Pseudomonas libanensis sp. nov., a new specie isolated from Lebanese spring waters

Abstract

The taxonomic position of eight fluorescent Pseudomonas isolates, from two Lebanese spring waters, which were previously recognized by numerical analysis as members of a new subcluster (subcluster Vb) was examined. Except for one strain, the new subcluster exhibited internal DNA hybridization values of 76-100%, and 9-53% hybridization was measured with the type or reference strains of other Pseudomonas species. The highest DNA binding value was found with Pseudomonas marginalis strains (37-53%). The G+C content of the DNA of the type strain was 58 mol%. A comparison of 1322 nt of the 16S rRNA gene sequence of the strain representing subcluster Vb (CFML 96-195T) with the sequence of other strains of the genus Pseudomonas revealed that strain CFML 96-195T was part of the 'Pseudomonas fluorescens intrageneric cluster'. On the basis of the results of phenotypic, DNA-DNA and phylogenetic analyses, a new Pseudomonas species, Pseudomonas libanensis sp. nov., is proposed for the seven strains of subcluster Vb. The type strain is P. libanensis CFML 96-195T and has been deposited in the Collection de l'Institut Pasteur (Paris, France) as CIP 105460T. The P. libanensis strains are phenotypically and genotypically homogeneous and can be differentiated from most other fluorescent species by several phenotypic features. Differentiation of P. libanensis and Pseudomonas aeruginosa is based mainly on pyocyanin production; P. libanensis can be differentiated from P. fluorescens (all biovars) by alpha-aminobutyrate assimilation. The clinical significance of P. libanensis is unknown.

Culture free DGGE and cloning based monitoring of changes in bacterial communities of salad due to processing

“…The affiliated enterobacteriaceae had their closest relatives to the genus Kluyvera (98.6%, clone C1) and Klebsiella (99.2%,clone C2). Surprisingly, two sequences were affiliated with members of the family pseudomonadaceae, showing their closest relatives to the species P. libaniensis (98.9%, clone C9) and Pseudomonas grimontii (98.7%, clone C11) and so belong to the fluorescence subgroup (Dabboussi et al, 1999). The appearing band in the packed salad (k) showed a maximum similarity of 98.9% to P. libaniensis (clone C9).…”

Population Structure and Diversity of Phenazine-1-Carboxylic Acid Producing Fluorescent Pseudomonas spp. from Dryland Cereal Fields of Central Washington State (USA)

“…The other very closely related group by 16S rDNA that contained most of the Phz + cluster 1 isolates was significantly different from P. orientalis based upon Biolog data as compared to literature values [40]. Based on Biolog substrate utilization profiles, cluster 3 consists of strains very closely related to P. libanensis, differing from this species only in utilization of malonic acid [41]. Additionally, P. gessardii, another close relative, grows on Dsorbitol, while P. libanensis and members of cluster 3 do not.…”

Pseudomonas mosselii sp. nov., a novel species isolated from clinical specimens.

“…(2 strains). Sixty-seven strains were used as references for numerical taxonomy and DNA-DNA hybridization studies, representing 30 species of the genus Pseudomonas sensu stricto , including 6 newly described Pseudomonas species : Pseudomonas veronii , Pseudomonas rhodesiae , both isolated from French mineral waters, Pseudomonas monteilii (Elomari et al, 1997) isolated from clinical specimens, and ' Pseudomonas cedrella ', ' Pseudomonas orientalis ' (Dabboussi et al, 1999a) and Pseudomonas libanensis (Dabboussi et al, 1999b) isolated from Lebanese spring waters (Table 1). All bacteria were cultured routinely on Mueller-Hinton medium at 30 mC.…”

“…The phenotypic data of the 12 strains of cluster IV (proposed below as P. mosselii sp. nov.) are compared in Table 2 with those of other species, among them the non-phytopathogenic members of section I pseudomonads (Palleroni et al, 1973) : P. aeruginosa, P. fluorescens biovars I-V, P. putida biovar A and B and P. chlororaphis and the newly described species P. veronii , P. rhodesiae , P. monteilii (Elomari et al, 1997), ' P. cedrella ', ' P. orientalis ' (Dabboussi et al, 1999a) and P. libanensis (Dabboussi et al, 1999b).…”

“…monteilii (Elomari et al, 1997), P. libanensis (Dabboussi et al, 1999b), ' P. cedrella ' and P. orientalis (Dabboussi et al, 1999a). The tree constructed by the neighbour-joining method (Saitou & Nei, 1987) and showing phylogenetic relationships of strain CFML 90-83 T with 36 type strains is illustrated in Fig.…”