Pseudomonas matsuisoli sp. nov., isolated from a soil sample

Lin, Shih-Yao; Hameed, Asif; Hung, Mei-Hua; Liu, You‐Cheng; Hsu, Yi-Han; Young, Li-Sen; Young, Chiu-Chung

doi:10.1099/ijs.0.000035

Cited by 12 publications

(6 citation statements)

References 36 publications

(39 reference statements)

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“…Colonies that grew on the plates were picked and purified by repeated streak plating. Isolates that possessed the highest QPE-degrading efficiency were selected and identified based on their morphological, physiological and biochemical properties combined with a 16S rRNA gene sequence analysis as described by Lin et al [12]. …”

Section: Methodsmentioning

confidence: 99%

Purification and properties of a novel quizalofop-p-ethyl-hydrolyzing esterase involved in quizalofop-p-ethyl degradation by Pseudomonas sp. J-2

Zhang

et al. 2017

Microb Cell Fact

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Quizalofop-p-ethyl (QPE) is a post-emergence herbicide that effectively controls grass weeds and is often detected in the environment. However, the biochemical and molecular mechanisms of QPE degradation in the environment remains unclear. In this study, a highly effective QPE-degrading bacterial strain J-2 was isolated from acclimated activated sludge and identified as a Pseudomonas sp., containing the QPE breakdown metabolite quizalofop acid (QA) identified by Liquid Chromatography-Ion Trap-Mass Spectrometry (LC-IT-MSn) analysis. A novel QPE hydrolase esterase-encoding gene qpeH was cloned from strain J-2 and functionally expressed in Escherichia coli BL21 (DE3). The specific activity of recombinant QpeH was 198.9 ± 2.7 U mg−1 for QPE with K m and K cat values of 41.3 ± 3.6 μM and 127.3 ± 4.5 s−1. The optimal pH and temperature for the recombinant QpeH were 8.0 and 30 °C, respectively and the enzyme was activated by Ca2+, Cd2+, Li+, Fe3+ and Co2+ and inhibited by Ni2+, Fe2+, Ag+, DEPC, SDS, Tween 80, Triton X, β-mercaptoethanol, PMSF, and pCMB. In addition, the catalytic efficiency of QpeH toward different AOPP herbicides in descending order was as follows: fenoxaprop-P-ethyl > quizalofop-P-tefuryl > QPE > haloxyfop-P-methyl > cyhalofopbutyl > clodinafop-propargyl. On the basis of the phylogenetic analysis and multiple sequence alignment, the identified enzyme QpeH, was clustered with esterase family V, suggesting a new member of this family because of its low similarity of amino acid sequence with esterases reported previously.

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Section: Methodsmentioning

confidence: 99%

Purification and properties of a novel quizalofop-p-ethyl-hydrolyzing esterase involved in quizalofop-p-ethyl degradation by Pseudomonas sp. J-2

Zhang

et al. 2017

Microb Cell Fact

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“…Hydroxylated fatty acids were C 10 : 0 3-OH and C 12 : 0 3-OH (Table 2). This fatty acid profile was similar to those of species of the genus Pseudomonas but the absence [42]); 3, P. nosocomialis A31/70 T [40]; 4, P. thermotolerans LMG 21284 T ; 5, 'P. sediminis' PI11 [41].…”

Section: Physiology and Chemotaxonomymentioning

confidence: 55%

“…sediminis ’ PI11 [41] the absence of summed feature 2 (C 14 : 0 3-OH and/or iso-C 16:1 I) which distinguished them from P. matsuisoli [42; ]. The significantly lower content of C 18 : 1 ω7 c and/or C 18 : 1 ω6 c distinguished Wesi-4 T from ‘P.…”

Section: Physiology and Chemotaxonomymentioning

confidence: 99%

“…However, the presence of phosphatidylcholine is not a common characteristic of species of the genus Pseudomonas . For instance, the species Pseudomonas azotofigens , Pseudomonas balearica, Pseudomonas knackmussii and Pseudomonas urumqiensis have been reported to lack this compound in their polar lipid profiles [38, 42, 43]. Polyamines were extracted from biomass that had been harvested at the late exponential growth phase as recommended recently [7].…”

Section: Physiology and Chemotaxonomymentioning

confidence: 99%

“…Wesi-4 T shared with P. nosocomialis A31/70 T[40] and 'P. sediminis' PI11[41] the absence of summed feature 2 (C 14 : 0 3-OH and/or iso-C 16:1 I) which distinguished them from P. matsuisoli[42; Table…”

mentioning

confidence: 97%

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Pseudomonas carbonaria sp. nov., isolated from charcoal

Kämpfer

Glaeser

McInroy

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

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A beige-pigmented, oxidase-positive bacterial isolate, Wesi-4T, isolated from charcoal in 2012, was examined in detail by applying a polyphasic taxonomic approach. Cells of the isolates were rod shaped and Gram-stain negative. Examination of the 16S rRNA gene sequence of the isolate revealed highest sequence similarities to the type strains of Pseudomonas matsuisoli and Pseudomonas nosocomialis (both 97.3 %). Phylogenetic analyses on the basis of the 16S rRNA gene sequences indicated a separate position of Wesi-4T, which was confirmed by multilocus sequence analyses (MLSA) based on the three loci gyrB, rpoB and rpoD and a core genome-based phylogenetic tree. Genome sequence based comparison of Wesi-4T and the type strains of P. matsuisoli and P. nosocomialis yielded average nucleotide identity values <95 % and in silico DNA-DNA hybridization values <70 %, respectively. The polyamine pattern contains the major amines putrescine, cadaverine and spermidine. The quinone system contains predominantly ubiquinone Q-9 and in the polar lipid profile diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine are the major lipids. The fatty acid contains predominantly C16 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and summed feature 8 (C18 : 1ω7c and/or C18 : 1 ω6c). In addition, physiological and biochemical tests revealed a clear phenotypic difference from P. matsuisoli . These cumulative data indicate that the isolate represents a novel species of the genus Pseudomonas for which the name Pseudomonas carbonaria sp. nov. is proposed with Wesi-4T (=DSM 110367T=CIP 111764T=CCM 9017T) as the type strain.

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