Psychrophilic and mesophilic anaerobic treatment of synthetic dairy wastewater with long chain fatty acids: Process performances and microbial community dynamics

Liu, Yuchen; Ramiro-García, Javier; Paulo, Lara M.; Braguglia, Camilla Maria; Gagliano, Maria Cristina; O’Flaherty, Vincent

doi:10.1016/j.biortech.2023.129124

Cited by 17 publications

(3 citation statements)

References 49 publications

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“…Another factor limiting the efficiency of low-temperature gas production is the excessive accumulation of toxic substances ( Lu et al, 2023 ). This is because the low-temperature tolerance of methanogenic microorganisms is weak compared with that of organic acid-producing microorganisms, which leads to the fact that organic acids cannot be decomposed into methane promptly ( Holohan et al, 2022 ; Liu et al, 2023 ), and this also causes a negative feedback effect in the digestive system ( Kotsyurbenko, 2005 ). So how improving the low-temperature tolerance of methanogenic microorganisms is one of the effective ways to solve the problem of low-temperature anaerobic digestion.…”

Section: Introductionmentioning

confidence: 99%

Effect of bioaugmentation on gas production and microbial community during anaerobic digestion in a low-temperature fixed-bed reactor

Wang,

An,

Wang

et al. 2024

Front. Microbiol.

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Low temperature is one of the limiting factors for anaerobic digestion in cold regions. To improve the efficiency of anaerobic digestion for methane production in stationary reactors under low-temperature conditions, and to improve the structure of the microbial community for anaerobic digestion at low temperatures. We investigated the effects of different concentrations of exogenous Methanomicrobium (10, 20, 30%) and different volumes of carbon fiber carriers (0, 10, 20%) on gas production and microbial communities to improve the performance of low-temperature anaerobic digestion systems. The results show that the addition of 30% exogenous microorganisms and a 10% volume of carbon fiber carrier led to the highest daily (128.15 mL/g VS) and cumulative (576.62 mL/g VS) methane production. This treatment effectively reduced the concentrations of COD and organic acid, in addition to stabilizing the pH of the system. High-throughput sequencing analysis revealed that the dominant bacteria under these conditions were Acidobacteria and Firmicutes and the dominant archaea were Candidatus_Udaeobacter and Methanobacterium. While the abundance of microorganisms that metabolize organic acids was reduced, the functional abundance of hydrogenophilic methanogenic microorganisms was increased. Therefore, the synergistic effect of Methanomicrobium bioaugmentation with carbon fiber carriers can significantly improve the performance and efficiency of low-temperature anaerobic fermentation systems.

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Section: Introductionmentioning

confidence: 99%

Effect of bioaugmentation on gas production and microbial community during anaerobic digestion in a low-temperature fixed-bed reactor

Wang,

An,

Wang

et al. 2024

Front. Microbiol.

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“…However, these socioeconomically and ecologically preferable substrates, such as lignocellulose (Karrabi et al, 2023 ) or long chain fatty acids (Liu et al, 2023 ), are often difficult to degrade for microorganisms. Chemical, physical and biological pre‐treatment technologies can enhance biogas production from these feedstocks and are thus inevitable (Shrestha et al, 2017 ; Wagner et al, 2018 ; Zheng et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

Pre‐treatment with Trichoderma viride: Towards a better understanding of its consequences for anaerobic digestion

Markt,

Prem,

Lackner

et al. 2024

Environ Microbiol Rep

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Understanding and optimising biological pre‐treatment strategies for enhanced bio‐methane production is a central aspect in second‐generation biofuel research. In this regard, the application of fungi for pre‐treatment seems highly promising; however, understanding the mode of action is crucial. Here, we show how aerobic pre‐treatment of crystalline cellulose with the cellulolytic Trichoderma viride affects substrate degradability during mesophilic, anaerobic digestion. It could be demonstrated that fungal pre‐treatment resulted in a slightly reduced substrate mass. Nevertheless, no significant impact on the overall methane yield was found during batch fermentation. Short chain organic acids accumulation, thus, overall degradation dynamics including methane production kinetics were affected by the pre‐treatment as shown by Gompertz modelling. Finally, 16S rRNA amplicon sequencing followed by ANCOM‐BC resulted in up to 53 operative taxonomic units including fermentative, syntrophic and methanogenic taxa, whereby their relative abundances were significantly affected by fungal pre‐treatment depending on the duration of the pre‐treatment. The results demonstrated the impact of soft rot fungal pre‐treatment of cellulose on subsequent anaerobic cellulose hydrolysis as well as on methanogenic activity. To the best of our knowledge, this is the first study to investigate the direct causal effects of pre‐treatment with T. viride on basic but crucial anaerobic digestion parameters in a highly standardised approach.

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“…Many studies have also compared the performance of low-and moderate-temperature AD [22]. The feasibility of long-term continuous LT-AD treatment of long-chain fatty acid (LCFA)-containing wastewater using acclimated biomass has been reported, with Methanocorpusculum playing a crucial role in LCFA degradation during both psychrophilic and mesophilic treatments [23]. It was observed by Hui Y et al [22] that the initial inhibition of methane production was observed in LT-AD under antibiotic stress (e.g., oxytetracycline); however, it was later stimulated at 35 • C; and in comparison to MT-AD, the dominant acetoclastic methanogens Methanosaeta gradually declined and were replaced by methylotrophic Methanomethylovorans in LT-AD.…”

Section: Introductionmentioning

confidence: 99%

Characteristics of Biogas Production Activity and Microbial Community during Sub-Moderate Temperature Anaerobic Digestion of Wastewater

Wu,

Zhang,

Zhao

et al. 2023

Fermentation

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Sub-moderate temperature (ranging from low to moderate temperature) anaerobic digestion (AD) could balance fermentation efficiency and energy input. We investigated biogas production and the microbial community in wastewater AD at sub-moderate (15 °C, 20 °C, and 25 °C) and moderate (35 °C; control group) temperatures with the organic loading rate (OLR) incrementally increased over 200 days. The impact of temperature on biogas production was found to be minimal at a low OLR but became more significant at a high OLR. Notably, a temperature threshold ranging from 15 °C to 20 °C exerted a strong inhibitory effect on biogas production and disrupted the microbial community. And, SMT-AD is deemed by this study to be the optimal application strategy of wastewater with low temperature and low OLR. Bacterial richness was positively and linearly related to temperature. There is a relevance between methane production and archaeal diversity under the influence of temperature and OLR. Temperature and OLR shaped the ecological function of predominant bacteria. Anaerolineales, Thermotogales, and Lactobacillales were strongly influenced by temperature. Synergistales had a synergistic relationship with Desulfovibrionales. Clostridiales was responsible for acetate and butyrate production and closely related to Lactobacillales. Acetoclastic Methanosaetaceae was the predominant methanogen. Methanogens could survive and maintain their population even though methanogenesis was limited under high OLRs and low temperatures.

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Psychrophilic and mesophilic anaerobic treatment of synthetic dairy wastewater with long chain fatty acids: Process performances and microbial community dynamics

Cited by 17 publications

References 49 publications

Effect of bioaugmentation on gas production and microbial community during anaerobic digestion in a low-temperature fixed-bed reactor

Effect of bioaugmentation on gas production and microbial community during anaerobic digestion in a low-temperature fixed-bed reactor

Pre‐treatment with Trichoderma viride: Towards a better understanding of its consequences for anaerobic digestion

Characteristics of Biogas Production Activity and Microbial Community during Sub-Moderate Temperature Anaerobic Digestion of Wastewater

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