Pseudomonas turukhanskensis sp. nov., isolated from oil-contaminated soils

Abstract

A bacterial strain named IB1.1T was isolated in a screening of hydrocarbon-degrading bacteria from oil-contaminated soils on the territory of the Turukhansk District of Krasnoyarsk Krai, East Siberia, Russia. The 16S rRNA gene sequence had 98.7 % identity with respect to the closest phylogenetic relative, Pseudomonas granadensis F-278,770T, and the next most closely related species with 98.6 % similarity was Pseudomonaspunonensis, suggesting that IB1.1T should be classified within the genus Pseudomonas. The analysis of housekeeping genes rpoB, rpoD and gyrB showed similarities lower than 90 % in all cases with respect to the closest relatives, confirming its phylogenetic affiliation. The strain showed a polar flagellum. The respiratory quinone was Q9. The major fatty acids were 16 : 1ω7c/16 : 1ω6c (summed feature 3), 18 : 1ω7c and 16 : 0. The strain was oxidase- and catalase-positive, but the arginine dihydrolase system was not present. Nitrate reduction, urease and β-galactosidase production, and aesculin hydrolysis were negative. The temperature range for growth was 4-34 °C, and the strain could grow at pH 11. The DNA G+C content was 58.5 mol%. DNA-DNA hybridization results showed values of less than 30 % relatedness with respect to the type strains of the eight most closely related species. Therefore, the dataset of genotypic, phenotypic and chemotaxonomic data support the classification of strain IB1.1T into a novel species of the genus Pseudomonas, for which the name Pseudomonasturukhanskensis sp. nov. is proposed. The type strain is IB1.1T (=VKM B-2935T=CECT 9091T).

Capacity of Pseudomonas Strains to Degrade Hydrocarbons, Produce Auxins and Maintain Plant Growth under Normal Conditions and in the Presence of Petroleum Contaminants

“…Their characteristics are presented in Tables 1 and 2. A description of the Pseudomonas turukhanskensis IB 1.1 strain was reported by Korshunova et al [25], while the P. plecoglossicida 2.4-D strain was described in the article by Chetverikov et al [34]. The presence of petroleum hydrocarbons in the soil is known to make mineral nutrients less accessible for plants [35].…”

“…For example, a model system consisting of bacteria (Sinorhizobium meliloti) capable of associative nitrogen fixation, hydrocarbon oxidation, and the production of auxins in combination with a grass (Sorghum bicolor) has been successfully used for the remediation of soils contaminated with polycyclic aromatic hydrocarbons [24]. Testing free living bacteria of Pseudomonas as hormone producers and plant growth promoters under conditions of contamination with petroleum is of no less interest than the study of associative bacteria, since the capacity for the active decomposition of hydrocarbons and resistance to adverse environmental conditions have been detected in many representatives of this genus [25][26][27].…”

Pseudomonas versuta sp. nov., isolated from Antarctic soil

“…To establish precisely the phylogenetic affiliation of the novel isolates, in addition to the 16S rRNA gene sequence analysis, we also studied the three housekeeping genes more commonly used in the phylogenetic analysis of Pseudomonas species, rpoD, rpoB and gyrB genes [1,22,31,32,33,38,54]. The phylogenetic analysis of the concatenated rpoD, rpoB and gyrB genes sequences was performed with the same methodology as for the 16S rRNA gene and showed that the strains L10.10 T , A4R1.5 and A4R1.12 clustered in a separate branch related to a group formed by the type strains of the closely related species P. weihenstephanensis, P.…”

Diversity of bacteria associated with corn roots inoculated with Canadian woodland soils, and description of Pseudomonas aylmerense sp. nov.

“…Analysis of respiratory quinones of the novel strains showed the presence of Q9 (87%) and Q8 (10%). Q9 as the major ubiquinone of strains S1E40 T and S1E44 is consistent with Pseudomonas species [53, 54]. …”