species of Pseudomonas , as the orthologous average nucleotide identity (OrthoANI) and the digital DNA–DNA hybridization (dDDH) values were no higher than 93.09 and 50.03% respectively with any related species, which were clearly below the cutoff for species distinction. The fatty acid profiles of the two strains mainly consisting of unsaturated components, the presence of ubiquinone 9 (Q-9) as the major respiratory quinone, and phosphatidylethanolamine (PE) and diphosphatidylglycerol (DPG) as the diagnostic polar lipids were consistent with their classification into Pseudomonas . The DNA G+C contents of strains SR9T and UL070T were 63.2 mol% and 63.6 mol% respectively. On the basis of both phenotypic and phylogenetic evidences, each of the isolated strains should be classified as a novel species, for which the names Pseudomonas guryensis sp. nov. (type strain=SR9T=KCTC 82228T=JCM 34509T) and Pseudomonas ullengensis sp. nov. (type strain=UL070T=KCTC 82229T=JCM 34510T) are proposed.

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“…The standard set of physiological and biochemical tests such as shape and color of colonies, morphology of cells and spores, capability of hydrolysis of gelatin, urea, lecithin, starch, Tween 80, esculine, tyrosine and DNA, production catalase, arginine dehydrogenase and acid formation from various carbohydrates, spore formation, growth in presence of 10% and 70% NaCl, growth at various temperatures in range of 30-60 • C or hemolysis were performed at Czech Collection of Microorganisms according to procedures described in [17]. The isolate was also commercially identified using Biolog MicroStation system at Czech Collection of Microorganisms (CCM).…”

Section: Identification and Metabolic And Morphological Characterizatmentioning

confidence: 99%

Introducing the Newly Isolated Bacterium Aneurinibacillus sp. H1 as an Auspicious Thermophilic Producer of Various Polyhydroxyalkanoates (PHA) Copolymers–1. Isolation and Characterization of the Bacterium

et al. 2020

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Extremophilic microorganisms are considered being very promising candidates for biotechnological production of various products including polyhydroxyalkanoates (PHA). The aim of this work was to evaluate the PHA production potential of a novel PHA-producing thermophilic Gram-positive isolate Aneurinibacillus sp. H1. This organism was capable of efficient conversion of glycerol into poly(3-hydroxybutyrate) (P3HB), the homopolyester of 3-hydroxybutyrate (3HB). In flasks experiment, under optimal cultivation temperature of 45 °C, the P3HB content in biomass and P3HB titers reached 55.31% of cell dry mass and 2.03 g/L, respectively. Further, the isolate was capable of biosynthesis of PHA copolymers and terpolymers containing high molar fractions of 3-hydroxyvalerate (3HV) and 4-hydroxybutyrate (4HB). Especially 4HB contents in PHA were very high (up to 91 mol %) when 1,4-butanediol was used as a substrate. Based on these results, it can be stated that Aneurinibacillus sp. H1 is a very promising candidate for production of PHA with tailored material properties.

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Pseudomonas leptonychotis sp. nov., isolated from Weddell seals in Antarctica

Cited by 15 publications

References 42 publications

Entomomonas asaccharolytica sp. nov., isolated from Acheta domesticus

Entomomonas asaccharolytica sp. nov., isolated from Acheta domesticus

Pseudomonas guryensis sp. nov. and Pseudomonas ullengensis sp. nov., isolated from soil

Introducing the Newly Isolated Bacterium Aneurinibacillus sp. H1 as an Auspicious Thermophilic Producer of Various Polyhydroxyalkanoates (PHA) Copolymers–1. Isolation and Characterization of the Bacterium

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