The purpose of this study was to investigate the current status and influencing factors of night shift nurses’ sense of occupational benefit, and to explore the influence of psychological resilience on the sense of occupational benefit. 2022 from August to October, a cross-sectional survey was conducted on 719 night shift nurses using a general information questionnaire, psychological resilience scale, and sense of occupational benefit scale. A total of 719 valid questionnaires were collected. The score of night shift nurses’ sense of occupational benefit was (137.40 ± 21.10) and psychological resilience score was (65.63 ± 17.75). Age (β = 3.359, P < .05) was significantly correlated with sense of occupational benefit. Education (β = 3.586, P < .05) was significantly correlated with the sense of occupational benefit, and whether or not they had participated in outbreak prevention and control (β = −2.321, P < .05) was significantly correlated with the sense of occupational benefit. Similarly, psychological resilience (β = 0.859, P < .05) was significantly associated with the sense of occupational benefit. Night shift nurses’ sense of occupational benefit was moderate to high, and interventions should be taken to enhance the sense of occupational benefit based on nurses’ age, education, whether they have participated in epidemic prevention and control, and psychological resilience.