Pseudomonas mediated nutritional and growth promotional activities for sustainable food security

Sah, Stuti; Krishnani, Shweena; Singh, Rajni

doi:10.1016/j.crmicr.2021.100084

Cited by 71 publications

(58 citation statements)

References 75 publications

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“…Pesticides are indispensable in agriculture and play a critical role in fulfilling rising demands for food productivity and quality. − Unfortunately, environmental pollution caused by the widespread use of agrochemicals cannot be ignored. , Biological control has become a more acceptable technique to control plant diseases or pests and overcome environmental challenges. − Pseudomonas species can produce a wide range of novel bioactive metabolites and play an important role in biological control. − …”

Section: Introductionmentioning

confidence: 99%

Production of Antibacterial Questiomycin A in Metabolically Engineered Pseudomonas chlororaphis HT66

Guo

Wang

et al. 2022

J. Agric. Food Chem.

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Pseudomonas chlororaphis has been demonstrated as a valuable source of antimicrobial metabolites for plant disease biocontrol and biopesticide development. Although phenazine-1-carboxylic acid (PCA) secreted by P. chlororaphis has been commercialized as an antifungal biopesticide, it shows poor antibacterial activity. Questiomycin A, with versatile antibacterial activities, is mainly discovered in some well-known phenazine-producing strains but not in Pseudomonas. Its low titer hinders practical applications. In this work, a metabolite was first identified as Questiomycin A in P. chlororaphis-derived strain HT66ΔphzBΔNat. Subsequently, Questiomycin A has been elucidated to share the same biosynthesis process with PCA by gene deletion and in vitro assays. Through rational metabolic engineering, heterologous phenoxazinone synthase introduction, and medium optimization, the titer reached 589.78 mg/L in P. chlororaphis, the highest production reported to date. This work contributes to a better understanding of Questiomycin A biosynthesis and demonstrates a promising approach to developing a new antibacterial biopesticide in Pseudomonas.

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Section: Introductionmentioning

confidence: 99%

Production of Antibacterial Questiomycin A in Metabolically Engineered Pseudomonas chlororaphis HT66

Guo

Wang

et al. 2022

J. Agric. Food Chem.

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“…Pseudomonas are ubiquitous and versatile bacteria that belong to the subclass Gamma of the Proteobacteria [1]. Pseudomonas is a complex genus of rod-shaped, Gram-negative bacteria that demonstrate a wide range of metabolic mechanisms and consequently are present in various niches [2].…”

Section: Introductionmentioning

confidence: 99%

Comparative Genome Analysis of Two Heterotrophic Nitrifying Pseudomonas putida Strains Isolated from Freshwater Shrimp Ponds in Soc Trang Province

et al. 2022

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Nitrogen compounds, especially ammonia, are widely produced in aquaculture systems during cultivation. Ammonia has been investigated as a model compound for use by heterotrophic nitrifying bacteria. Pseudomonas TT321 and Pseudomonas TT322, isolated from shrimp pond water in Soc Trang province, Vietnam, are identified by comparing them with 31 of the closest genomes sequences from the NCBI nucleotide database. The genome sizes of strains TT321 and TT322 were 5,566,241 bp and 5,563,644 bp, respectively. No plasmids were evident in these strains. Genome analysis revealed that TT321 and TT322 belonged to Pseudomonas putida and shared a common ancestor with 33 genomes. Analysis based on the comparison of genomes showed that three genes, carbamate kinase (arcC), glutamine synthetase (Glul), and aminomethyltransferase (amt), are involved in three metabolic pathways. These pathways are: (i) arginine and proline metabolism, (ii) alanine, aspartate and glutamate metabolism, and (iii) glycine, serine and threonine metabolism. These genes may play important roles in ammonia reduction and support bacterial growth via ammonia assimilation.

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“…Cover crops showed a similar level of Pseudomonas abundance, however inorganic fertilizer had higher relative abundance (57.1%) than the poultry litter (55%) treatment (Figure S1b). It has been reported that the genus Pseudomonas promotes phosphate solubilization, nitrogen fixation, induced systemic resistance, and siderophore production, thus favoring plant growth and phytopathogen control, hence acting as a bioinoculant [70][71][72]. The genus, Rhodoplanes depicted higher abundance in cereal rye and native vegetation (4.9%) compared to wheat and vetch (Figure S1a).…”

Section: Impact Of Management Practices On the Bacterial Community Ab...mentioning

confidence: 93%

Differential Response of Soil Microbial Diversity and Community Composition Influenced by Cover Crops and Fertilizer Treatments in a Dryland Soybean Production System

Narayana

Kingery

Shankle³

et al. 2022

Agronomy

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The response of soil microbial communities to management practices is composite, as it depends on the various environmental factors which contribute to a shift in the microbial communities. In this study we explored the impact of combinations of soil management practices on microbial diversity and community composition in a dryland soybean production system. Soil samples were collected from the experimental field maintained under no till, cover crops, and fertility treatments, at Pontotoc Ridge-Flatwoods Branch Experiment Station, MS, USA. Targeted amplicon sequencing of 16S rRNA and ITS2 genes was used to study the bacterial and fungal community composition. Poultry litter amendment and cover crops significantly influenced soil bacterial diversity. Fertilizer sources had significantly different bacterial communities, as specific microbial taxa were strongly influenced by the changes in the nutrient availability, while cover crops influenced the soil fungal community differences. Differential enrichment of advantageous bacterial (Proteobacteria, Actinobacteria and Acidobacteria) and fungal (Mortierellomycota) phyla was observed across the treatments. Soil pH and easily extractable glomalin-related soil proteins (EE-GRSP) were correlated with bacterial communities and aggregate stability (WSA) was influenced by the poultry litter amendment, thus driving the differences in bacterial and fungal communities. These findings suggest that a long-term study would provide more inferences on soil microbial community response to management changes in these dryland soybean production systems.

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Pseudomonas mediated nutritional and growth promotional activities for sustainable food security

Cited by 71 publications

References 75 publications

Production of Antibacterial Questiomycin A in Metabolically Engineered Pseudomonas chlororaphis HT66

Production of Antibacterial Questiomycin A in Metabolically Engineered Pseudomonas chlororaphis HT66

Comparative Genome Analysis of Two Heterotrophic Nitrifying Pseudomonas putida Strains Isolated from Freshwater Shrimp Ponds in Soc Trang Province

Differential Response of Soil Microbial Diversity and Community Composition Influenced by Cover Crops and Fertilizer Treatments in a Dryland Soybean Production System

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