Online self-learning became widespread after the onset of the COVID-19 pandemic, and during this period, learners’ self-leadership became an important measure of their competency. This study aims to verify the validity and reliability of the tool by translating the Abbreviated Self-Leadership Questionnaire (ASLQ) into Korean. Through web-based surveys, 286 nursing students from two university nursing colleges participated in the study. Cronbach’s α, correlation, and confirmatory factor analyses were used to verify the nine-item ASLQ. The factor loading values ranged from 0.378 to 0.892. The ASLQ model fit had a comparative fit index of 0.944, root mean square error of approximation of 0.093, and standardized root mean residual of 0.069. The ASLQ showed a statistically significant positive correlation with the original self-leadership, emotional intelligence, and self-efficacy questionnaires. Additionally, structural, convergent, and criterion validity were also evident. The reliability of the ASLQ was 0.846 for Cronbach’s α (the three sub-dimensions were 0.836, 0.732, and 0.734). This study verified the validity and reliability of the ASLQ and confirmed that it could be used to measure the self-leadership of nursing students at a time when self-leadership is crucial.