Pseudorhodobacter antarcticus sp. nov., isolated from Antarctic intertidal sandy sediment, and emended description of the genus Pseudorhodobacter Uchino et al. 2002 emend. Jung et al. 2012

Abstract

A Gram-negative, aerobic, non-motile, pink-pigmented and rod-shaped strain, designated ZS3-33T, was isolated from Antarctic intertidal sandy sediment. The strain grew optimally at 15 °C and with 1.0 % (w/v) NaCl. It reduced nitrate to nitrite and hydrolysed Tween 20. It could not produce bacteriochlorophyll a. The predominant cellular fatty acid was C18 : 1ω7c and the predominant respiratory quinone was Q-10. The major polar lipids were phosphatidylglycerol, phosphatidylcholine, two unidentified aminophospholipids and an unidentified aminolipid. Analyses of 16S rRNA gene sequences revealed that strain ZS3-33T belonged to the genus Pseudorhodobacter , showing 97.4 % similarity to the type strain of Pseudorhodobacter ferrugineus and 95.3 % similarity to the type strain of Pseudorhodobacter aquimaris . Levels of gyrB gene sequence similarity between strain ZS3-33T and the type strains of P. ferrugineus and P. aquimaris were 87.6 and 81.7 %, respectively. DNA–DNA relatedness between strain ZS3-33T and P. ferrugineus DSM 5888T was 56.6 %. The genomic DNA G+C content of strain ZS3-33T was 57.1 mol%. Based on data from this polyphasic study, strain ZS3-33T represents a novel species of the genus Pseudorhodobacter , for which the name Pseudorhodobacter antarcticus sp. nov. is proposed. The type strain is ZS3-33T ( = CGMCC 1.10836T = KCTC 23700T). An emended description of the genus Pseudorhodobacter Uchino et al. 2002 emend. Jung et al. 2012 is also proposed.

Pseudorhodobacter psychrotolerans sp. nov., a psychrotolerant bacterium isolated from terrestrial soil, and emended description of the genus Pseudorhodobacter

“…The description is as given by Uchino et al (2002), Jung et al (2012), Chen et al (2013) and Lee et al (2013) with the following amendment. Some species grow facultatively aerobically.…”

“…1). However, physiological characteristics such as hydrolysis of macromolecules, enzyme activities and acid production, genomic data compared based on calculation of ANI values and genome-to-genome distances, and the different composition of polar lipids distinguished strain PAMC 27389 T from other type strains of the genus pH range for growth (optimum) 5.5-9.0 (6.5-7.0) 5.5-9.5 (7.0-7.5) 5.0-10.0 (7.5)* 6.0-9.5 (7.5) 6.0-10.0 (8.0-9.0) API ZYM test Alkaline phosphatase *NaCl tolerance and PH range for growth data were taken from Chen et al (2013).…”

“…Members of the genus Pseudorhodobacter have been isolated from intertidal sandy sediments, seawater and wood falls (Uchino et al, 2002;Jung et al, 2012;Chen et al, 2013;Lee et al, 2013). The genomic G+C contents of the type strains of species of the genus Pseudorhodobacter range from 57.1 to 61.6 mol%.…”

“…Pure cultures of the bacterial isolates were deposited with the Polar and Alpine Microbial Collection (PAMC; Lee et al, 2012) and preserved as glycerol suspensions (20 %, v/v, in distilled water) at 280 8C. In this study, one of these strains, PAMC 27389 T , was routinely cultured on modified TYS agar medium [0.5 % tryptone (Oxoid), 0.1 % yeast extract and 0.5 % sea salt], with a reduced concentration of sea salt compared with standard TYS medium [0.5 % tryptone (Oxoid), 0.1 % yeast extract and 3 % sea salt (Chen et al, 2013)] at 15 8C after determination of the optimal temperature for growth. For comparative analyses with strain PAMC 27389 T , four type strains of Pseudorhodobacter species, Pseudorhodobacter wandonensis KCTC 23672 T , Pseudorhabdobacter antarcticus KCTC 23700 T , Pseudorhabdobacter aquimaris KCTC 23043 T and P. ferrugineus LMG 22047 T , were purchased from Korean Collection of type Cultures (KCTC) and Laboratory of Microbiology Gent Bacteria Collection (LMG) and used as reference strains following cultivation under comparable conditions as for PAMC 27389 T .…”

Pseudorhodobacter collinsensis sp. nov., isolated from a till sample of an icecap front

“…T belonged to the genus Pseudorhodobacter, with the closest relatives being Pseudorhodobacter wandonensis WT-MW11 T (96.9 % 16S rRNA gene sequence similarity), Pseudorhodobacter antarcticus The genus Pseudorhodobacter, which belongs to the class Alphaproteobacteria, was first proposed by Uchino et al 1992;Jung et al, 2012;Chen et al, 2013;Lee et al, 2013). The purpose of the present study was to determine the taxonomic position of strain 4-T-34 T by using a polyphasic approach and to propose that the strain represents a novel species of the genus Pseudorhodobacter.…”