Pseudooceanicola algae sp. nov., isolated from the marine macroalga Fucus spiralis, shows genomic and physiological adaptations for an algae-associated lifestyle

Wolter, Laura; Wietz, Matthias; Ziesche, Lisa; Breider, Sven; Leinberger, Janina; Daniel, Rolf; Schulz, Stefan

doi:10.1016/j.syapm.2020.126166

Cited by 19 publications

(10 citation statements)

References 91 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Polysaccharides constitute a major fraction of the algae-derived organic matter, with important roles in nutrient cycles and microbial metabolism (Hehemann et al, 2014;Arnosti et al, 2021). Consequently, diverse bacteria are specialized for the degradation of algal polysaccharides, colonization of algal surfaces and other types of biological interactions (van der Loos et al, 2019;Ferrer-González et al, 2020;Wolter et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

CAZymes in Maribacter dokdonensis 62–1 From the Patagonian Shelf: Genomics and Physiology Compared to Related Flavobacteria and a Co-occurring Alteromonas Strain

et al. 2021

Self Cite

View full text Add to dashboard Cite

Carbohydrate-active enzymes (CAZymes) are an important feature of bacteria in productive marine systems such as continental shelves, where phytoplankton and macroalgae produce diverse polysaccharides. We herein describe Maribacter dokdonensis 62–1, a novel strain of this flavobacterial species, isolated from alginate-supplemented seawater collected at the Patagonian continental shelf. M. dokdonensis 62–1 harbors a diverse array of CAZymes in multiple polysaccharide utilization loci (PUL). Two PUL encoding polysaccharide lyases from families 6, 7, 12, and 17 allow substantial growth with alginate as sole carbon source, with simultaneous utilization of mannuronate and guluronate as demonstrated by HPLC. Furthermore, strain 62-1 harbors a mixed-feature PUL encoding both ulvan- and fucoidan-targeting CAZymes. Core-genome phylogeny and pangenome analysis revealed variable occurrence of these PUL in related Maribacter and Zobellia strains, indicating specialization to certain “polysaccharide niches.” Furthermore, lineage- and strain-specific genomic signatures for exopolysaccharide synthesis possibly mediate distinct strategies for surface attachment and host interaction. The wide detection of CAZyme homologs in algae-derived metagenomes suggests global occurrence in algal holobionts, supported by sharing multiple adaptive features with the hydrolytic model flavobacterium Zobellia galactanivorans. Comparison with Alteromonas sp. 76-1 isolated from the same seawater sample revealed that these co-occurring strains target similar polysaccharides but with different genomic repertoires, coincident with differing growth behavior on alginate that might mediate ecological specialization. Altogether, our study contributes to the perception of Maribacter as versatile flavobacterial polysaccharide degrader, with implications for biogeochemical cycles, niche specialization and bacteria-algae interactions in the oceans.

show abstract

Section: Introductionmentioning

confidence: 99%

CAZymes in Maribacter dokdonensis 62–1 From the Patagonian Shelf: Genomics and Physiology Compared to Related Flavobacteria and a Co-occurring Alteromonas Strain

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…The occurrence of pCff1-related plasmids in the pathogenic strains of Curtobacterium and Clavibacter strains appears to be related to their virulence. The important role of plasmids has been shown for many phytopathogenic bacteria [102][103][104][105]. The analysis of, and homology search on, the genes of those plasmids indicated their presence mainly in virulent strains with pathogenicity confirmed.…”

Section: Curtobacterium Plasmids and Pathogenicitymentioning

confidence: 99%

Curtobacterium spp. and Curtobacterium flaccumfaciens: Phylogeny, Genomics-Based Taxonomy, Pathogenicity, and Diagnostics

Evseev

Lukianova

Tarakanov

et al. 2022

CIMB

View full text Add to dashboard Cite

The genus of Curtobacterium, belonging to the Microbacteriaceae family of the Actinomycetales order, includes economically significant pathogenic bacteria of soybeans and other agricultural crops. Thorough phylogenetic and full-genome analysis using the latest genomic data has demonstrated a complex and contradictory taxonomic picture within the group of organisms classified as the Curtobacterium species. Based on these data, it is possible to delineate about 50 new species and to reclassify a substantial part of the Curtobacterium strains. It is suggested that 53 strains, including most of the Curtobacterium flaccumfaciens pathovars, can compose a monophyletic group classified as C. flaccumfaciens. A genomic analysis using the most recent inventory of bacterial chromosomal and plasmid genomes deposited to GenBank confirmed the possible role of Microbacteriaceae plasmids in pathogenicity and demonstrated the existence of a group of related plasmids carrying virulence factors and possessing a gene distantly related to DNA polymerase found in bacteriophages and archaeal and eukaryotic viruses. A PCR diagnostic assay specific to the genus Curtobacterium was developed and tested. The presented results assist in the understanding of the evolutionary relations within the genus and can lay the foundation for further taxonomic updates.

show abstract

“…The genus Pseudooceanicola belonging to the family Rhodobacteraceae of the class Alphaproteobacteria , was first proposed by Lai et al to accommodate aerobic or facultatively anaerobic, Gram-stain-negative, rod-shaped, non-motile, oxidase-positive and catalase-positive bacteria [1–6]. To date, the genus Pseudooceanicola includes 12 species with validly published names: Pseudooceanicola antarcticus (type species), Pseudooceanicola atlanticus , Pseudooceanicola batsensis, Pseudooceanicola marinus , Pseudooceanicola nanhaiensis, Pseudooceanicola nitratireducens , Pseudooceanicola flagellatus , Pseudooceanicola aestuarii , Pseudooceanicola algae , Pseudooceanicola onchidii , Pseudooceanicola pacificus and Pseudooceanicola lipolyticus (https://lpsn.dsmz.de/genus/pseudooceanicola), which were isolated from sea water, epidermis of a marine invertebrate, surface water of Jiulong River, sediments and so on [1–11]. All these species are characterized chemotaxonomically by the presence of ubiquinone 10 (Q-10) as the major respiratory quinone and summed feature 8 (C 18 : 1 ω 7 c and/or C 18 : 1 ω 6 c ), C 16 : 0 , 11-methyl C 18 : 1 ω 7 c and C 12 : 0 3-OH as predominant fatty acids [1].…”

Section: Full-textmentioning

confidence: 99%

“…dsmz. de/ genus/ pseudooceanicola), which were isolated from sea water, epidermis of a marine invertebrate, surface water of Jiulong River, sediments and so on [1][2][3][4][5][6][7][8][9][10][11]. All these species are characterized chemotaxonomically by the presence of ubiquinone 10 (Q-10) as the major respiratory quinone and summed feature 8 (C 18 : 1 ω7c and/or C 18 : 1 ω6c), C 16 : 0 , 11-methyl C 18 : 1 ω7c and C 12 : 0 3-OH as predominant fatty acids [1].…”

mentioning

confidence: 99%

Pseudooceanicola endophyticus sp. nov., a novel endophytic bacterium isolated from bark Aegiceras corniculatum

Zheng

Chen

Yan

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

A Gram-stain-negative, aerobic, short-rod-shaped bacterium, designated strain CBS1P-1T, was isolated from a surface-sterilized bark of Aegiceras corniculatum. Growth of strain CBS1P-1T was observed with between 0 and 12.0 % (w/v) NaCl (optimally with 5.0 %) and at between pH 6.0–9.0. It grew at temperatures between 25–37 °C (optimum, 30 °C). Chemotaxonomic analysis showed that ubiquinone-10 was the respiratory quinone. The lipids comprised diphosphatidylglycerol, phosphatidylglycerol, an unidentified glycolipid, an unidentified phospholipid and an unidentified aminolipid. The major fatty acids of strain CBS1P-1T were C18 : 1 ω7c, C16 : 0 and C19 : 0 cyclo ω8c. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain CBS1P-1T was most related to Pseudooceanicola antarcticus CGMCC 1.12662T with a sequence similarity of 96.5 %. The average nucleotide identity and digital DNA–DNA hybridization values between strain CBS1P-1T and P. antarcticus 1.12662T were 77.5 and 21.1 %, respectively. The G+C content of the genomic DNA was 67.3 mol%. Based on phylogenetic, chemotaxonomic and phenotypic data, strain CBS1P-1T is considered to represent a novel species of the genus Pseudooceanicola , for which the name Pseudooceanicola endophyticus is proposed. The type strain is CBS1P-1T (=KCTC 62836T=CGMCC 1.13743T).

show abstract

Pseudooceanicola algae sp. nov., isolated from the marine macroalga Fucus spiralis, shows genomic and physiological adaptations for an algae-associated lifestyle

Cited by 19 publications

References 91 publications

CAZymes in Maribacter dokdonensis 62–1 From the Patagonian Shelf: Genomics and Physiology Compared to Related Flavobacteria and a Co-occurring Alteromonas Strain

CAZymes in Maribacter dokdonensis 62–1 From the Patagonian Shelf: Genomics and Physiology Compared to Related Flavobacteria and a Co-occurring Alteromonas Strain

Curtobacterium spp. and Curtobacterium flaccumfaciens: Phylogeny, Genomics-Based Taxonomy, Pathogenicity, and Diagnostics

Pseudooceanicola endophyticus sp. nov., a novel endophytic bacterium isolated from bark Aegiceras corniculatum

Contact Info

Product

Resources

About