Pseudomonas qingdaonensis sp. nov., an aflatoxin-degrading bacterium, isolated from peanut rhizospheric soil

Wang, Mingqing; Wang, Zhen; Yu, Li; Zhang, Chushu; Bi, Jie; Sun, Jiazhi

doi:10.1007/s00203-019-01636-w

Cited by 23 publications

(23 citation statements)

References 28 publications

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“…The major fatty acids (>5 % of the total fatty acids) of MAFF 212427 T and MAFF 212428 were summed feature 3 (29.0, 29.5 %), C 16 : 0 (Table 3). This fatty acid profile was qualitatively similar to those of the type strains of the most closely related species, although quantitative differences in the contents of several fatty acids were detected between them (Table 3) [11,13].…”

Section: Chemotaxonomic Analysessupporting

confidence: 61%

“…Based on the results, they are now categorized into three lineages (Pseudomonas fluorescens, Pseudomonas aeruginosa and Pseudomonas pertucinogena lineages) and these lineages are further subdivided into groups and subgroups [3][4][5][6]. Of these, the Pseudomonas putida group, belonging to the P. fluorescens lineage, has been found to contain 24 species so far (Table S1, available in the online version of this article) [3][4][5][6][7][8][9][10][11][12][13][14][15]. These include Pseudomonas fulva, Pseudomonas parafulva and Pseudomonas cremoricolorata isolated from rice paddies, and Pseudomonas guariconensis and 'Pseudomonas qingdaonensis' derived from the rhizospheric soil of legumes.…”

mentioning

confidence: 99%

“…Data for 'P. qingdaonensis' JJ3 T were obtained from Wang et al[11] and for P. huaxiensis WCHPs060044 T from Qin et al[13] under the same conditions as this study. tr, Trace amount (< 1%); -, not detected.…”

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confidence: 99%

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Pseudomonas brassicae sp. nov., a pathogen causing head rot of broccoli in Japan

Sawada

Fujikawa

Horita

2020

International Journal of Systematic and Evolutionary Microbiology

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Phytopathogenic bacteria, MAFF 212426, MAFF 212427T, MAFF 212428 and MAFF 212429, were isolated from head rot lesions of broccoli (Brassica oleracea L. var. italica Plenck) in Hokkaido, Japan, and subjected to polyphasic taxonomic characterization. The cells were Gram-reaction-negative, aerobic, non-spore-forming, motile with one or two polar flagella, rod-shaped and formed pale yellow colonies. Results of 16S rRNA gene sequence analysis showed that they belong to the genus Pseudomonas with the highest similarity to ‘Pseudomonas qingdaonensis’ JJ3T (99.86 %), Pseudomonas laurentiana GSL-010T (99.22 %), Pseudomonas huaxiensis WCHPs060044T (99.01 %), Pseudomonas japonica NBRC 103040T (98.87 %) and Pseudomonas alkylphenolica KL28T (98.73 %). The genomic DNA G+C content was 63.4 mol% and the major fatty acids (>5 % of the total fatty acids) were summed feature 3 (C16 : 1 ω7c / C16 : 1 ω6c), C16 : 0, summed feature 8 (C18 : 1 ω7c / C18 : 1 ω6c) and C17 : 0 cyclo. Multilocus sequence analysis using the partial rpoD, gyrB and rpoB gene sequences and phylogenomic analyses based on the whole genome sequences demonstrated that the strains are members of the Pseudomonas putida group, but form a monophyletic, robust clade separated from their closest relatives. Average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values corroborated their novel species status, with 88.39 % (ANI) and 35.8 % (dDDH) as the highest scores with ‘P. qingdaonensis’ JJ3T. The strains were differentiated from their closest relatives by phenotypic characteristics, pathogenicity on broccoli, and whole-cell MALDI-TOF mass spectrometry profiles. The phenotypic, chemotaxonomic and genotypic data showed that the strains represent a novel Pseudomonas species, for which the name Pseudomonas brassicae sp. nov. is proposed. The type strain is MAFF 212427T (=ICMP 23635T).

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Section: Chemotaxonomic Analysessupporting

confidence: 61%

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confidence: 99%

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Pseudomonas brassicae sp. nov., a pathogen causing head rot of broccoli in Japan

Sawada

Fujikawa

Horita

2020

International Journal of Systematic and Evolutionary Microbiology

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“…On the basis of their 16S rDNA sequences, the strains harbouring the orthologous cluster are representatives of P. donghuensis (HYS, P482, Irchel 3F5, 1239, ABAC8, NBRC 111117 and RIT263), P. qingdaonensis (JJ3, BIGb0473, PA14H7, 1033, MF6396, BRM28 and UASWS0946) and P. wadenswilerensis (SNU WT1), being the three of them recently described species within the broad P. putida group (Gao et al, 2015;Frasson et al, 2017;Wang et al, 2019) (Fig. S1).…”

Section: Identification Of a Gene Cluster Involved In Fungal Antagonismmentioning

confidence: 99%

7‐hydroxytropolone is the main metabolite responsible for the fungal antagonism of Pseudomonas donghuensis strain SVBP6

Muzio

Agaras

Masi

et al. 2020

Environmental Microbiology

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Summary Pseudomonas donghuensis strain SVBP6, an isolate from an agricultural plot in Argentina, displays a broad‐spectrum and diffusible antifungal activity, which requires a functional gacS gene but could not be ascribed yet to known secondary metabolites typical of Pseudomonas biocontrol species. Here, we report that Tn5 mutagenesis allowed the identification of a gene cluster involved in both the fungal antagonism and the production of a soluble tropolonoid compound. The ethyl acetate extract from culture supernatant showed a dose‐dependent inhibitory effect against the phytopathogenic fungus Macrophomina phaseolina. The main compound present in the organic extract was identified by spectroscopic and X‐ray analyses as 7‐hydroxytropolone (7HT). Its structure and tautomerism was confirmed by preparing the two key derivatives 2,3‐dimethoxy‐ and 2,7‐dimethoxy‐tropone. 7HT, but not 2,3‐ or 2,7‐dimethoxy‐tropone, mimicked the fungal inhibitory activity of the ethyl acetate extract from culture supernatant. The activity of 7HT, as well as its production, was barely affected by the presence of up to 50 μM added iron (Fe+2). To summarize, P. donghuensis SVBP6 produces 7HT under the positive control of the Gac‐Rsm cascade and is the main active metabolite responsible for the broad‐spectrum inhibition of different phytopathogenic fungi.

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“…As shown in Tables 2 and 3 The 16S rRNA gene sequences and housekeeping genes (gyrB, rpoB and rpoD) of P. asiatica, P. asplenii, P. fuscovaginae and P. shirazica were obtained from the NCBI GenBank database (accession numbers in Table S1, available in the online version of this article) and were compared with each other. Between P. asplenii and *Species were published (P. alloputida [17], P. batumici [35], P. hutmensis [16], P. persica [17] and P. qingdaonensis [21]) but are not listed in a Validation List. The literature was reviewed to assess whether these strains have been reported to possess distinctive characteristics of phenotypes.…”

mentioning

confidence: 99%

Genome analysis-based reclassification of Pseudomonas fuscovaginae and Pseudomonas shirazica as later heterotypic synonyms of Pseudomonas asplenii and Pseudomonas asiatica, respectively

Tohya

Watanabe

Tada

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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This study was conducted to clarify the taxonomic status of the species Pseudomonas fuscovaginae and Pseudomonas shirazica . Whole genome sequences for the type strains of P. fuscovaginae and P. shirazica were compared against the closely related type strains of the Pseudomonas putida group and the Pseudomonas fluorescens group species. Average nucleotide identity and digital DNA–DNA hybridization values between P. fuscovaginae LMG 2158T and Pseudomonas asplenii ATCC 23835T were 98.4 and 85.5 %, and between P. shirazica VM14T and Pseudomonas asiatica RYU5T were 99.3 and 95.3 %. These values were greater than recognized thresholds for bacterial species delineation, indicating that they belong to the same genomospecies, respectively. Therefore, P. fuscovaginae and P. shirazica should be reclassified as later heterotypic synonyms of P. asplenii and P. asiatica , respectively.

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Pseudomonas qingdaonensis sp. nov., an aflatoxin-degrading bacterium, isolated from peanut rhizospheric soil

Cited by 23 publications

References 28 publications

Pseudomonas brassicae sp. nov., a pathogen causing head rot of broccoli in Japan

Pseudomonas brassicae sp. nov., a pathogen causing head rot of broccoli in Japan

7‐hydroxytropolone is the main metabolite responsible for the fungal antagonism of Pseudomonas donghuensis strain SVBP6

Genome analysis-based reclassification of Pseudomonas fuscovaginae and Pseudomonas shirazica as later heterotypic synonyms of Pseudomonas asplenii and Pseudomonas asiatica, respectively

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