Background: Hospitalized patients Spiritual Needs Questionnaire (HPSNQ) has been developed in Iran. This paper uses the classical test theory (CTT) to test the validity of the HPSNQ. Using Item response theory, a scale can be constructed with items that can be interpreted regarding the spiritual needs of patients revealing evidence that calls for more effective health interventions regarding meeting the spiritual needs of patients. Analysis of the hospitalized patients Spiritual Needs Questionnaire using Item Response Theory with the aim of providing insight into the functioning of the questionnaire to improve the psychometric robustness of the instrument. Methods: This analytical study was carried out in 2018 on 301 subjects hospitalized in non-acute wards of hospitals in Shiraz, Iran. The analysis was carried out using Item Response Theory: The dimensionality, assessing the items parameters evaluation, and the scale construction were assessed using Cronbach’s alpha, item-to-total correlations and confirmatory factor analysis was done for investigation of dimensionality. The Gradual Response Model of Samejima was used to estimate the item parameters, and the MPLUS software was utilized to analyses the data.Results: Findings revealed that HPSNQ scales are highly unidimensional. In this study, regarding the dimension of “relationship with God”, very high discriminations were observed among all items, whereas the items in “peaceful environment” had relatively low discriminations, hence was not as informative as the items of “relationship to god”.Conclusions: Whereas the psychometric properties of the HPSNQ was generally supported from an IRT perspective, there was also evidence of further improvement for this measure. It seems that the nature of the construct under study, has a significant impact on the items’ discrimination and information value; however, the wording correction or deletion of certain items or even the response options used in this tool may significantly increase the reliability of the tool.