Pseudomonas-Mediated Mitigation of Salt Stress and Growth Promotion in Glycine max

Kasotia, Amrita; Varma, Ajit; Choudhary, Devendra Kumar

doi:10.1007/s40003-014-0139-1

Cited by 60 publications

(11 citation statements)

References 78 publications

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“…Consequently, several key stress responses as well as mitigation effects were lowered due to diminished osmotic stress, higher chlorophyll levels and cell membrane stability index, as well as lower proline concentrations. Also Kasotia et al 37 reported reduced Na + /K + ratios in soybean plants inoculated with an Pseudomonas koreensis strain and Abdelaziz et al 38 showed similar effects for Arabidopsis seedlings inoculated with P. indica. www.nature.com/scientificreports www.nature.com/scientificreports/…”

Section: Pokkali Endophyte Promotes Growth Of the Salt Sensitive Ricementioning

confidence: 89%

An endophyte from salt-adapted Pokkali rice confers salt-tolerance to a salt-sensitive rice variety and targets a unique pattern of genes in its new host

Sampangi-Ramaiah¹,

Jagadheesh²,

Dey³

et al. 2020

Sci Rep

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Endophytes, both of bacterial and fungal origin, are ubiquitously present in all plants. While their origin and evolution are enigmatic, there is burgeoning literature on their role in promoting growth and stress responses in their hosts. We demonstrate that a salt-tolerant endophyte isolated from salt-adapted Pokkali rice, a Fusarium sp., colonizes the salt-sensitive rice variety IR-64, promotes its growth under salt stress and confers salinity stress tolerance to its host. Physiological parameters, such as assimilation rate and chlorophyll stability index were higher in the colonized plants. Comparative transcriptome analysis revealed 1348 up-regulated and 1078 down-regulated genes in plants colonized by the endophyte. Analysis of the regulated genes by MapMan and interaction network programs showed that they are involved in both abiotic and biotic stress tolerance, and code for proteins involved in signal perception (leucine-rich repeat proteins, receptor-like kinases) and transduction (ca 2+ and calmodulin-binding proteins), transcription factors, secondary metabolism and oxidative stress scavenging. For nine genes, the data were validated by qPCR analysis in both roots and shoots. Taken together, these results show that salt-adapted Pokkali rice varieties are powerful sources for the identification of novel endophytes, which can be used to confer salinity tolerance to agriculturally important, but salt-sensitive rice varieties. Salinity stress is one of the most devastating abiotic stresses that affect growth, development and productivity of major crops. A soil is termed saline if its osmotic pressure is approximately 0.2 M Pa with an electrical conductivity of 4 dS/m or more (equivalent to ~40 mM NaCl) 1,2. It is estimated that at least 50% of the arable land worldwide will be salt affected by the year 2050 and this is expected to further increase due to global climate change 3. Rice (Oryza sativa L.), a major staple food crop, is one of the most salt-sensitive cereals 4. In the Indo-Gangetic Basin in India, an estimated 45% loss in rice production was attributed to salinity stress alone 5,6. The responses of plants to salinity stress are often multi-faceted and complex. In the initial phase, salinity stress manifests itself as osmotic stress with reduction in water uptake by plants. This triggers a range of metabolic and molecular cascades such as inhibition of cell expansion, stomatal conductance, photosynthetic activity, abscisic acid (ABA)-mediated responses followed by stimulation of the SOD and peroxidase activities as well as accumulation of osmolytes like proline. The later phase of salinity stress leads to ionic stress, which is due to the

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Section: Pokkali Endophyte Promotes Growth Of the Salt Sensitive Ricementioning

confidence: 89%

An endophyte from salt-adapted Pokkali rice confers salt-tolerance to a salt-sensitive rice variety and targets a unique pattern of genes in its new host

Sampangi-Ramaiah¹,

Jagadheesh²,

Dey³

et al. 2020

Sci Rep

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“…Antioxidants in plants are considered effective agents that resist oxidative damage under salt stress condition ( Spychalla and Desborough, 1990 ; Mittova et al, 2003 ). It has been reported that inoculation with PGPR can effectively increase the antioxidant activities in plants, such as the levels of CAT, POS, and SOD, which contribute to salt stress tolerance ( Nautiyal et al, 2008 ; Kasotia et al, 2015 ; Ullah and Bano, 2015 ; Islam et al, 2016 ). In this study, significantly higher levels of antioxidant activities in terms of POD, SOD, and CAT were detected in salt-stressed Arabidopsis after exposure to FZB42 VOCs for 20 days compared with the non-exposed plants, but not in those exposed for 15 days ( Figure 4 ).…”

Section: Discussionmentioning

confidence: 99%

Induction of Salt Tolerance in Arabidopsis thaliana by Volatiles From Bacillus amyloliquefaciens FZB42 via the Jasmonic Acid Signaling Pathway

et al. 2020

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Previously, we showed that Bacillus amyloliquefaciens FZB42 can confer salt tolerance in plants by root inoculation under salt stress condition, and the FZB42 volatile organic compounds (VOCs) promoted plant growth and development under non-salt stress condition. In the present study, we investigated the mechanism that allows FZB42 VOCs to confer salt tolerance in Arabidopsis without colonization of plant roots. We found that FZB42 VOCs significantly increased the biomass of Arabidopsis and also maintained the leaf chlorophyll content under salt stress condition. Physiological tests showed that the plant anti-oxidation system was activated by FZB42 VOCs, where higher peroxidase (POD), catalase (CAT), and superoxide dismutase (SOD) activities were detected in plants exposed to FZB42 VOCs compared with non-exposed plants. In addition, FZB42 VOCs increased the leaf total soluble sugars (TSS) content but decreased the proline content compared with the non-exposed plants. Moreover, FZB42 VOCs significantly decreased the Na + contents of the whole plants and induced the expression of genes ( NHX1 ; Na + /H + exchanger 1 and HKT1 ; high-affinity K + transporter 1) that function to alleviate Na + toxicity. Furthermore, analysis of mutants with defects in specific hormone pathways showed that FZB42 VOCs induced salt tolerance in plants by modulating jasmonic acid (JA) signaling, which was confirmed by the up-regulation of JA synthesis, defense-related genes, and JA biosynthesis inhibitor tests. The results of this study provide new insights into the molecular mechanism related to the interactions between plant growth-promoting rhizobacteria and plants under salt stress condition.

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“…Yasmin et al [45] revealed that inoculation of salt-stressed soybean plants with Pseudomonas pseudoalcaligenes triggered synthesis of key defense enzymes that reduced the Na + concentration in roots and shoots simultaneously balancing the cellular condition by increasing intracellular K + levels. Similarly, Pseudomonas koreensis strain AK-1 reduced Na + and elevated K + levels in Glycine max L. Merrill [46] . Treatment of HT-PGPR, Burkholderia phytofirmans PsJN modified the expression patterns of ion-homeostasis related genes including Arabidopsis K + Transporter 1 (AKT1), High-Affinity K + Transporter 1 (HKT1), Sodium Hydrogen Exchanger 2 ( NHX2 ), and SOS1 in salt-sensitive A. thaliana plants [29] .…”

Section: Ht-pgpr Mediated Salt Tolerance In Plantsmentioning

confidence: 96%

Halo-tolerant plant growth promoting rhizobacteria for improving productivity and remediation of saline soils

Arora

Fatima

Mishra

et al. 2020

Journal of Advanced Research

189

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Pseudomonas-Mediated Mitigation of Salt Stress and Growth Promotion in Glycine max

Cited by 60 publications

References 78 publications

An endophyte from salt-adapted Pokkali rice confers salt-tolerance to a salt-sensitive rice variety and targets a unique pattern of genes in its new host

An endophyte from salt-adapted Pokkali rice confers salt-tolerance to a salt-sensitive rice variety and targets a unique pattern of genes in its new host

Induction of Salt Tolerance in Arabidopsis thaliana by Volatiles From Bacillus amyloliquefaciens FZB42 via the Jasmonic Acid Signaling Pathway

Halo-tolerant plant growth promoting rhizobacteria for improving productivity and remediation of saline soils

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