Pseudosterase activity-based specific detection of human serum albumin on gel

Kumar, Deepak; Bhattacharyya, Rajasri; Banerjee, Dibyajyoti

doi:10.1016/j.talanta.2020.121906

Cited by 9 publications

(5 citation statements)

References 59 publications

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Section: Hsa Enzymatic Propertiesmentioning

confidence: 99%

“…Interestingly, a specific method to detect the HSA (pseudo-)esterase activity has been developed [ 123 ]. This method is based on a staining protocol based on the use of: (i) Fast Blue BB (0.12%) dye to stain the (pseudo-) esterase activity of HSA both on agarose and Native PAGE; (ii) 2-naphthyl acetate, an ester substrate of HSA, and (iii) neostigmine, a selective acetyl-cholinesterase inhibitor [ 123 ]. This method could find application in either HSA pharmaceutical preparation (e.g., to check adulteration of human milk with cow milk) [ 123 , 124 , 125 ] or in diagnostic detection (e.g., to highlight the (pseudo-)esterase activity of HSA in urine representing a sign of microalbuminuria) [ 123 , 126 ].…”

Section: Hsa Enzymatic Propertiesmentioning

confidence: 99%

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Serum Albumin: A Multifaced Enzyme

Masi

Ascenzi

2021

IJMS

127

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Human serum albumin (HSA) is the most abundant protein in plasma, contributing actively to oncotic pressure maintenance and fluid distribution between body compartments. HSA acts as the main carrier of fatty acids, recognizes metal ions, affects pharmacokinetics of many drugs, provides the metabolic modification of some ligands, renders potential toxins harmless, accounts for most of the anti-oxidant capacity of human plasma, and displays esterase, enolase, glucuronidase, and peroxidase (pseudo)-enzymatic activities. HSA-based catalysis is physiologically relevant, affecting the metabolism of endogenous and exogenous compounds including proteins, lipids, cholesterol, reactive oxygen species (ROS), and drugs. Catalytic properties of HSA are modulated by allosteric effectors, competitive inhibitors, chemical modifications, pathological conditions, and aging. HSA displays anti-oxidant properties and is critical for plasma detoxification from toxic agents and for pro-drugs activation. The enzymatic properties of HSA can be also exploited by chemical industries as a scaffold to produce libraries of catalysts with improved proficiency and stereoselectivity for water decontamination from poisonous agents and environmental contaminants, in the so called “green chemistry” field. Here, an overview of the intrinsic and metal dependent (pseudo-)enzymatic properties of HSA is reported to highlight the roles played by this multifaced protein.

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Section: Hsa Enzymatic Propertiesmentioning

confidence: 99%

Section: Hsa Enzymatic Propertiesmentioning

confidence: 99%

Serum Albumin: A Multifaced Enzyme

Masi

Ascenzi

2021

IJMS

127

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“…GHSA was reported to provide more relevant and immediate glucose information relative to HbA1c in children, adolescents, and pregnant women. , Therefore, many attempts have been made to search for GHSA detection strategies. − Previously, liquid chromatography, , affinity chromatography, , colorimetry, Raman spectroscopy, and immunochemistry were studied to detect a GHSA level; however, such methods are costly and time-consuming. More convenient and faster techniques such as a lateral flow assay, an electrochemical immunoassay, specific gel electrophoresis, and optical biosensors ,− are introduced.…”

Section: Introductionmentioning

confidence: 99%

“… 19 − 21 Previously, liquid chromatography, 22 , 23 affinity chromatography, 24 , 25 colorimetry, 26 Raman spectroscopy, 27 and immunochemistry 28 were studied to detect a GHSA level; however, such methods are costly and time-consuming. More convenient and faster techniques such as a lateral flow assay, 17 an electrochemical immunoassay, 29 specific gel electrophoresis, 30 and optical biosensors 20 , 31 − 35 are introduced.…”

Section: Introductionmentioning

confidence: 99%

Binding of Apo and Glycated Human Serum Albumins to an Albumin-Selective Aptamer-Bound Graphene Quantum Dot Complex

et al. 2023

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Diabetes mellitus is a chronic metabolic disease involving continued elevated blood glucose levels. It is a leading cause of mortality and reduced life expectancy. Glycated human serum albumin (GHSA) has been reported to be a potential diabetes biomarker. A nanomaterial-based aptasensor is one of the effective techniques to detect GHSA. Graphene quantum dots (GQDs) have been widely used in aptasensors as an aptamer fluorescence quencher due to their high biocompatibility and sensitivity. GHSA-selective fluorescent aptamers are first quenched upon binding to GQDs. The presence of albumin targets results in the release of aptamers to albumin and consequently fluorescence recovery. To date, the molecular details on how GQDs interact with GHSA-selective aptamers and albumin remain limited, especially the interactions of an aptamer-bound GQD (GQDA) with an albumin. Thus, in this work, molecular dynamics simulations were used to reveal the binding mechanism of human serum albumin (HSA) and GHSA to GQDA. The results show the rapid and spontaneous assembly of albumin and GQDA. Multiple sites of albumins can accommodate both aptamers and GQDs. This suggests that the saturation of aptamers on GQDs is required for accurate albumin detection. Guanine and thymine are keys for albumin-aptamer clustering. GHSA gets denatured more than HSA. The presence of bound GQDA on GHSA widens the entrance of drug site I, resulting in the release of open-chain glucose. The insight obtained here will serve as a base for accurate GQD-based aptasensor design and development.

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“…Biosensor methods have gained significant popularity as analytical devices in various fields due to their fast response, low cost, and high sensitivity and specificity. Several biological sensing techniques have been explored as alternative tools for detecting albumin in urine and blood samples [ 18 , 19 , 20 , 21 ]. The need for a highly selective, rapid, simple, and cost-effective biosensing platform has spurred interest in nanomaterials with unique optical, electronic, and catalytic properties that make them ideal candidates for advanced biosensing systems [ 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

Development of a High-Accuracy, Low-Cost, and Portable Fluorometer with Smartphone Application for the Detection of Urinary Albumin towards the Early Screening of Chronic Kidney and Renal Diseases

Pinrod,

Chawjiraphan,

Segkhoonthod

et al. 2023

Biosensors

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This study presents the development of a portable fluorometer with a smartphone application designed to facilitate the early screening of chronic kidney and renal diseases by enabling the sensitive detection of urinary albumin. Utilizing a fluorescence-based aptasensor, the device achieved a linear calibration curve (0.001–1.5 mg/mL) with a linearity of up to 0.98022 and a detection limit of 0.203 µg/mL for human serum albumin (HSA). The analysis of 130 urine samples demonstrated comparable performance between this study’s fluorometer, a commercial fluorometer, and the standard automated method. These findings validate the feasibility of the portable fluorometer and aptasensor combination as a reliable instrument for the sensitive and specific measurement of HSA in urine samples. Moreover, the fluorometer’s portability offers potential applications in portable point-of-care testing, enhancing its utility in clinical settings for early disease screening.

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Pseudosterase activity-based specific detection of human serum albumin on gel

Cited by 9 publications

References 59 publications

Serum Albumin: A Multifaced Enzyme

Serum Albumin: A Multifaced Enzyme

Binding of Apo and Glycated Human Serum Albumins to an Albumin-Selective Aptamer-Bound Graphene Quantum Dot Complex

Development of a High-Accuracy, Low-Cost, and Portable Fluorometer with Smartphone Application for the Detection of Urinary Albumin towards the Early Screening of Chronic Kidney and Renal Diseases

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