Pseudomonas soli sp. nov., a novel producer of xantholysin congeners

Pascual, Javier; García-López, Marina; Reyes-Carmona, Cristina; Sousa, Thiciana da Silva; Pedro, Nuria de; Cautain, Bastien; Martín, Jesús; Vicente, Francisca; Reyes, Fernando; Bills, Gerald F.; Genilloud, Olga

doi:10.1016/j.syapm.2014.07.003

Cited by 38 publications

(29 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thirteen CLP types were characterized during this study namely xantholysin, entolysin, WLIP, lokisin, putisolvin and eight novel ones designated cocoyamide A (N1), N2, N3, N4, N5, N6, N7 and N8. Xantholysin production was previously reported in a banana rhizosphere isolate, P. putida BW11M1 (Li et al ., ) and in P. soli , isolated from a soil sample collected from Spain (Pascual et al ., ). So far, entolysin biosynthesis has only been reported for one strain, P. entomophilia L48 T (Vallet‐Gely et al ., ).…”

Section: Discussionmentioning

confidence: 97%

Fluorescent Pseudomonas and cyclic lipopeptide diversity in the rhizosphere of cocoyam (Xanthosoma sagittifolium)

Oni

Geudens

Omoboye

et al. 2019

Environmental Microbiology

126

View full text Add to dashboard Cite

Summary Cocoyam (Xanthosoma sagittifolium (L.)), an important tuber crop in the tropics, is severely affected by the cocoyam root rot disease (CRRD) caused by Pythium myriotylum. The white cocoyam genotype is very susceptible while the red cocoyam has some field tolerance to CRRD. Fluorescent Pseudomonas isolates obtained from the rhizosphere of healthy red and white cocoyams from three different fields in Cameroon were taxonomically characterized. The cocoyam rhizosphere was enriched with P. fluorescens complex and P. putida isolates independent of the plant genotype. LC–MS and NMR analyses revealed that 50% of the Pseudomonas isolates produced cyclic lipopeptides (CLPs) including entolysin, lokisin, WLIP, putisolvin and xantholysin together with eight novel CLPs. In general, CLP types were linked to specific taxonomic groups within the fluorescent pseudomonads. Representative CLP‐producing bacteria showed effective control against CRRD while purified CLPs caused hyphal branching or hyphal leakage in P. myriotylum. The structure of cocoyamide A, a CLP which is predominantly produced by P. koreensis group isolates within the P. fluorescens complex is described. Compared with the white cocoyam, the red cocoyam rhizosphere appeared to support a more diverse CLP spectrum. It remains to be investigated whether this contributes to the field tolerance displayed by the red cocoyam.

show abstract

Section: Discussionmentioning

confidence: 97%

Fluorescent Pseudomonas and cyclic lipopeptide diversity in the rhizosphere of cocoyam (Xanthosoma sagittifolium)

Oni

Geudens

Omoboye

et al. 2019

Environmental Microbiology

126

View full text Add to dashboard Cite

show abstract

“…Focusing on the latter, Pseudomonas CLiPs (Ps-CLiPs) are involved in cell motility, adhesion and biofilm formation (Alsohim et al, 2014;D'aes et al, 2014;Song et al, 2014), but also have functions in bacterial ecology as they promote plant-growth (D'aes et al, 2010;Alsohim et al, 2014) or can trigger defense responses in plants (Nielsen et al, 1999;D'aes et al, 2014). In addition to antibiotic activity (Sinnaeve et al, 2009b;Jang et al, 2013), antagonistic properties including antifungal (Gerard et al, 1997), insecticidal (Groupe et al, 1951), antiviral (Van De Mortel et al, 2009), and anti-oomycete activity have been established (Saini et al, 2008) while more recently antitumor activity has also been reported (Pascual et al, 2014;Cautain et al, 2015;Götze and Stallforth, 2019). Thus, Ps-CLiPs appear as veritable biological 'swiss-army knives' , a trait which is increasingly recognized for AMPs in general (Haney et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Identification of the Molecular Determinants Involved in Antimicrobial Activity of Pseudodesmin A, a Cyclic Lipopeptide From the Viscosin Group

et al. 2020

View full text Add to dashboard Cite

Cyclic lipo(depsi)peptides (CLiPs) from Pseudomonas constitute a class of natural products involved in a broad range of biological functions for their producers. They also display interesting antimicrobial potential including activity against Gram-positive bacteria. Literature has indicated that these compounds can induce membrane permeabilization, possibly through pore-formation, leading to the general view that the cellular membrane constitutes the primary target in their mode of action. In support of this view, we previously demonstrated that the enantiomer of pseudodesmin A, a member of the viscosin group of CLiPs, shows identical activity against a test panel of six Gram-positive bacterial strains. Here, a previously developed total organic synthesis route is used and partly adapted to generate 20 novel pseudodesmin A analogs in an effort to derive links between molecular constitution, structure and activity. From these, the importance of a macrocycle closed by an ester bond as well as a critical length of β-OH fatty acid chain capping the N-terminus is conclusively demonstrated, providing further evidence for the importance of peptidemembrane interactions in the mode of action. Moreover, an alanine scan is used to unearth the contribution of specific amino acid residues to biological activity. Subsequent interpretation in terms of a structural model describing the location and orientation of pseudodesmin A in a membrane environment, allows first insight in the peptide-membrane interactions involved. The biological screening also identified residue positions that appear less sensitive to conservative modifications, allowing the introduction of a non-perturbing tryptophan residue which will pave the way toward biophysical studies using fluorescence spectroscopy.

show abstract

“…). It is known that xantholysin A has antibacterial, antifungal and antitumoral activity (Li et al ., ; Pascual et al ., ).…”

Section: Resultsmentioning

confidence: 97%

Draft whole‐genome sequence of the antibiotic‐producing soil isolate Pseudomonas sp. strain 250J

Molina-Santiago

Udaondo

Ramos

2015

Environ Microbiol Rep

View full text Add to dashboard Cite

Bacteria of the genus Pseudomonas are becoming increasing well known for their ability to produce a wide range of antimicrobial compounds. In a large-scale screening for antibiotic producers, we identified a soil isolate that uses 4-hydroxyphenylacetate as the sole carbon source, Pseudomonas sp. strain 250J, which produces cyclic lipodepsipeptides of the xantholysin family during the stationary phase of growth. The closest relatives of this strain are Pseudomonas mosselii, Pseudomonas soli and Pseudomonas entomophila. Sequencing of the 250J genome allowed us to find the genes relevant to antibiotic production, those which allow utilization of 4-hydroxyphenylacetate as a sole carbon source and a set of genes potentially involved in biocontrol.

show abstract

Pseudomonas soli sp. nov., a novel producer of xantholysin congeners

Cited by 38 publications

References 18 publications

Fluorescent Pseudomonas and cyclic lipopeptide diversity in the rhizosphere of cocoyam (Xanthosoma sagittifolium)

Fluorescent Pseudomonas and cyclic lipopeptide diversity in the rhizosphere of cocoyam (Xanthosoma sagittifolium)

Identification of the Molecular Determinants Involved in Antimicrobial Activity of Pseudodesmin A, a Cyclic Lipopeptide From the Viscosin Group

Draft whole‐genome sequence of the antibiotic‐producing soil isolate Pseudomonas sp. strain 250J

Contact Info

Product

Resources

About