Pseudomonas nicosulfuronedens sp. nov., a nicosulfuron degrading bacterium, isolated from a microbial consortium

Abstract

A Gram-stain-negative, aerobic, motile, short-rod-shaped bacterium with nicosulfuron-degrading ability, designated strain LAM1902T, was isolated from a microbial consortium enriched with nicosulfuron as a sole nitrogen and energy source. The optimal temperature and pH for growth of strain LAM1902T were 30 °C and pH 6.0, respectively. Strain LAM1902T could grow in the presence of NaCl with concentration up to 4.0 % (w/v). Comparative analysis of 16S rRNA gene sequences revealed that LAM1902T was closely related to the members of the family Pseudomonadaceae to the genus Pseudomonas , with the highest similarity to Pseudomonas nitroreducens DSM 14399T (99.6 %), Pseudomonas nitritireducens WZBFD3-5A2T (99.3 %) and Pseudomonas panipatensis Esp-1T (98.8 %). Multi-locus sequence analysis based on both concatenated sequences of the 16S rRNA gene and three housekeeping genes (gyrB, rpoB and rpoD) further confirmed the intrageneric phylogenetic position of strain LAM1902T. The genomic DNA G+C content of LAM1902T was 64.8 mol%. The low values of in silico DNA–DNA hybridization (less than 43.7 %) and average nucleotide identity (less than 90.9 %) also showed that the strain was distinctly different from known species of the genus Pseudomonas . The major fatty acids were C16 : 0, C17 : 0 cyclo and anteiso C15 : 0. Ubiquinone Q-9 was detected as the predorminant respiratory quinone. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and aminophospholipid. Based on phylogenetic, phenotypic and chemotaxonomic analyses and genome comparisons, we conclude that strain LAM1902T represents a novel species, for which the name Pseudomonas nicosulfuronedens sp. nov. is proposed. The type strain is LAM1902T (=JCM 33860T=KCTC 72830T).

Comamonas resistens sp. nov. and Pseudomonas triclosanedens sp. nov., two members of the phylum Pseudomonadota isolated from the wastewater treatment system of a pharmaceutical factory

“… P. nicosulfuronedens LAM1902 T was provided by the Chinese Academy of Agricultural Sciences (Beijing, PR China) [62]. These strains were routinely cultivated on LB medium at 30 °C.…”

“…testosteroni ATCC 11996 T and C. thiooxydans DSM 17888 T were purchased from Guangdong Microbial Culture Collection Center (Guangzhou, Guangdong province, PR China). P. nicosulfuronedens LAM1902 T was provided by the Chinese Academy of Agricultural Sciences (Beijing, PR China) [62]. These strains were routinely cultivated on LB medium at 30 °C.…”

Molecular Response Mechanism of Pseudomonas Nicosulfuronedens LAM1902 to A Typical Sulfonylurea Herbicide Nicosulfuron

“…In the present study, strain P. nicosulfuronedens LAM1902 can degrade multiple sulfonylurea herbicides (NS, chlorimuronethyl, and cinosulfuron, 50 mg/L) in the incubation medium. In addition, the strain LAM1902 can tolerate and degrade NS under 500 mg NS/L exposure 33,24 . This strain could be used as a mechanistic tool to extrapolate results from laboratory bioassays (liquid condition) to the eld (soil condition) to better understand the bioremediation of sulfonylurea herbicide residues in the real environment.…”

“…Microbial degradation is considered as an environmentally friendly technology to mitigate hazardous residual concentrations of sulfonylurea herbicides in the environment [22][23][24] . There are many bacteria and fungi harboring NS degradation ability, including Alcaligenes faecalis ZWS11 25 , Bacillus subtilis YB1 26 , Klebsiella sp.…”