Pseudomonas putida WH-B3 degrades benzoic acid and alleviates its autotoxicity to peach (Prunus persica L. batsch) seedlings grown in replanted soil

He, Hao; Zhu, Wei; Noor, Iqra; Liu, Junwei; Li, Guohuai

doi:10.1016/j.scienta.2019.05.020

Cited by 15 publications

(12 citation statements)

References 57 publications

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“…Allelochemicals, metabolites produced by living organisms that have a detrimental effect on other species when released into the environment, significantly inhibit plant growth [11,37]. The use of microorganisms to degrade allelochemicals such as benzoic acid and for soil remediation has proven to be effective [38,39]. In this study, using an enrichment and domestication strategy, a benzoic acid-degrading bacterium, SCB32, was isolated and revealed to be a member of the genus Pseudomonas.…”

Section: Discussionmentioning

confidence: 94%

“…putida ATCC 49451, substrate inhibition was observed at benzoate concentrations higher than 500 mg L −1 [ 21 ]. Strain WH-B3 degrades more than 90% of benzoate at a concentration of 500 mg L −1 within 12 h [ 38 ]. Compared with them, stain SCB32 has a higher degradation capacity.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, autotoxicity of the final degradation products of pollutants is of interest. Lettuce ( Lactuca sativa L.) is generally accepted as an allelochemical-sensitive plant species, and benzoic acid has a significant toxic effect on it [ 38 , 48 ]. In this work, benzoic acid (800 mg L −1 ) was degraded by strain SCB32 within 48 h, and the residues of degradation products were phenotypically nontoxic to lettuce seed germination.…”

Section: Discussionmentioning

confidence: 99%

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Complete Genome Sequence and Biodegradation Characteristics of Benzoic Acid‐Degrading Bacterium Pseudomonas sp. SCB32

Xiang

Wei

Tang

et al. 2020

BioMed Research International

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Allelochemicals are metabolites produced by living organisms that have a detrimental effect on other species when released into the environment. These chemicals play critical roles in the problems associated with crop replanting. Benzoic acid is a representative allelochemical found in root exudates and rhizosphere soil of crops and inhibits crop growth. The bioremediation of allelochemicals by microorganisms is an efficient decontamination process. In this research, a bacterial strain capable of degrading benzoic acid as the sole carbon source was isolated. The genome of the strain was sequenced, and biodegradation characteristics and metabolic mechanisms were examined. Strain SCB32 was identified as Pseudomonas sp. based on 16S rRNA gene analysis coupled with physiological and biochemical analyses. The degradation rate of 800 mg L-1 benzoic acid by strain SCB32 was greater than 97.0% in 24 h. The complete genome of strain SCB32 was 6.3 Mbp with a GC content of 64.6% and 5960 coding genes. Potential benzoic acid degradation genes were found by comparison to the KEGG database. Some key intermediate metabolites of benzoic acid, such as catechol, were detected by gas chromatography-mass spectrometry. The biodegradation pathway of benzoic acid, the ortho pathway, is proposed for strain SCB32 based on combined data from genome annotation and mass spectrometry. Moreover, the benzoic acid degradation products from strain SCB32 were essentially nontoxic to lettuce seedlings, while seeds in the benzoic acid-treated group showed significant inhibition of germination. This indicates a possible application of strain SCB32 in the bioremediation of benzoic acid contamination in agricultural environments.

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Complete Genome Sequence and Biodegradation Characteristics of Benzoic Acid‐Degrading Bacterium Pseudomonas sp. SCB32

Xiang

Wei

Tang

et al. 2020

BioMed Research International

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“…Peach ( Prunus persica ) is an economically important fruit crop worldwide, with a global yield of approximately 25 million tons annually, of which above half are produced in China ( Zheng et al, 2021 ). With the increased demand and the reduced cultivated land acreage of peach, replant problem (also known as replant disease) has become increasingly prominent ( He et al, 2019 ). Replant problem refers to a phenomenon of the successive plantation of same or similar plant species on the same land, resulting in reductions of crop yield and quality under usual cultivation practices ( Zhu et al, 2017 ; Yin et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

“…Replant problem refers to a phenomenon of the successive plantation of same or similar plant species on the same land, resulting in reductions of crop yield and quality under usual cultivation practices ( Zhu et al, 2017 ; Yin et al, 2018 ). Of the stone fruits, peach replant problem is much more serious than other species within the Prunus genus owing to the shorter life cycle of peach trees and fast replacement of new cultivars, and has become an important factor hindering the sustainable development of peach industry ( He et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Integrative Physiological, Transcriptional, and Metabolic Analyses Provide Insights Into Response Mechanisms of Prunus persica to Autotoxicity Stress

et al. 2021

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Autotoxicity is known as a critical factor in replanting problem that reduces land utilization and creates economic losses. Benzoic acid (BA) is identified as a major autotoxin in peach replant problem, and causes stunted seedling growth or even death. However, the physiological and molecular mechanisms of peach response to BA stress remain elusive. Here, we comprehensively studied the morphophysiological, transcriptional, and metabolic responses of peach plants to BA toxicity. Results showed that BA stress inhibited peach seedlings growth, decreased chlorophyll contents and fluorescence levels, as well as disturbed mineral metabolism. The contents of hydrogen peroxide, superoxide anion, and malondialdehyde, as well as the total antioxidant capacity, were significantly increased under BA stress. A total of 6,319 differentially expressed genes (DEGs) were identified after BA stress, of which the DEGs related to photosynthesis, redox, and ion metabolism were greatly changed; meanwhile, numerous stress-responsive genes (HSPs, GSTs, GR, and ABC transporters) and transcription factors (MYB, AP2/ERF, NAC, bHLH, and WRKY) were noticeably altered under BA stress. BA induced metabolic reprogramming, and 74 differentially accumulated metabolites, including amino acids and derivatives, fatty acids, organic acids, sugars, and sugar alcohols, were identified in BA-stressed roots. Furthermore, an integrated analysis of genes and metabolites indicated that most of the co-mapped KEGG pathways were enriched in amino acid and carbohydrate metabolism, which implied a disturbed carbon and nitrogen metabolism after BA stress. The findings would be insightful in elucidating the mechanisms of plant response to autotoxicity stress, and help guide crops in alleviating replant problem.

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Mitigation of atrazine-induced oxidative stress on soybean seedlings after co-inoculation with atrazine-degrading bacterium Arthrobacter sp. DNS10 and inorganic phosphorus-solubilizing bacterium Enterobacter sp. P1

Jiang

Shao²,

Chu³

et al. 2022

Environ Sci Pollut Res

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Pseudomonas putida WH-B3 degrades benzoic acid and alleviates its autotoxicity to peach (Prunus persica L. batsch) seedlings grown in replanted soil

Cited by 15 publications

References 57 publications

Complete Genome Sequence and Biodegradation Characteristics of Benzoic Acid‐Degrading Bacterium Pseudomonas sp. SCB32

Complete Genome Sequence and Biodegradation Characteristics of Benzoic Acid‐Degrading Bacterium Pseudomonas sp. SCB32

Integrative Physiological, Transcriptional, and Metabolic Analyses Provide Insights Into Response Mechanisms of Prunus persica to Autotoxicity Stress

Mitigation of atrazine-induced oxidative stress on soybean seedlings after co-inoculation with atrazine-degrading bacterium Arthrobacter sp. DNS10 and inorganic phosphorus-solubilizing bacterium Enterobacter sp. P1

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