Psoriasis-like Inflammation Induced in an Air-pouch Mouse Model

Abstract

Background/Aim: The pathway of initiation of psoriasis comprises the differentiation and infiltration of Thelper 17 (Th17) cells into the skin, characterized by the production of interleukin 17A and 17F (IL-17A/IL-17F) among other cytokines, resulting in a downstream cascade of events. Due to the lack of simplicity in psoriasis models, we aimed to develop an easily and rapidly inducible mouse model for the IL-23/IL-17 pathway with quick readouts from a straightforward lavaging process and with detectable cytokine levels. Materials and Methods: We utilized the 6-day airpouch mouse model, injected with a combination of anti-CD3, IL-23 and L-1β. At 24, 48 and 72 h, intra-pouch secretion of IL-17A, IL-17F and C-X-C motif chemokine ligand 1 were measured Skin biopsies were collected and immune cell infiltration evaluated, and intra-pouch immune cells were isolated and analyzed. Results: The combination of anti-CD3, IL-23 with and without IL-1 significantly increased intrapouch levels of IL-17A/IL-17F at 24 and 72 h, triggering a downstream production of C-X-C motif chemokine ligand 1. The cytokines were detectable even 72 h post-induction. T-cell receptor beta was down-regulated on CD4 + and CD8 + T-cells, indicating intra-pouch T-cell activation. Αnti-CD3 induced CD3+ cell migration into the subcutis and the lining tissue surrounding the cavity of the air pouch, where in the latter, a similar distribution pattern of Il17a mRNA-expressing cells was also observed. However, no Th17 cell differentiation nor changes in IL-17A + granulocytes were observed. Conclusion: The induced air-pouch mouse model induced with a cocktail of anti-CD3, IL-23 with or without IL-1β is able to mirror the IL-23/IL-17 axis of psoriasis-like inflammation characterized by immune cell infiltration and cytokine secretion.

Interleukin-17 Links Inflammatory Cross-Talks Between Comorbid Psoriasis and Atherosclerosis

“…Studies using psoriasis-like animal models induced by air-pouch ( 80 ) or imiquimod ( 81 ) confirmed that psoriasis was elicited by the IL-23/IL-17 axis. Naive T cells are differentiated into Th1 cells by IL-12 and interferon (IFN)-γ or Th17 cells by IL-6, TGF-β and IL-23 respectively ( 82 , 83 ).…”

Common Wound Irrigation Solutions Produce Different Responses in Infected vs Sterile Host Tissue: Murine Air Pouch Infection Model

“…Five-micron hematoxylin and eosin sections were evaluated for signs of healing and host cell status. All slides were reviewed by a trained, blinded pathologist and scored for comparisons based on both his expertise and previous validated applications of inflammation and cytotoxicity in air pouch models [ 24 , 27 , 28 ].…”