Psychosocial risk factors are conditions present in the work environment that are related to the organization of work, its social environment, work content and the performance of the task that can affect the health of workers. This research aims to determine the psychosocial factors at work and stress in the medical staff of a public institution. Research under the positivist, quantitative, observational, descriptive and cross-sectional paradigm. The population and census sample consisted of 15 medical profession workers. In the collection of information, three questionnaires from the Battery of instruments for the evaluation of psychosocial risk factors of the Ministry of Social Protection, Colombia (2010) were applied. The results show that women predominated (80.0%), where (46.7%) was single, with an average age and seniority of 32 years and 10 years respectively. As for psychosocial work factors, high and very high risk levels were found in 53.3%, with extra-work conditions being the most unfavorable, associated with the economic situation of the family group (80.0%), characteristics of the home and the environment (73.3%) and home-work-home displacement (73.3.%). On the other hand, 40.0% of the personnel studied perceived high and very high risk levels of stress. Concluding that the population is exposed to occupational psychosocial factors, with the appearance of revealing symptoms of stress. Therefore, the use of corrective measures that help minimize possible damage to health is recommended.