Psycho-emotional stress - A cause of coronary artery disease

Abstract

Psycho-emotional stress is one of the risk factors for metabolic syndrome and related diseases. Hence, this study has designed to the effect of psycho-emotional stress in the form of immobilization stress in rabbits. In this study, we used 12 male New Zealand White rabbits (2.5-3 kg body weight), Six animals for hanging fixation and another six as control. Blood samples were taken before hanging (0 minute) and at 60, 120 minutes. Serum was separated and used for the estimation of various biochemical parameters. Hanging fixation appears to produce significant increase in malondialdehyde and lipid profile levels. The non-enzymatic antioxidants like reduced glutathione, vitamin E and ceruloplasmin were found to be decrease and the level of enzymatic antioxidant like glutathione peroxidase was increased significantly. The results reveal that the hanging fixation may develop coronary artery disease. This is the area, which should be evaluated further by carrying out chronic studies in hung stress and by histopathological studies.

Glutamic Acid Decarboxylase Concentration Changes in Response to Stress and Altered Availability of Glutamic Acid in Rabbit (Oryctolagus cuniculus) Brain Limbic Structures

“…This may suggest that in some regions of the brain, increased GABA levels appear together with reduced Glu level, which would confirm the results of studies by Wu and colleagues [ 59 ] on hypothalamic tissue. In turn, other studies, such as Chellappan and colleagues [ 25 ], indicate that blocking the synthesis of nitric oxide in the prefrontal cortex resulted in a significant increase in GAD concentration, which the authors described as explaining the depletion of GABA resources in the cerebral cortex. All the previously mentioned studies are especially important for rats and rabbits in the context of maintaining the welfare of these animals, due to their frequent use in laboratories.…”

“…of 2 mL of saline solution (0.9% NaCl); Group 2 (stress)—The stress reaction was triggered by a 30 min suspension of the rabbit at a height of 40 cm above the ground in a properly prepared stand ( Figure 1 ). This model was previously described in detail [ 25 ]; Group 3 (Glu)—Injection ( i.p. ) of Glu (G1626, Sigma-Aldrich, St. Louis, USA), dose 5.07 mg/kg b.w.…”

“…Group 2 (stress)—The stress reaction was triggered by a 30 min suspension of the rabbit at a height of 40 cm above the ground in a properly prepared stand ( Figure 1 ). This model was previously described in detail [ 25 ];…”

“…Moreover, a significant correlation between the concentration of these hormones and brain neurotransmitters has recently been found in growing male rabbits [ 20 ]. Several previous investigations have revealed that the GABA used as a neurotransmitter plays a brain developmental function in animals [ 15 , 23 , 24 , 25 ]. With respect to this research topic and in relation to the welfare of smaller mammals, the results are insufficient, leaving a wide field for further research at the molecular level.…”