Abstract:The aim of this study was to examine the post-injury psychological impact looking to avoid sources of health issues. We have analyzed differences in the stress, anxiety, and depression levels between male and female football players who have suffered at least one injury during the last season played, with respect to similar players who have not been injured. A descriptive cross-sectional design was used. The participants were 187 football players and data on the player's injuries were collected. A self-reported questionnaire collected number, type, and severity of injuries sustained during the previous sports season (twelve months), and also the player's current situation, being injured or not injured. To evaluate the psychological variables, the DASS-21 questionnaire was used. Results indicated that the male injured players presented anxiety levels higher than those who were non-injured. When we observe the female players' data, the stress levels of the non-injured ones are higher than those of the injured players. Regarding the non-injured athletes, results showed that the female non-injured players presented values in anxiety higher than those corresponding to the non-injured male players. Thus, sport injury affects mental health issues such as anxiety and stress, and in a different way regarding gender.