The purpose of this study is to examine the physiological responses to predict the psychological level of perceived fear. Thirty male and female college students (15 male and 15 female, mean age: 22.6±1.24) participated in the experiment. EDA (electrodermal activity), ECG (electrocardiogram), and facial EMG (electromyogram) as physiological signals were measured on the subjects' hands and face for 60sec before presentation of emotional stimulus and for 120sec during presentation of stimulus. Experimental conditions consisted of emotional condition where fear was induced by a threatening film clip and neutral condition where no emotion was provoked by a neutral film clip. After presentation of the stimulus, subjects rated their experienced emotion on the emotion assessment scale. Analysis of psychological responses was performed to examine appropriateness (label of the subjects' experienced emotion) and effectiveness (intensity of their experienced emotion). In the analysis of physiological responses, the selected features were skin conductance level (SCL), skin conductance response (SCR), number of skin conductance response (NSCR), R-R interval (R-R), heart rate (HR), respiration (RESP), activation in the bilateral corrugators (COR), and bilateral orbicularis oris' (ORB). The results showed that the psychological responses to stimulus were appropriate and effective. Physiological responses showed significant increases in all features except R-R and ORB during fear condition compared to baseline condition. Also, the perceived level of fear was positively correlated with SCL, SCR, and ORB. Our result offer that the users' perceived emotion i.e., individual differences of psychological responses must be considered to recognize human emotions by physiological signals in HCI.