Pseudomonas lijiangensis sp. nov., a novel phytopathogenic bacterium isolated from black spots of tobacco

Abstract

Three Gram-stain-negative, motile, with amphilophotrichous flagella, and rod-shaped bacteria (LJ1, LJ2T and LJ3) were isolated from lower leaves with black spots on flue-cured tobacco in Yunnan, PR China. The results of phylogenetic analysis based on 16S rRNA gene sequences indicate that all the strains from tobacco were closely related to the type strains of the Pseudomonas syringae group within the P. fluorescens lineage and LJ2T has the highest sequence identities with P. cichorii DSM 50259T (99.92 %), P. capsici Pc19-1T (99.67 %) and P. ovata F51T (98.94 %) . The 16S rRNA gene sequence identities between LJ2T and other members of the genus Pseudomonas were below 98.50%. The average nucleotide identity by blast (ANIb) values between LJ2T and P. cichorii DSM 50259T, P. capsici Pc19-1T and P. ovata F51T were less than 95 %, and the in silico DNA–DNA hybridization (isDDH) values (yielded by formula 2) were less than 70 %. The major fatty acids were C16 : 1ω7c and/or C16 : 1ω6c (summed feature 3), C16 : 0 and C18 : 1ω7c and/or C18 : 1ω6c (summed feature 8). The polar lipids profile of LJ2T consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, two unidentified phospholipids and one unidentified glycolipid. The predominant respiratory quinone was Q-9. The DNA G+C content of LJ2T was 58.4 mol%. On the basis of these data, we concluded that LJ2T represents a novel species of the genus Pseudomonas , for which the name Pseudomonas lijiangensis sp. nov. is proposed. The type strain of Pseudomonas lijiangensis sp. nov. is LJ2T (=CCTCC AB 2021465T=GDMCC 1.2884T=JCM 35177T).

Ralstonia wenshanensis sp. nov., a novel bacterium isolated from a tobacco field in Yunnan, China

“…The protocol used for strains’ physiologic and chemical features analysis was performed as described previously [31]. In brief, growth of strain 56D2 T was determined at 28 °C for 5 days on several agar media, including Luria–Bertani (LB) agar, NA, trypticase soy agar (TSA; BD Difco), casamino acid–peptone–glucose (CPG) agar [32], potato dextrose agar (PDA), modified selective medium South Africa (mSMSA) agar [33] and MacConkey agar (BD Difco).…”

Proposal to classify Ralstonia solanacearum phylotype I strains as Ralstonia nicotianae sp. nov., and a genomic comparison between members of the genus Ralstonia

“…A Gram-staining kit (D008-1-1; Nanjing Jiancheng Bioengineering Institute, Nanjing, China) was used stain the strains, which was detected under a light microscope (Axio imager.Z2; Zeiss, Oberkochen, Germany). The oxygen requirement assay was conducted by streaking the test strain on TSA slope covered with mineral oil and incubating at 28°C for 5 days ( Lu et al, 2022b ).…”