Amidst rapid societal changes and increasing urbanization, human connectivity with nature has declined, exacerbating public health concerns. This study assesses the efficacy of Shinrin-yoku, or ‘forest bathing’, in Wuyishan National Park as a simple and effective method to counteract the adverse health effects of contemporary lifestyles. Employing repeated-measures analysis of variance, forty-one participants were observed over three days across eight distinct forest settings. Techniques included eye-tracking for visual attention and soundscape perception assessments via questionnaires. Physiological responses were gauged through heart rate variability and skin conductance, while psychological evaluations utilized the Profile of Mood States (POMS) and Positive and Negative Affect Schedule (PANAS). Findings revealed that (1) natural soundscapes—especially birdsong, flowing water, wind, and bamboo raft sounds—and visual elements, such as distant mountains, streams, trees, Danxia landforms, tea gardens, and bamboo views, play pivotal roles in regulating heart rate variability, reducing arousal, and enhancing stress adaptation. Additionally, cultural landscapes, such as classical music and ancient structures, bolster parasympathetic activity. (2) Natural and cultural auditory stimuli, including flowing water and classical music, coupled with visual features, such as Danxia landforms, streams, distant mountains, lawns, and guide signs, effectively induce positive mood states, regulate mood disturbances, and enhance psychological well-being across diverse forest settings. These findings underscore the significant health benefits of immersive natural experiences and advocate for integrating forest-based wellness programs into public health strategies, offering compelling evidence for enriching life quality through nature engagement.