Pseudomonas stutzeri and related species undergo natural transformation

Carlson, Curtis A.; Pierson, Leland S.; Rosen, J.; Ingraham, John L.

doi:10.1128/jb.153.1.93-99.1983

Cited by 153 publications

(77 citation statements)

References 29 publications

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“…The results of these experiments provide a standardized procedure in which H. pylori cells can be reproducibly transformed by PCR products of known length. These findings were consistent with observations involving other organisms (39,40) that transformation efficiency for very short fragments was much reduced compared with longer fragments.…”

Section: Frequency Of Transformation Of H Pylori Strain 26695 By Pcrsupporting

confidence: 93%

“…In Bacillus subtilis, shearing of donor DNA from (mean) 28.5 kb to 4.5 kb decreased transformation by 100-fold, and further shearing to 2 kb reduced transformation efficiency another 100-fold (40). In Pseudomonas stutzeri, transformation frequencies did not differ when donor chromosomal DNA fragments were between 10 and 60 kb, but decreased 10-fold for fragments between 1 and 10 kb (39). For Acinetobacter calcoaceticus, transformation frequency was directly related to transforming fragment (between 40 and 10 kb) length (2).…”

Section: Discussionmentioning

confidence: 93%

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Plastic cells and populations: DNA substrate characteristics inHelicobacter pyloritransformation define a flexible but conservative system for genomic variation

Levine¹,

Lin²,

Emara³

et al. 2007

FASEB j.

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Helicobacter pylori, bacteria that colonize the human gastric mucosa, are naturally competent for transformation by exogenous DNA, and show a panmictic population structure. To understand the mechanisms involved in its horizontal gene transfer, we sought to define the interval required from exposure to substrate DNA until DNA uptake and expression of a selectable phenotype, as well as the relationship of transforming fragment length, concentration, homology, symmetry, and strandedness, to the transformation frequency. We provide evidence that natural transformation in H. pylori differs in efficiency among wild-type strains but is saturable and varies with substrate DNA length, symmetry, strandedness, and species origin. We show that H. pylori cells can be transformed within one minute of contact with DNA, by DNA fragments as small as 50 bp, and as few as 5 bp on one flank of a selectable single nucleotide mutation is sufficient substrate for recombination of a transforming fragment, and that double-stranded DNA is the preferred (1000-fold >single-stranded) substrate. The high efficiency of double-stranded DNA as transformation substrate, in conjunction with strain-specific restriction endonucleases suggests a model of short-fragment recombination favoring closest relatives, consistent with the observed H. pylori population biology.

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Section: Frequency Of Transformation Of H Pylori Strain 26695 By Pcrsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 93%

Plastic cells and populations: DNA substrate characteristics inHelicobacter pyloritransformation define a flexible but conservative system for genomic variation

Levine¹,

Lin²,

Emara³

et al. 2007

FASEB j.

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show abstract

“…However, two pieces of evidence caution against concluding panmixis from mathematical linkage equilibrium in this case. Firstly, fluorescent pseudomonads appear not to be naturally competent (Carlson et al 1983) and to date all bacterial populations that approach linkage equilibrium are naturally competent (Lorenz & Wackemagel 1994), with the possible exception of a local population of Burkholderia cepacia (Wise et al 1995). Secondly, from a theoretical point of view, a group that undergoes no largescale recombination should show stronger correlation between the mismatch scores based on two sets of genetic markers, than a group that undergoes frequent mixis.…”

Section: Genefic and Ecotypic Structurementioning

confidence: 99%

Genetic and ecotypic structure of a fluorescent Pseudomonas population

Haubold

Rainey

1996

Molecular Ecology

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Fluorescent pseudomonads are among the most numerous bacteria found on plant surfaces and the activity of certain isolates can affect plant growth. In 1993, 108 fluorescent Pseudomonas isolates were collected on a single sampling occasion from the leaves of sugar beet plants grown at the Oxford University Field Station, Wytham. Isolates were obtained from 54 different leaves, from nine plants, and characterized using 10 allozyme and 23 biotype markers. Statistical analysis of the combined data revealed five biotypic traits which permitted a rational classification of the sample. Analysis of the allozyme data showed that the population was in overall linkage disequilibrium. Clonality was also observed after subdivision of allozyme data along spatial and habitat levels. However, two genetically defined subgroups were in linkage equilibrium which suggests the possibility of frequent large-scale recombination among certain isolates. A significant correlation between isolate distribution and habitat (leaf type and plot) indicates that the population has ecotypic structure

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“…These kinds of gene variation mechanisms may frequently occur in P. stutzeri which is a species with high rearranged chromosomes and no long-range conservation of genetic map (Ginard et al 1997). Moreover, P. stutzeri is capable of natural transformation resulting in genomic diversification by recombination (Carlson et al 1983;Sikorski et al 2002a,b).…”

Section: Discussionmentioning

confidence: 99%

Genomovar assignment of Pseudomonas stutzeri populations inhabiting produced oil reservoirs

et al. 2014

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Oil reservoirs are specific habitats for the survival and growth of microorganisms in general. Pseudomonas stutzeri which is believed to be an exogenous organism inoculated into oil reservoirs during the process of oil production was detected frequently in samples from oil reservoirs. Very little is known, however, about the distribution and genetic structure of P. stutzeri in the special environment of oil reservoirs. In this study, we collected 59 P. stutzeri 16S rRNA gene sequences that were identified in 42 samples from 25 different oil reservoirs and we isolated 11 cultured strains from two representative oil reservoirs aiming to analyze the diversity and genomovar assignment of the species in oil reservoirs. High diversity of P. stutzeri was observed, which was exemplified in the detection of sequences assigned to four known genomovars 1, 2, 3, 20 and eight unknown genomic groups of P. stutzeri. The frequent detection and predominance of strains belonging to genomovar 1 in most of the oil reservoirs under study indicated an association of genomovars of P. stutzeri with the oil field environments.

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Pseudomonas stutzeri and related species undergo natural transformation

Cited by 153 publications

References 29 publications

Plastic cells and populations: DNA substrate characteristics inHelicobacter pyloritransformation define a flexible but conservative system for genomic variation

Plastic cells and populations: DNA substrate characteristics inHelicobacter pyloritransformation define a flexible but conservative system for genomic variation

Genetic and ecotypic structure of a fluorescent Pseudomonas population

Genomovar assignment of Pseudomonas stutzeri populations inhabiting produced oil reservoirs

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