Pseudomonas strains from the Livingston Island, Antarctica: a source of cold-active hydrolytic enzymes

Rubiano-Labrador, Carolina; Acevedo-Barrios, Rosa; Lazaro, Alba García; Bowie, Lilia Ward; Acosta, Ana Karina Támara; Molina, Blanca Mercado

doi:10.18687/laccei2022.1.1.713

Cited by 2 publications

(1 citation statement)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Lipase, protease, amylase, cellulose, urease and beta-glucosidase are some of the reported enzymes from Pseudomonas sp. ( Choo et al, 1998 ; Salwan et al, 2010 ; Jain et al, 2017 ; Salwoom et al, 2019 ; Salwan et al, 2020 ; Rubiano-Labrador et al, 2022 ). The stability of secondary structures in cold temperature were studied and determined by Gianese et al (2001) .…”

Section: Adaptation Strategies – a Facet Of Versatilitymentioning

confidence: 99%

Cold adapted Pseudomonas: ecology to biotechnology

Chauhan,

Kimothi,

Sharma

et al. 2023

Front. Microbiol.

View full text Add to dashboard Cite

The cold adapted microorganisms, psychrophiles/psychrotolerants, go through several modifications at cellular and biochemical levels to alleviate the influence of low temperature stress conditions. The low temperature environments depend on these cold adapted microorganisms for various ecological processes. The ability of the microorganisms to function in cold environments depends on the strategies directly associated with cell metabolism, physicochemical constrains, and stress factors. Pseudomonas is one among such group of microorganisms which is predominant in cold environments with a wide range of ecological and biotechnological applications. Bioformulations of Pseudomonas spp., possessing plant growth promotion and biocontrol abilities for application under low temperature environments, are well documented. Further, recent advances in high throughput sequencing provide essential information regarding the prevalence of Pseudomonas in rhizospheres and their role in plant health. Cold adapted species of Pseudomonas are also getting recognition for their potential in biodegradation and bioremediation of environmental contaminants. Production of enzymes and bioactive compounds (primarily as an adaptation mechanism) gives way to their applications in various industries. Exopolysaccharides and various biotechnologically important enzymes, produced by cold adapted species of Pseudomonas, are making their way in food, textiles, and pharmaceuticals. The present review, therefore, aims to summarize the functional versatility of Pseudomonas with particular reference to its peculiarities along with the ecological and biotechnological applications.

show abstract

Section: Adaptation Strategies – a Facet Of Versatilitymentioning

confidence: 99%

Cold adapted Pseudomonas: ecology to biotechnology

Chauhan,

Kimothi,

Sharma

et al. 2023

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

Antibiofilm activity of Morganella morganii JB8F and Pseudomonas fluorescens JB3B compound to control single and multi-species of aquaculture pathogens

Vanessa,

Waturangi,

Yulandi

et al. 2024

BMC Microbiol

View full text Add to dashboard Cite

Background Indonesia is a country that uses half or more aquatic foods as protein intake. The increased production in aquaculture industries might cause several problems, such as bacterial disease resulting in mass mortality and economic losses. Antibiotics are no longer effective because aquaculture pathogens can form biofilm. Biofilm is a microbial community that aggregates and firmly attaches to living or non-living surfaces. Biofilm formation can be caused by environmental stress, the presence of antibiotics, and limited nutrients. Therefore, it is important to explore antibiofilm to inhibit biofilm formation and/or eradicate mature biofilm. Phyllosphere bacteria can produce bioactive compounds for antimicrobial, antibiofilm, and anti-quorum sensing. Three aquaculture pathogens were used in this study, such as Aeromonas hydrophila , Streptococcus agalactiae , and Vibrio harveyi . Results Pseudomonas fluorescens JB3B and Morganella morganii JB8F extracts could disrupt single and multi-species biofilms. Both extracts could inhibit single biofilm formation from one to seven days of incubation time. We confirmed the destruction activity on multi-species biofilm using light microscope and scanning electron microscope. Using GC-MS analysis, indole was the most active fraction of the P. fluorescens JB3B extracts and octacosane from the M. morganii JB8F extract. We also conducted a toxicity test using brine shrimp lethality assay on P. fluorescens JB3B and M. morganii JB8F extracts. P. fluorescens JB3B, M. morganii JB8F, and a mixture of both extracts were confirmed non-toxic according to the LC 50 value of the brine shrimp lethality test. Conclusions P. fluorescens JB3B and M. morganii JB8F phyllosphere extracts had antibiofilm activity to inhibit single biofilm and disrupt single and multi-species biofilm of aquaculture pathogens. Both extracts could inhibit single species biofilm until seven days of incubation. Bioactive compounds that might contribute to antibiofilm properties were found in both extracts, such as indole and phenol. P. fluorescens JB3B, M. morganii JB8F extracts, and mixture of both extracts were non-toxic against Artemia salina . Supplementary Information The online version contains supplementary material available at 10.1186/s12866-024-03544-6.

show abstract

Pseudomonas strains from the Livingston Island, Antarctica: a source of cold-active hydrolytic enzymes

Cited by 2 publications

References 45 publications

Cold adapted Pseudomonas: ecology to biotechnology

Cold adapted Pseudomonas: ecology to biotechnology

Antibiofilm activity of Morganella morganii JB8F and Pseudomonas fluorescens JB3B compound to control single and multi-species of aquaculture pathogens

Contact Info

Product

Resources

About