Pseudomonas Lipases: Molecular Genetics and Potential Industrial Applications

Soberón‐Chávez, Gloria; Palmeros, Beatrı́z

doi:10.3109/10408419409113549

Cited by 33 publications

(17 citation statements)

References 52 publications

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“…Accordingly, there are many gene-expression studies on these lipases, mainly including their extracellular secretion from native and recombinant microorganisms (Gupta et al, 2004;Soberon-Chavez and Palmeros, 1994). Cell surface display technology has some interesting advantages in applications such as whole-cell biocatalysis and high-throughput screening (Chen and Georgiou, 2002;Lee et al, 2003;Becker et al, 2007).…”

mentioning

confidence: 99%

Functional display of Pseudomonas and Burkholderia lipases using a translocator domain of EstA autotransporter on the cell surface of Escherichia coli

Yang

Kwon

Song

et al. 2010

Journal of Biotechnology

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confidence: 99%

Functional display of Pseudomonas and Burkholderia lipases using a translocator domain of EstA autotransporter on the cell surface of Escherichia coli

Yang

Kwon

Song

et al. 2010

Journal of Biotechnology

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“…Two different lipases, Burkholderia cepacia (formerly Pseudomonas cepacia) lipase (PS-IM) and Pseudozyma antarctica (formerly Candida antarctica) lipase B (CAL-B), commonly used in organic synthesis, were chosen to test this double dynamic system. [22][23][24] Together with phenyl acetate, one of the activated acyl donors for lipase transesterifications, these two lipases behaved totally different under the same conditions: CAL-B gave no product from either intermediates while PS-IM provided both acetylated products 4A and 4B in different amounts. However, no products from aldehyde 2 or 3 were observed, thus unselected by lipase-catalyzed transesterifications while in the same system with aldehyde 1.…”

mentioning

confidence: 99%

Double parallel dynamic resolution through lipase-catalyzed asymmetric transformation

Zhang

Ramström

2013

Chem. Commun.

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Dynamic systems based on double parallel reactions have been generated and resolved in situ by secondary lipase-catalyzed asymmetric transformation, resulting in high chemo-and enantioselectivities.Over the last few decades, along with the powerful impact of supramolecular chemistry, the pursuit to understand the complexity of life has gained increasing momentum within the scientific community. Living systems noticeably depend on intricate networks of molecular reactions and interactions under both thermodynamic and kinetic control, operating in response to various signals and factors. Some of these features can be addressed using the concept of dynamic chemistry. Derived from the feature of reversibility, dynamic chemistry has been established as a useful methodology for generating and studying complex networks on a molecular scale. At the constitutional level, this has led to molecular networks that enable selection and identification of for example new substrates for targets, receptors for ligands, and functional materials.1-3 The concept is based on the generation of dynamic systems, in which all system components mutually undergo reversible-covalent or non-covalent interactions under thermodynamic control. The adaptive nature of these systems makes them responsive to internal or external pressures, and any changes of the important parameters in the system can rearrange the constituent compositions, leading to the amplification of optimal constituents. Among the variety of selection pressures that have been applied to dynamic systems, secondary, kinetically controlled, enzyme-catalyzed transformations have proven especially efficient in constituent selection, due to their high substrate specificities. 4-10 Lipases, which belong to the hydrolase enzyme family, were most often used in the dynamic systems owing to advantages such as showing high catalytic efficiency and good selectivities, and being environmentally friendly. to their generation and control. Yet, this represents an important development since higher order systems would lead to an increased understanding of molecular complexity in general (Fig. 1). This challenge was addressed in the present study, where new dynamic systems were generated based on two different reversible reactions operating in parallel under the same conditions, providing two types of structures for the secondary enzymatic resolution process. Several requirements need to be met by the reactions operating in such systems: (1) compatibility under the same catalytic conditions; (2) similar kinetic properties with comparable substrate distributions; and (3) no dominant side reactions taking place in the system during the time course. In the present case, the nitroaldol reaction together with hemithioacetal formation were deemed able to fulfill all prerequisites, and further evaluated in a more complex parallel resolution process. The nitroaldol reaction is one of the first C-C bond-forming reactions used for complex dynamic systems, and its reversibility has been confirmed under bas...

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“…Lipases are widely used in pharmaceutical and laundry detergent industries (Izumi et al. 1990;Soberan‐Chavez and Palmeros 1994). They are also used as catalyst for trans‐ and interesterification reaction:…”

Section: Introductionmentioning

confidence: 99%

Role of Saturated Fatty Acids in Lipase Production – Using Pseudomonas Aeruginosa

Saravanan¹,

Suchitra²,

Dhandayuthapani³

2007

J Food Biochemistry

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Complex substrates always induce substantial amount of enzyme production during hydrolysis by microorganisms. In this study, ghee was taken for its saturated fatty acid content and analyzed as an inducer for the production of lipase. With ghee emulsion, the bacterium Pseudomonas aeruginosa at optimal condition produced 60 units/min/L at 72 h. With olive oil emulsion, this organism produced only 41 units/min/L as maximum at 96 h. The saturated fatty acids present in ghee make it a hard substance for hydrolysis, which is the reason for the increased enzyme production. This was evaluated by the iodine number experiment. Ghee can also reduce the production cost whereas the costlier olive oil constitutes 25–50% of the total production cost for a commercial scale. The experimental results showed that the saturated fatty acids play an important role in lipase enzyme induction by P. aeruginosa. The use of ghee is cost‐effective; hence, it can be used as a potential inducer for lipase production. PRACTICAL APPLICATIONS Lipases are industrially very important enzymes. They are used in pharmaceutical, food, soap and other industries. In lipase production, olive oil is the main constituent. Comparatively, olive oil is costlier; hence, it increases the production cost of lipase. So, this study was done to replace olive oil with a much cheaper ghee using Pseudomonas aeruginosa. The ghee‐containing medium gave a very good result because of the presence of complex saturated fatty acids. The ghee‐containing medium produced 60 units/min/L at 72 h. The olive oil medium, which contains mainly unsaturated fatty acids, produced only 41 units/min/L as maximum at 96 h. Hence, in the commercial scale, ghee can reduce raw material cost as well as operation time cost significantly when it is used as substrate.

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Pseudomonas Lipases: Molecular Genetics and Potential Industrial Applications

Cited by 33 publications

References 52 publications

Functional display of Pseudomonas and Burkholderia lipases using a translocator domain of EstA autotransporter on the cell surface of Escherichia coli

Functional display of Pseudomonas and Burkholderia lipases using a translocator domain of EstA autotransporter on the cell surface of Escherichia coli

Double parallel dynamic resolution through lipase-catalyzed asymmetric transformation

Role of Saturated Fatty Acids in Lipase Production – Using Pseudomonas Aeruginosa

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