BACKGROUND: Infertility and its treatment can have a significant impact on the individual's quality of life (QoL). Fertility-specific QoL measures can be obtained through the FertiQoL (Boivin, Takefman, & Braverman, 2011), a questionnaire with six-subscales that consider different core aspects of the person's wellbeing and way of behaving during treatment. OBJECTIVE: Examine the psychometric properties of all six-subscales of the Italian FertiQoL in a national sample of infertile women and explore the effects of the ART treatment phases on different features of fertility-related QoL. METHOD: 323 women, in three different treatment stages, completed the FertiQoL (Diagnostic, Stimulation, Transfer). Raw data were subject to Confirmatory Factor Analysis (CFA), and a structural equation modelling was used to validate the hypothesized model. RESULTS: CFA shows a good fit of the data to the FertiQoL hierarchical model (chi-square/df = 1.989, CFI=0.88, RMSEA=0.055). After the deletion of 2 items, all FertiQoL scales have good or more than acceptable internal consistency. SEM analysis showed that the ART treatment phase was positively associated with fertility-related QoL scores both in the Relational (β= 0.14, p<0.05) and in the Tolerability (β= =0.17, p<0.05) subscales. CONCLUSION: All scales of the Italian FertiQoL version maintain good psychometric characteristics; Tolerability and Relational subscales are sensitive to the stage of treatment and may provide relevant information for the medical staff.