Pseudomonas putida and Bacillus amyloliquefaciens alleviates the adverse effect of pesticides and poise soil enzymes activities in chickpea (Cicer arietinum L.) rhizosphere

Abstract

Abstract:Pesticide application for disease management is a major action for crop protection from last seven decades. The repeated application of pesticide is the most important cause of the reduction in microbial population. Soil microorganisms play an important role in efficient acquisition and transportation of nutrients to plant. Pesticides leached in soil disturb the activities of soil enzymes, such as β-glucosidase, dehydrogenase, phosphatases, protease and urease secreted by these microorganisms. This drastically reduces nutrient availability to the plants and soil fertility. In vitro experimental studies revealed that our PGPR (Pseudomonas putida and Bacillus amyloliquefaciens) have the ability to tolerate pesticides at concentrations such as Carbendazim (0.512%), Imidacloprid (3.27%) and Glyphosate (3.27%). We have observed an increase in PGP activities like IAA production, exopolysachchride production, biofilm synthesis, phosphate solubilization and siderophore production on the addition of pesticides at concentrations below there threshold values, on the contrary reduction in activities was noticed above these values. Soil enzymes activities from chickpea rhizosphere without PGPR inoculation showed variability on the application of pesticides whereas activities were found normal or increased with PGPR inoculation and pesticides application. Thus PGPR remains panacea for soils by managing adverse effects of pesticide application. Hence our results concluded that P. putida and B. amyloliquefaciens have the ability to reduce the negative impact of three pesticides and poise soil enzymes activities. Hence our PGPR acts as efficient biofertilizers to improve soil fertility and soil health.

Plant-Growth-Promoting Rhizobacteria Emerging as an Effective Bioinoculant to Improve the Growth, Production, and Stress Tolerance of Vegetable Crops

“…Thus, PGPR are emerging as organic fertilizers suitable for many plant species, which could reduce chemical fertilizer application while enhancing soil quality and plant yield [49]. The PGPR species Pseudomonas putida and Bacillus amyloliquefaciens decreased the negative impact of three pesticides (carbendazim, imidacloprid, and glyphosate), maintained soil enzyme activities, and enhanced soil health and fertility [50].…”

Tolerance of pesticides and antibiotics among beneficial soil microbes recovered from contaminated rhizosphere of edible crops

“…As a result, siderophore (an iron chelating compound) are synthesized under Fe starved condition by microbial communities. This is useful because strains that produce siderophore might be employed in phytopathogens bio management ( Kumar et al., 2017 ). Siderophore ( e.g.…”

Mesorhizobium ciceri as biological tool for improving physiological, biochemical and antioxidant state of Cicer aritienum (L.) under fungicide stress

“…Kumar et al . 77 reported that pesticide-tolerant PGPR strains P. putida and B. amyloliquefaciens alleviated the adverse effect of pesticides and increased soil enzyme activities and seed germination efficiency, elongated plant organs and enhanced other parameters of C. arietinum . These findings corroborate our facts that inoculated PGPR degrade/detoxify pesticides and thus, improve various parameters of legumes grown in the affected soil.…”