Pseudomonas putida and its close relatives: mixing and mastering the perfect tune for plants

Abstract

Plant growth–promoting rhizobacteria (PGPR) are a group of microorganisms of utmost interest in agricultural biotechnology for their stimulatory and protective effects on plants. Among the various PGPR species, some Pseudomonas putida strains combine outstanding traits such as phytohormone synthesis, nutrient solubilization, adaptation to different stress conditions, and excellent root colonization ability. In this review, we summarize the state of the art and the most relevant findings related to P. putida and its close relatives as PGPR, and we have compiled a detailed list of P. putida sensu stricto, sensu lato, and close relative strains that have been studied for their plant growth–promoting characteristics. However, the mere in vitro analysis of these characteristics does not guarantee correct plant performance under in vivo or field conditions. Therefore, the importance of studying adhesion and survival in the rhizosphere, as well as responses to environmental factors, is emphasized. Although numerous strains of this species have shown good performance in field trials, their use in commercial products is still very limited. Thus, we also analyze the opportunities and challenges related to the formulation and application of bioproducts based on these bacteria. Key points •The mini-review updates the knowledge on Pseudomonas putida as a PGPR. • Some rhizosphere strains are able to improve plant growth under stress conditions. • The metabolic versatility of this species encourages the development of a bioproduct.

Harnessing the genomic diversity of Pseudomonas strains against lettuce bacterial pathogens

“…fluorescens group ( Figure 4 ). The latter group has been extensively studied for its numerous PGPR isolates, contrary to the former ( Höfte, 2021 ; Costa-Gutierrez et al, 2022 ). Among the 15 isolated strains belonging to the P .…”

“…protegens , and P . putida ( Paulin et al, 2017 ; Nelkner et al, 2019 ; Höfte, 2021 ; Costa-Gutierrez et al, 2022 ). This diversity arose thanks to the numerous soil sample collection sites and the discriminating strain isolation process, based only on a Pseudomonas -selective medium and a binary inhibition criterion against the three lettuce bacterial pathogens.…”

Update of the list of qualified presumption of safety (QPS) recommended microbiological agents intentionally added to food or feed as notified to EFSA 19: Suitability of taxonomic units notified to EFSA until September 2023

“…Body of knowledge P. putida is found mostly in temperate soil and aquatic habitats. Strains of P. putida have plant growth promoting abilities (Costa-Gutierrez et al, 2022) and biofilm forming capacities (Espinosa-Urgel & Ramos-González, 2023). The metabolic versatility of P. putida makes this organism attractive for biotechnological applications and it has been developed as a useful microbial chassis for synthetic biology applications (Martin-Pascual et al, 2021).…”

An iron fist in a velvet glove: The cooperation of a novel pyoverdine from Pseudomonas donghuensisP482 with 7‐hydroxytropolone is pivotal for its antibacterial activity

“…Fluorescent pseudomonads exploit the efficient iron uptake and transport system, which, combined with their metabolic versatility, makes them active players in plant‐related environments (Cornelis, 2010; Gu et al, 2020; Kramer et al, 2020; Raaijmakers et al, 2009). Pseudomonads are readily isolated from the rhizosphere and possess properties beneficial for the host plants (Calderon et al, 2015; Costa‐Gutierrez et al, 2022; Krzyzanowska et al, 2012; Mazurier et al, 2009; Ramette et al, 2011; Zboralski et al, 2020).…”