Psychrobacillus lasiicapitis sp. nov., isolated from the head of an ant (Lasius fuliginosus)

Abstract

A novel Gram-stain-positive, motile, endospore-forming, rod-shaped bacterium, designated strain NEAU-3TGS17, was isolated from the head of an ant (Lasius fuliginosus). The isolate grew at 0-35 °C (optimum 28-30 °C), at pH 6.0-11.0 (optimum pH 7.0-8.0) and with 0-6 % (w/v) NaCl (optimum 0 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NEAU-3TGS17 belonged to the genus Psychrobacillus. Sequence similarities between strain NEAU-3TGS17 and members of the genus Psychrobacillus with validly published names (Psychrobacillus psychrotolerans DSM 11706, Psychrobacillus insolitus DSM 5, Psychrobacillus psychrodurans DSM 11713 and Psychrobacillus soli NBRC 110600) were 98.4-99.1 %. DNA-DNA relatedness values between strain NEAU-3TGS17 and its closest relatives were below 70 %. The major cellular fatty acids (>5 %) were iso-C14 : 0, anteiso-C15 : 0, C16 : 0 and iso-C15 : 0. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The cell-wall peptidoglycan type was A4β with ornithine as the diamino acid and the predominant menaquinones were MK-8 and some MK-7. The DNA G+C content was 35.8 mol%. On the basis of phenotypic data and phylogenetic inference, strain NEAU-3TGS17 was classified as representing a novel species in the genus Psychrobacillus, for which the name Psychrobacillus lasiicapitis sp. nov. is proposed. The type strain is NEAU-3TGS17 (=DSM 100484=CGMCC 1.15308).

Psychrobacillus glaciei sp. nov., a psychrotolerant species isolated from an Antarctic iceberg

“…The DNA G+C content of strain PB01 T calculated from the complete genome sequence was 36.0 mol%, which falls within the range reported for other members of the genus Psychrobacillus (35-37 mol%) ( Table 1) [1,4,5].…”

“…The final polar lipids were recovered into the chloroform phase and analysed by two-dimensional TLC using Kieselgel 60 F254 plates (Merck). Polar lipids were separated in the first direction with a mixture of chloroform/ *Data from their previous works [1,4,5] respectively, except for PB01 T . †DPG, diphosphatidylglycerol ; PG, phosphatidylglycerol; PE, phosphatidylethanolamine.…”

“…net): Psychrobacillus insolitus, Psychrobacillus psychrotolerans, Psychrobacillus psychrodurans, Psychrobacillus soli and Psychrobacillus lasiicapitis [1,4,5]. Species of the genus Psychrobacillus are commonly found in a broad range of soil environments and even in the head of ant [4,5]. Some Psychrobacillus species have beneficial capabilities such as oil degradation [4].…”

The microbiota of the Lasius fuliginosus – Pella laticollis myrmecophilous interaction

“…We cannot rule out the possibility that the Mycobacterium found in bacterial communities associated with ants is involved in these defensive mechanisms. Bacteria-derived antifungal compounds have recently been described from the head and body of L. fuliginosus (Liu et al 2016a(Liu et al , 2016bYe et al 2017;Shen et al 2017;Brinker et al 2019). The higher abundances of the genera listed above are in accordance with the profiling of ant microbiota performed to date.…”

Bacterial Microbiota Isolated from Cysts of Globodera rostochiensis (Nematoda: Heteroderidae)

“…As efforts have been moved towards expanding the source of microorganisms involving the more complex systems in nature [ 57 ], nematodes and their related bacterial microbiota present the next biological system to explore the taxonomic diversity of soil bacteria. Nematodes, especially cyst nematodes, are a significant natural source of microorganisms due to their long persistence in soil and the specific environmental conditions inside and outside of the closed area of cysts, in which diverse bacteria are hidden.…”