Pseudopodium extension and amoeboid locomotion in Dictyostelium discoideum: Possible autowave behaviour of F-actin

Vicker, Michael G.; Xiang, Wei; Plath, P; Wosniok, Werner

doi:10.1016/s0167-2789(96)00224-2

Cited by 19 publications

(16 citation statements)

References 87 publications

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“…3A, right side). Such filegrane patterns within F‐actin wavefronts at cell boundaries have previously been noted in fixed cells [9,10].…”

Section: Resultssupporting

confidence: 73%

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F‐actin assembly in Dictyostelium cell locomotion and shape oscillations propagates as a self‐organized reaction–diffusion wave

Vicker

2001

FEBS Letters

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The crawling locomotion and shape of eukaryotic cells have been associated with the stochastic molecular dynamics of actin and its protein regulators, chiefly Arp2/3 and Rho family GTPases, in making a cytoskeleton meshwork within cell extensions. However, the cell's actin-dependent oscillatory shape and extension dynamics may also yield insights into locomotory mechanisms. Confocal observations of live Dictyostelium cells, expressing a green fluorescent protein^actin fusion protein, demonstrate oscillating supramolecular patterns of filamentous actin throughout the cell, which generate pseudopodia at the cell edge. The distinctively dissipative spatio-temporal behavior of these structures provides strong evidence that reversible actin filament assembly propagates as a self-organized, chemical reaction^diffusion wave. ß

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“…3A, right side). Such filegrane patterns within F‐actin wavefronts at cell boundaries have previously been noted in fixed cells [9,10].…”

Section: Resultssupporting

confidence: 73%

“…Cells are vibrating bodies. Dictyostelium 's cell surface movements may be quantitatively described and modelled as transient superpositioned wave modes of differing power and frequency [9,10]. Classical, physical standing and travelling wave patterns characterize the cell surface.…”

Section: Introductionmentioning

confidence: 99%

F‐actin assembly in Dictyostelium cell locomotion and shape oscillations propagates as a self‐organized reaction–diffusion wave

Vicker

2001

FEBS Letters

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“…The presence of wave-like behavior, and hence excitability, in the actin cytoskeleton of Dictyostelium was first reported by Vicker and co-workers [9] who imaged fixed cells stained by phalloidin-rhodamine. Subsequent observations in live cells have confirmed the existence of these cytoskeletal waves [10–13].…”

Section: Excitable Behavior In Cellsmentioning

confidence: 95%

Biased excitable networks: how cells direct motion in response to gradients

Iglesias

Devreotes²

2012

Current Opinion in Cell Biology

127

130

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The actin cytoskeleton in motile cells has many of the hallmarks of an excitable medium, including the presence of propagating waves. This excitable behavior can account for the spontaneous migration of cells. A number of reports have suggested that the chemoattractant-mediated signaling can bias excitability, thus providing a means by which cell motility can be directed. In this review, we discuss some of these observations and theories proposed to explain them. We also suggest a mechanism for cell polarity that can be incorporated into the existing framework.

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“…Similar to traditional traction-mediated cytofission, wave-mediated fission occurs in oversized multinucleate D. discoideum cells that we generated by electric-pulse-induced cell fusion (8,9). The structure and dynamics of actin waves in D. discoideum are well investigated (10)(11)(12)(13). They move across the substrate-attached membrane of the cell (basal waves) and show hallmarks of an excitable system (14)(15)(16).…”

mentioning

confidence: 88%

How cortical waves drive fission of motile cells

Flemming

Font

Alonso

et al. 2020

Proc. Natl. Acad. Sci. U.S.A.

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Cytokinesis—the division of a cell into two daughter cells—is a key step in cell growth and proliferation. It typically occurs in synchrony with the cell cycle to ensure that a complete copy of the genetic information is passed on to the next generation of daughter cells. In animal cells, cytokinesis commonly relies on an actomyosin contractile ring that drives equatorial furrowing and separation into the two daughter cells. However, also contractile ring-independent forms of cell division are known that depend on substrate-mediated traction forces. Here, we report evidence of an as yet unknown type of contractile ring-independent cytokinesis that we termed wave-mediated cytofission. It is driven by self-organized cortical actin waves that travel across the ventral membrane of oversized, multinucleatedDictyostelium discoideumcells. Upon collision with the cell border, waves may initiate the formation of protrusions that elongate and eventually pinch off to form separate daughter cells. They are composed of a stable elongated wave segment that is enclosed by a cell membrane and moves in a highly persistent fashion. We rationalize our observations based on a noisy excitable reaction–diffusion model in combination with a dynamic phase field to account for the cell shape and demonstrate that daughter cells emerging from wave-mediated cytofission exhibit a well-controlled size.

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Pseudopodium extension and amoeboid locomotion in Dictyostelium discoideum: Possible autowave behaviour of F-actin

Cited by 19 publications

References 87 publications

F‐actin assembly in Dictyostelium cell locomotion and shape oscillations propagates as a self‐organized reaction–diffusion wave

F‐actin assembly in Dictyostelium cell locomotion and shape oscillations propagates as a self‐organized reaction–diffusion wave

Biased excitable networks: how cells direct motion in response to gradients

How cortical waves drive fission of motile cells

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